This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vemuri, G. N.
Right arrow Articles by Aristidou, A. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vemuri, G. N.
Right arrow Articles by Aristidou, A. A.

Next Article 

Microbiology and Molecular Biology Reviews, June 2005, p. 197-216, Vol. 69, No. 2
1092-2172/05/$08.00+0     doi:10.1128/MMBR.69.2.197-216.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Metabolic Engineering in the -omics Era: Elucidating and Modulating Regulatory Networks

Goutham N. Vemuri1 and Aristos A. Aristidou2*

Center for Molecular BioEngineering, Driftmier Engineering Center, University of Georgia, Athens, Georgia 30605,1 NatureWorks, 15305 Minnetonka Blvd., Minnetonka, Minnesota 553452

The importance of regulatory control in metabolic processes is widely acknowledged, and several enquiries (both local and global) are being made in understanding regulation at various levels of the metabolic hierarchy. The wealth of biological information has enabled identifying the individual components (genes, proteins, and metabolites) of a biological system, and we are now in a position to understand the interactions between these components. Since phenotype is the net result of these interactions, it is immensely important to elucidate them not only for an integrated understanding of physiology, but also for practical applications of using biological systems as cell factories. We present some of the recent "-omics" approaches that have expanded our understanding of regulation at the gene, protein, and metabolite level, followed by analysis of the impact of this progress on the advancement of metabolic engineering. Although this review is by no means exhaustive, we attempt to convey our ideology that combining global information from various levels of metabolic hierarchy is absolutely essential in understanding and subsequently predicting the relationship between changes in gene expression and the resulting phenotype. The ultimate aim of this review is to provide metabolic engineers with an overview of recent advances in complementary aspects of regulation at the gene, protein, and metabolite level and those involved in fundamental research with potential hurdles in the path to implementing their discoveries in practical applications.

* Corresponding author. Mailing address: NatureWorks LLC, 15305 Minnetonka Blvd., Minnetonka, MN 55345. Phone: (952) 742-0495. Fax: (952) 742-0481. E-mail: aristos_aristidou{at}natureworksllc.com.

Microbiology and Molecular Biology Reviews, June 2005, p. 197-216, Vol. 69, No. 2
1092-2172/05/$08.00+0     doi:10.1128/MMBR.69.2.197-216.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Rossouw, D., Olivares-Hernandes, R., Nielsen, J., Bauer, F. F. (2009). Comparative Transcriptomic Approach To Investigate Differences in Wine Yeast Physiology and Metabolism during Fermentation. Appl. Environ. Microbiol. 75: 6600-6612 [Abstract] [Full Text]  
  • Dori-Bachash, M., Dassa, B., Pietrokovski, S., Jurkevitch, E. (2008). Proteome-Based Comparative Analyses of Growth Stages Reveal New Cell Cycle-Dependent Functions in the Predatory Bacterium Bdellovibrio bacteriovorus. Appl. Environ. Microbiol. 74: 7152-7162 [Abstract] [Full Text]  
  • Ishii, N., Nakahigashi, K., Baba, T., Robert, M., Soga, T., Kanai, A., Hirasawa, T., Naba, M., Hirai, K., Hoque, A., Ho, P. Y., Kakazu, Y., Sugawara, K., Igarashi, S., Harada, S., Masuda, T., Sugiyama, N., Togashi, T., Hasegawa, M., Takai, Y., Yugi, K., Arakawa, K., Iwata, N., Toya, Y., Nakayama, Y., Nishioka, T., Shimizu, K., Mori, H., Tomita, M. (2007). Multiple High-Throughput Analyses Monitor the Response of E. coli to Perturbations. Science 316: 593-597 [Abstract] [Full Text]  
  • Keene, J. D. (2007). Biological Clocks and the Coordination Theory of RNA Operons and Regulons. Cold Spring Harb Symp Quant Biol 72: 157-165 [Abstract]  
  • Deutscher, J., Francke, C., Postma, P. W. (2006). How Phosphotransferase System-Related Protein Phosphorylation Regulates Carbohydrate Metabolism in Bacteria. Microbiol. Mol. Biol. Rev. 70: 939-1031 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»