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Microbiology and Molecular Biology Reviews, March 2000, p. 34-50, Vol. 64, No. 1
1092-2172/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Metabolic Engineering of Saccharomyces cerevisiae

Simon Ostergaard, Lisbeth Olsson, and Jens Nielsen*

Center for Process Biotechnology, Department of Biotechnology, Technical University of Denmark, DK-2800 Lyngby, Denmark

Comprehensive knowledge regarding Saccharomyces cerevisiae has accumulated over time, and today S. cerevisiae serves as a widley used biotechnological production organism as well as a eukaryotic model system. The high transformation efficiency, in addition to the availability of the complete yeast genome sequence, has facilitated genetic manipulation of this microorganism, and new approaches are constantly being taken to metabolicially engineer this organism in order to suit specific needs. In this paper, strategies and concepts for metabolic engineering are discussed and several examples based upon selected studies involving S. cerevisiae are reviewed. The many different studies of metabolic engineering using this organism illustrate all the categories of this multidisciplinary field: extension of substrate range, improvements of producitivity and yield, elimination of byproduct formation, improvement of process performance, improvements of cellular properties, and extension of product range including heterologous protein production.

* Corresponding author. Mailing address: Center for Process Biotechnology, Department of Biotechnology, Technical University of Denmark, Building 223, DK-2800 Lyngby, Denmark. Phone: 45 45 25 26 96. Fax: 45 45 88 41 48. E-mail: jn{at}ibt.dtu.dk.

Microbiology and Molecular Biology Reviews, March 2000, p. 34-50, Vol. 64, No. 1
1092-2172/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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