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Microbiology and Molecular Biology Reviews, March 2009, p. 62-70, Vol. 73, No. 1
1092-2172/09/$08.00+0     doi:10.1128/MMBR.00028-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Environmental Proteomics: a Paradigm Shift in Characterizing Microbial Activities at the Molecular Level

Martin Keller1* and Robert Hettich2

Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831,1 Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 378312

Summary: The increase in sequencing capacity led to a new wave of metagenomic projects, enabling and setting the prerequisite for the application of environmental proteomics technologies. This review describes the current status of environmental proteomics. It describes sample preparation as well as the two major technologies applied within this field: two-dimensional electrophoresis-based environmental proteomics and liquid chromatography-mass spectrometry-based environmental proteomics. It also highlights current publications and describes major scientific findings. The review closes with a discussion of critical improvements in the area of integrating experimental mass spectrometry technologies with bioinformatics as well as improved sample handling.

* Corresponding author. Mailing address: Biosciences Division, Oak Ridge National Laboratory, One Bethel Valley Road, P.O. Box 2008, MS-6026, Oak Ridge, TN 37831. Phone: (865) 576-0567. Fax: (865) 574-9223. E-mail: kellerm{at}ornl.gov

Microbiology and Molecular Biology Reviews, March 2009, p. 62-70, Vol. 73, No. 1
1092-2172/09/$08.00+0     doi:10.1128/MMBR.00028-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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