This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tjalsma, H. Articles by van Dijl, J. M. Search for Related Content PubMed PubMed Citation Articles by Tjalsma, H. Articles by van Dijl, J. M.

Proteomics of Protein Secretion by Bacillus subtilis: Separating the "Secrets" of the Secretome

Department of Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, 9751 NN Haren,¹ Department of Clinical Chemistry/564, University Medical Centre Nijmegen, 6500 HB Nijmegen,² Department of Pharmaceutical Biology, University of Groningen, 9713 AV Groningen,⁴ Department of Molecular Bacteriology, University of Groningen, 9700 RB Groningen, The Netherlands,⁵ Institut für Mikrobiologie und Molekularbiologie, Ernst-Moritz-Arndt-Universiät Greifswald, D-17487 Greifswald, Germany³

Secretory proteins perform a variety of important "remote-control" functions for bacterial survival in the environment. The availability of complete genome sequences has allowed us to make predictions about the composition of bacterial machinery for protein secretion as well as the extracellular complement of bacterial proteomes. Recently, the power of proteomics was successfully employed to evaluate genome-based models of these so-called secretomes. Progress in this field is well illustrated by the proteomic analysis of protein secretion by the gram-positive bacterium Bacillus subtilis, for which 90 extracellular proteins were identified. Analysis of these proteins disclosed various "secrets of the secretome," such as the residence of cytoplasmic and predicted cell envelope proteins in the extracellular proteome. This showed that genome-based predictions reflect only 50% of the actual composition of the extracellular proteome of B. subtilis. Importantly, proteomics allowed the first verification of the impact of individual secretion machinery components on the total flow of proteins from the cytoplasm to the extracellular environment. In conclusion, proteomics has yielded a variety of novel leads for the analysis of protein traffic in B. subtilis and other gram-positive bacteria. Ultimately, such leads will serve to increase our understanding of virulence factor biogenesis in gram-positive pathogens, which is likely to be of high medical relevance.

This article has been cited by other articles:

• Inacio, J. M., de Sa-Nogueira, I. (2008). Characterization of abn2 (yxiA), Encoding a Bacillus subtilis GH43 Arabinanase, Abn2, and Its Role in Arabino-Polysaccharide Degradation. J. Bacteriol. 190: 4272-4280 [Abstract] [Full Text]  
• Arrell, D. K., Niederlander, N. J., Faustino, R. S., Behfar, A., Terzic, A. (2008). Cardioinductive Network Guiding Stem Cell Differentiation Revealed by Proteomic Cartography of Tumor Necrosis Factor {alpha}-Primed Endodermal Secretome. Stem Cells 26: 387-400 [Abstract] [Full Text]  
• Hyyrylainen, H.-L., Pietiainen, M., Lunden, T., Ekman, A., Gardemeister, M., Murtomaki-Repo, S., Antelmann, H., Hecker, M., Valmu, L., Sarvas, M., Kontinen, V. P. (2007). The density of negative charge in the cell wall influences two-component signal transduction in Bacillus subtilis. Microbiology 153: 2126-2136 [Abstract] [Full Text]  
• Bierne, H., Cossart, P. (2007). Listeria monocytogenes Surface Proteins: from Genome Predictions to Function. Microbiol. Mol. Biol. Rev. 71: 377-397 [Abstract] [Full Text]  
• Chitlaru, T., Gat, O., Grosfeld, H., Inbar, I., Gozlan, Y., Shafferman, A. (2007). Identification of In Vivo-Expressed Immunogenic Proteins by Serological Proteome Analysis of the Bacillus anthracis Secretome. Infect. Immun. 75: 2841-2852 [Abstract] [Full Text]  
• Nijland, R., Heerlien, R., Hamoen, L. W., Kuipers, O. P. (2007). Changing a Single Amino Acid in Clostridium perfringens {beta}-Toxin Affects the Efficiency of Heterologous Secretion by Bacillus subtilis. Appl. Environ. Microbiol. 73: 1586-1593 [Abstract] [Full Text]  
• Wen, F., VanEtten, H. D., Tsaprailis, G., Hawes, M. C. (2007). Extracellular Proteins in Pea Root Tip and Border Cell Exudates. Plant Physiol. 143: 773-783 [Abstract] [Full Text]  
• St. John, F. J., Rice, J. D., Preston, J. F. (2006). Characterization of XynC from Bacillus subtilis subsp. subtilis Strain 168 and Analysis of Its Role in Depolymerization of Glucuronoxylan. J. Bacteriol. 188: 8617-8626 [Abstract] [Full Text]  
• Darmon, E., Dorenbos, R., Meens, J., Freudl, R., Antelmann, H., Hecker, M., Kuipers, O. P., Bron, S., Quax, W. J., Dubois, J.-Y. F., van Dijl, J. M. (2006). A Disulfide Bond-Containing Alkaline Phosphatase Triggers a BdbC-Dependent Secretion Stress Response in Bacillus subtilis.. Appl. Environ. Microbiol. 72: 6876-6885 [Abstract] [Full Text]  
• Bigot, A., Botton, E., Dubail, I., Charbit, A. (2006). A Homolog of Bacillus subtilis Trigger Factor in Listeria monocytogenes Is Involved in Stress Tolerance and Bacterial Virulence.. Appl. Environ. Microbiol. 72: 6623-6631 [Abstract] [Full Text]  
• Sibbald, M. J. J. B., Ziebandt, A. K., Engelmann, S., Hecker, M., de Jong, A., Harmsen, H. J. M., Raangs, G. C., Stokroos, I., Arends, J. P., Dubois, J. Y. F., van Dijl, J. M. (2006). Mapping the Pathways to Staphylococcal Pathogenesis by Comparative Secretomics. Microbiol. Mol. Biol. Rev. 70: 755-788 [Abstract] [Full Text]  
• Heng, N. C. K., Ragland, N. L., Swe, P. M., Baird, H. J., Inglis, M. A., Tagg, J. R., Jack, R. W. (2006). Dysgalacticin: a novel, plasmid-encoded antimicrobial protein (bacteriocin) produced by Streptococcus dysgalactiae subsp. equisimilis. Microbiology 152: 1991-2001 [Abstract] [Full Text]  
• Chitlaru, T., Gat, O., Gozlan, Y., Ariel, N., Shafferman, A. (2006). Differential Proteomic Analysis of the Bacillus anthracis Secretome: Distinct Plasmid and Chromosome CO2-Dependent Cross Talk Mechanisms Modulate Extracellular Proteolytic Activities.. J. Bacteriol. 188: 3551-3571 [Abstract] [Full Text]  
• Nouaille, S., Morello, E., Cortez-Peres, N., Le Loir, Y., Commissaire, J., Gratadoux, J. J., Poumerol, E., Gruss, A., Langella, P. (2006). Complementation of the Lactococcus lactis Secretion Machinery with Bacillus subtilis SecDF Improves Secretion of Staphylococcal Nuclease.. Appl. Environ. Microbiol. 72: 2272-2279 [Abstract] [Full Text]  
• Patterson, H. M., Brannigan, J. A., Cutting, S. M., Wilson, K. S., Wilkinson, A. J., AB, E., Diercks, T., de Jong, R. N., Truffault, V., Folkers, G. E., Kaptein, R. (2005). The Structure of Bypass of Forespore C, an Intercompartmental Signaling Factor during Sporulation in Bacillus. J. Biol. Chem. 280: 36214-36220 [Abstract] [Full Text]  
• Bigot, A., Pagniez, H., Botton, E., Frehel, C., Dubail, I., Jacquet, C., Charbit, A., Raynaud, C. (2005). Role of FliF and FliI of Listeria monocytogenes in Flagellar Assembly and Pathogenicity. Infect. Immun. 73: 5530-5539 [Abstract] [Full Text]  
• Zhang, X.-Z., Cui, Z.-L., Hong, Q., Li, S.-P. (2005). High-Level Expression and Secretion of Methyl Parathion Hydrolase in Bacillus subtilis WB800. Appl. Environ. Microbiol. 71: 4101-4103 [Abstract] [Full Text]  
• Dhalluin, A., Bourgeois, I., Pestel-Caron, M., Camiade, E., Raux, G., Courtin, P., Chapot-Chartier, M.-P., Pons, J.-L. (2005). Acd, a peptidoglycan hydrolase of Clostridium difficile with N-acetylglucosaminidase activity. Microbiology 151: 2343-2351 [Abstract] [Full Text]  
• Albano, M., Smits, W. K., Ho, L. T. Y., Kraigher, B., Mandic-Mulec, I., Kuipers, O. P., Dubnau, D. (2005). The Rok Protein of Bacillus subtilis Represses Genes for Cell Surface and Extracellular Functions. J. Bacteriol. 187: 2010-2019 [Abstract] [Full Text]  
• Converse, S. E., Cox, J. S. (2005). A Protein Secretion Pathway Critical for Mycobacterium tuberculosis Virulence Is Conserved and Functional in Mycobacterium smegmatis. J. Bacteriol. 187: 1238-1245 [Abstract] [Full Text]