This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McCarthy, J. E. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McCarthy, J. E. G.

 Previous Article  |  Next Article 

Microbiology and Molecular Biology Reviews, December 1998, p. 1492-1553, Vol. 62, No. 4
1092-2172/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Posttranscriptional Control of Gene Expression in Yeast

John E. G. McCarthy*

Posttranscriptional Control Group, Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology (UMIST), Manchester M60 1QD, United Kingdom

Studies of the budding yeast Saccharomyces cerevisiae have greatly advanced our understanding of the posttranscriptional steps of eukaryotic gene expression. Given the wide range of experimental tools applicable to S. cerevisiae and the recent determination of its complete genomic sequence, many of the key challenges of the posttranscriptional control field can be tackled particularly effectively by using this organism. This article reviews the current knowledge of the cellular components and mechanisms related to translation and mRNA decay, with the emphasis on the molecular basis for rate control and gene regulation. Recent progress in characterizing translation factors and their protein-protein and RNA-protein interactions has been rapid. Against the background of a growing body of structural information, the review discusses the thermodynamic and kinetic principles that govern the translation process. As in prokaryotic systems, translational initiation is a key point of control. Modulation of the activities of translational initiation factors imposes global regulation in the cell, while structural features of particular 5' untranslated regions, such as upstream open reading frames and effector binding sites, allow for gene-specific regulation. Recent data have revealed many new details of the molecular mechanisms involved while providing insight into the functional overlaps and molecular networking that are apparently a key feature of evolving cellular systems. An overall picture of the mechanisms governing mRNA decay has only very recently begun to develop. The latest work has revealed new information about the mRNA decay pathways, the components of the mRNA degradation machinery, and the way in which these might relate to the translation apparatus. Overall, major challenges still to be addressed include the task of relating principles of posttranscriptional control to cellular compartmentalization and polysome structure and the role of molecular channelling in these highly complex expression systems.

* Mailing address: Posttranscriptional Control Group, Department of Biomolecular Sciences, UMIST, Manchester M60 1QD, United Kingdom. Phone: (44)161-200-8916. Fax: (44)161-200-8918. E-mail: J.McCarthy{at}umist.ac.uk.

Microbiology and Molecular Biology Reviews, December 1998, p. 1492-1553, Vol. 62, No. 4
1092-2172/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Whang, M. I., Guerra, N., Raulet, D. H. (2009). Costimulation of Dendritic Epidermal {gamma}{delta} T Cells by a New NKG2D Ligand Expressed Specifically in the Skin. J. Immunol. 182: 4557-4564 [Abstract] [Full Text]  
  • Dimelow, R. J, Wilkinson, S. J (2009). Control of translation initiation: a model-based analysis from limited experimental data. J R Soc Interface 6: 51-61 [Abstract] [Full Text]  
  • Puyaubert, J., Denis, L., Alban, C. (2008). Dual Targeting of Arabidopsis HOLOCARBOXYLASE SYNTHETASE1: A Small Upstream Open Reading Frame Regulates Translation Initiation and Protein Targeting. Plant Physiol. 146: 478-491 [Abstract] [Full Text]  
  • Daran-Lapujade, P., Rossell, S., van Gulik, W. M., Luttik, M. A. H., de Groot, M. J. L., Slijper, M., Heck, A. J. R., Daran, J.-M., de Winde, J. H., Westerhoff, H. V., Pronk, J. T., Bakker, B. M. (2007). The fluxes through glycolytic enzymes in Saccharomyces cerevisiae are predominantly regulated at posttranscriptional levels. Proc. Natl. Acad. Sci. USA 104: 15753-15758 [Abstract] [Full Text]  
  • Sangthong, P., Hughes, J., McCarthy, J. E. G. (2007). Distributed control for recruitment, scanning and subunit joining steps of translation initiation. Nucleic Acids Res 35: 3573-3580 [Abstract] [Full Text]  
  • Gilbert, R. J. C., Gordiyenko, Y., von der Haar, T., Sonnen, A. F.-P., Hofmann, G., Nardelli, M., Stuart, D. I., McCarthy, J. E. G. (2007). Reconfiguration of yeast 40S ribosomal subunit domains by the translation initiation multifactor complex. Proc. Natl. Acad. Sci. USA 104: 5788-5793 [Abstract] [Full Text]  
  • Babendure, J. R., Babendure, J. L., Ding, J.-H., Tsien, R. Y. (2006). Control of mammalian translation by mRNA structure near caps. RNA 12: 851-861 [Abstract] [Full Text]  
  • Shababi, M., Bourque, J., Palanichelvam, K., Cole, A., Xu, D., Wan, X.-F., Schoelz, J. (2006). The ribosomal shunt translation strategy of cauliflower mosaic virus has evolved from ancient long terminal repeats.. J. Virol. 80: 3811-3822 [Abstract] [Full Text]  
  • Rand, J. D., Grant, C. M. (2006). The Thioredoxin System Protects Ribosomes against Stress-induced Aggregation. Mol. Biol. Cell 17: 387-401 [Abstract] [Full Text]  
  • Vasudevan, S., Garneau, N., Tu Khounh, D., Peltz, S. W. (2005). p38 Mitogen-Activated Protein Kinase/Hog1p Regulates Translation of the AU-Rich-Element-Bearing MFA2 Transcript. Mol. Cell. Biol. 25: 9753-9763 [Abstract] [Full Text]  
  • Kuai, L., Das, B., Sherman, F. (2005). A nuclear degradation pathway controls the abundance of normal mRNAs in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 102: 13962-13967 [Abstract] [Full Text]  
  • Churbanov, A., Rogozin, I. B., Babenko, V. N., Ali, H., Koonin, E. V. (2005). Evolutionary conservation suggests a regulatory function of AUG triplets in 5'-UTRs of eukaryotic genes. Nucleic Acids Res 33: 5512-5520 [Abstract] [Full Text]  
  • Steigele, S., Nieselt, K. (2005). Open reading frames provide a rich pool of potential natural antisense transcripts in fungal genomes. Nucleic Acids Res 33: 5034-5044 [Abstract] [Full Text]  
  • Srikantha, T., Zhao, R., Daniels, K., Radke, J., Soll, D. R. (2005). Phenotypic Switching in Candida glabrata Accompanied by Changes in Expression of Genes with Deduced Functions in Copper Detoxification and Stress. Eukaryot Cell 4: 1434-1445 [Abstract] [Full Text]  
  • Arava, Y., Boas, F. E., Brown, P. O., Herschlag, D. (2005). Dissecting eukaryotic translation and its control by ribosome density mapping. Nucleic Acids Res 33: 2421-2432 [Abstract] [Full Text]  
  • Nasim, M. T., Trembath, R. C. (2005). A dual-light reporter system to determine the efficiency of protein-protein interactions in mammalian cells. Nucleic Acids Res 33: e66-e66 [Abstract] [Full Text]  
  • Bjorkbacka, H., Fitzgerald, K. A., Huet, F., Li, X., Gregory, J. A., Lee, M. A., Ordija, C. M., Dowley, N. E., Golenbock, D. T., Freeman, M. W. (2005). The induction of macrophage gene expression by LPS predominantly utilizes Myd88-independent signaling cascades. Physiol. Genomics 19: 319-330 [Abstract] [Full Text]  
  • Malys, N., Carroll, K., Miyan, J., Tollervey, D., McCarthy, J. E. G. (2004). The 'scavenger' m7GpppX pyrophosphatase activity of Dcs1 modulates nutrient-induced responses in yeast. Nucleic Acids Res 32: 3590-3600 [Abstract] [Full Text]  
  • BENARD, L. (2004). Inhibition of 5' to 3' mRNA degradation under stress conditions in Saccharomyces cerevisiae: from GCN4 to MET16. RNA 10: 458-468 [Abstract] [Full Text]  
  • Shor, B., Calaycay, J., Rushbrook, J., McLeod, M. (2003). Cpc2/RACK1 Is a Ribosome-associated Protein That Promotes Efficient Translation in Schizosaccharomyces pombe. J. Biol. Chem. 278: 49119-49128 [Abstract] [Full Text]  
  • Lieto, L. D., Borrego, F., You, C.-h., Coligan, J. E. (2003). Human CD94 Gene Expression: Dual Promoters Differing in Responsiveness to IL-2 or IL-15. J. Immunol. 171: 5277-5286 [Abstract] [Full Text]  
  • Gardocki, M. E., Lopes, J. M. (2003). Expression of the Yeast PIS1 Gene Requires Multiple Regulatory Elements Including a Rox1p Binding Site. J. Biol. Chem. 278: 38646-38652 [Abstract] [Full Text]  
  • Arava, Y., Wang, Y., Storey, J. D., Liu, C. L., Brown, P. O., Herschlag, D. (2003). Genome-wide analysis of mRNA translation profiles in Saccharomycescerevisiae. Proc. Natl. Acad. Sci. USA 100: 3889-3894 [Abstract] [Full Text]  
  • Washburn, M. P., Koller, A., Oshiro, G., Ulaszek, R. R., Plouffe, D., Deciu, C., Winzeler, E., Yates, J. R. III (2003). Protein pathway and complex clustering of correlated mRNA and protein expression analyses in Saccharomycescerevisiae. Proc. Natl. Acad. Sci. USA 100: 3107-3112 [Abstract] [Full Text]  
  • Lee, J., Park, E. H., Couture, G., Harvey, I., Garneau, P., Pelletier, J. (2002). An upstream open reading frame impedes translation of the huntingtin gene. Nucleic Acids Res 30: 5110-5119 [Abstract] [Full Text]  
  • Dunyak, D. S., Everdeen, D. S., Albanese, J. G., Quinn, C. L. (2002). Deletion of Individual mRNA Capping Genes Is Unexpectedly Not Lethal to Candida albicans and Results in Modified mRNA Cap Structures. Eukaryot Cell 1: 1010-1020 [Abstract] [Full Text]  
  • Milone, J., Wilusz, J., Bellofatto, V. (2002). Identification of mRNA decapping activities and an ARE-regulated 3' to 5' exonuclease activity in trypanosome extracts. Nucleic Acids Res 30: 4040-4050 [Abstract] [Full Text]  
  • Uesono, Y., Toh-e, A. (2002). Transient Inhibition of Translation Initiation by Osmotic Stress. J. Biol. Chem. 277: 13848-13855 [Abstract] [Full Text]  
  • Griffin, T. J., Gygi, S. P., Ideker, T., Rist, B., Eng, J., Hood, L., Aebersold, R. (2002). Complementary Profiling of Gene Expression at the Transcriptome and Proteome Levels in Saccharomyces cerevisiae. Mol. Cell. Proteomics 1: 323-333 [Abstract] [Full Text]  
  • Kim, M. J., Kim, J. B., Kim, D. S., Park, S. D. (2002). Glucose-inducible expression of rrg1+ in Schizosaccharomyces pombe: post-transcriptional regulation of mRNA stability mediated by the downstream region of the poly(A) site. Nucleic Acids Res 30: 1145-1153 [Abstract] [Full Text]  
  • Shmulevitz, M., Yameen, Z., Dawe, S., Shou, J., O'Hara, D., Holmes, I., Duncan, R. (2002). Sequential Partially Overlapping Gene Arrangement in the Tricistronic S1 Genome Segments of Avian Reovirus and Nelson Bay Reovirus: Implications for Translation Initiation. J. Virol. 76: 609-618 [Abstract] [Full Text]  
  • Vo, L. T. A., Minet, M., Schmitter, J.-M., Lacroute, F., Wyers, F. (2001). Mpe1, a Zinc Knuckle Protein, Is an Essential Component of Yeast Cleavage and Polyadenylation Factor Required for the Cleavage and Polyadenylation of mRNA. Mol. Cell. Biol. 21: 8346-8356 [Abstract] [Full Text]  
  • Kozak, M. (2001). Constraints on reinitiation of translation in mammals. Nucleic Acids Res 29: 5226-5232 [Abstract] [Full Text]  
  • Ptushkina, M., Berthelot, K., von der Haar, T., Geffers, L., Warwicker, J., McCarthy, J. E. G. (2001). A second eIF4E protein in Schizosaccharomyces pombe has distinct eIF4G-binding properties. Nucleic Acids Res 29: 4561-4569 [Abstract] [Full Text]  
  • Thompson, S. R., Gulyas, K. D., Sarnow, P. (2001). Internal initiation in Saccharomyces cerevisiae mediated by an initiator tRNA/eIF2-independent internal ribosome entry site element. Proc. Natl. Acad. Sci. USA 10.1073/pnas.241286698v1 [Abstract] [Full Text]  
  • Paz, I., Choder, M. (2001). Eukaryotic Translation Initiation Factor 4E-Dependent Translation Is Not Essential for Survival of Starved Yeast Cells. J. Bacteriol. 183: 4477-4483 [Abstract] [Full Text]  
  • Zhou, W., Edelman, G. M., Mauro, V. P. (2001). Transcript leader regions of two Saccharomyces cerevisiae mRNAs contain internal ribosome entry sites that function in living cells. Proc. Natl. Acad. Sci. USA 98: 1531-1536 [Abstract] [Full Text]  
  • Noueiry, A. O., Chen, J., Ahlquist, P. (2000). A mutant allele of essential, general translation initiation factor DED1 selectively inhibits translation of a viral mRNA. Proc. Natl. Acad. Sci. USA 10.1073/pnas.240460897v1 [Abstract] [Full Text]  
  • Hemmings-Mieszczak, M., Hohn, T., Preiss, T. (2000). Termination and Peptide Release at the Upstream Open Reading Frame Are Required for Downstream Translation on Synthetic Shunt-Competent mRNA Leaders. Mol. Cell. Biol. 20: 6212-6223 [Abstract] [Full Text]  
  • ter Kuile, B. H., Sallés, F. J. (2000). The Length of the Combined 3' Untranslated Region and Poly(A) Tail Does Not Control Rates of Glyceraldehyde-3-Phosphate Dehydrogenase mRNA Translation in Three Species of Parasitic Protists. J. Bacteriol. 182: 3587-3589 [Abstract] [Full Text]  
  • Gale, M. Jr., Tan, S.-L., Katze, M. G. (2000). Translational Control of Viral Gene Expression in Eukaryotes. Microbiol. Mol. Biol. Rev. 64: 239-280 [Abstract] [Full Text]  
  • Wyers, F., Minet, M., Dufour, M. E., Vo, L. T. A., Lacroute, F. (2000). Deletion of the PAT1 Gene Affects Translation Initiation and Suppresses a PAB1 Gene Deletion in Yeast. Mol. Cell. Biol. 20: 3538-3549 [Abstract] [Full Text]  
  • Brown, J. T., Yang, X., Johnson, A. W. (2000). Inhibition of mRNA Turnover in Yeast by an xrn1 Mutation Enhances the Requirement for eIF4E Binding to eIF4G and for Proper Capping of Transcripts by Ceg1p. Genetics 155: 31-42 [Abstract] [Full Text]  
  • Wang, Z., Gaba, A., Sachs, M. S. (1999). A Highly Conserved Mechanism of Regulated Ribosome Stalling Mediated by Fungal Arginine Attenuator Peptides That Appears Independent of the Charging Status of Arginyl-tRNAs. J. Biol. Chem. 274: 37565-37574 [Abstract] [Full Text]  
  • ter Kuile, B. H. (1999). Regulation and Adaptation of Glucose Metabolism of the Parasitic Protist Leishmania donovani at the Enzyme and mRNA Levels. J. Bacteriol. 181: 4863-4872 [Abstract] [Full Text]  
  • Paz, I., Abramovitz, L., Choder, M. (1999). Starved Saccharomyces cerevisiae Cells Have the Capacity to Support Internal Initiation of Translation. J. Biol. Chem. 274: 21741-21745 [Abstract] [Full Text]  
  • Richter, J. D. (1999). Cytoplasmic Polyadenylation in Development and Beyond. Microbiol. Mol. Biol. Rev. 63: 446-456 [Abstract] [Full Text]  
  • Hughes, J. M. X., Ptushkina, M., Karim, Md. M., Koloteva, N., von der Haar, T., McCarthy, J. E. G. (1999). Translational Repression by Human 4E-BP1 in Yeast Specifically Requires Human eIF4E as Target. J. Biol. Chem. 274: 3261-3264 [Abstract] [Full Text]  
  • von der Haar, T., Ball, P. D., McCarthy, J. E. G. (2000). Stabilization of Eukaryotic Initiation Factor 4E Binding to the mRNA 5'-Cap by Domains of eIF4G. J. Biol. Chem. 275: 30551-30555 [Abstract] [Full Text]  
  • Karim, M. M., Hughes, J. M. X., Warwicker, J., Scheper, G. C., Proud, C. G., McCarthy, J. E. G. (2001). A Quantitative Molecular Model for Modulation of Mammalian Translation by the eIF4E-binding Protein 1. J. Biol. Chem. 276: 20750-20757 [Abstract] [Full Text]  
  • Xiao, X., Tang, Y.-S., Mackins, J. Y., Sun, X.-L., Jayaram, H. N., Hansen, D. K., Antony, A. C. (2001). Isolation and Characterization of a Folate Receptor mRNA-binding trans-Factor from Human Placenta. EVIDENCE FAVORING IDENTITY WITH HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN E1. J. Biol. Chem. 276: 41510-41517 [Abstract] [Full Text]  
  • Staib, P., Kretschmar, M., Nichterlein, T., Hof, H., Morschhauser, J. (2000). Differential activation of a Candida albicans virulence gene family during infection. Proc. Natl. Acad. Sci. USA 97: 6102-6107 [Abstract] [Full Text]  
  • Noueiry, A. O., Chen, J., Ahlquist, P. (2000). A mutant allele of essential, general translation initiation factor DED1 selectively inhibits translation of a viral mRNA. Proc. Natl. Acad. Sci. USA 97: 12985-12990 [Abstract] [Full Text]  
  • Thompson, S. R., Gulyas, K. D., Sarnow, P. (2001). Internal initiation in Saccharomyces cerevisiae mediated by an initiator tRNA/eIF2-independent internal ribosome entry site element. Proc. Natl. Acad. Sci. USA 98: 12972-12977 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»