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Microbiology and Molecular Biology Reviews, December 2009, p. 730-749, Vol. 73, No. 4
1092-2172/09/$08.00+0     doi:10.1128/MMBR.00026-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Poxvirus Proteomics and Virus-Host Protein Interactions

Kim Van Vliet,{dagger} Mohamed R. Mohamed,{dagger} Leiliang Zhang,{dagger} Nancy Yaneth Villa, Steven J. Werden, Jia Liu, and Grant McFadden*

Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, Florida

Summary: Studies of the functional proteins encoded by the poxvirus genome provide information about the composition of the virus as well as individual virus-virus protein and virus-host protein interactions, which provides insight into viral pathogenesis and drug discovery. Widely used proteomic techniques to identify and characterize specific protein-protein interactions include yeast two-hybrid studies and coimmunoprecipitations. Recently, various mass spectrometry techniques have been employed to identify viral protein components of larger complexes. These methods, combined with structural studies, can provide new information about the putative functions of viral proteins as well as insights into virus-host interaction dynamics. For viral proteins of unknown function, identification of either viral or host binding partners provides clues about their putative function. In this review, we discuss poxvirus proteomics, including the use of proteomic methodologies to identify viral components and virus-host protein interactions. High-throughput global protein expression studies using protein chip technology as well as new methods for validating putative protein-protein interactions are also discussed.

* Corresponding author. Mailing address: Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, 1600 SW Archer Road, Box 100266, Gainesville, FL 32610. Phone: (352) 273-6852. Fax: (352) 273-8905. E-mail: grantmcf{at}ufl.edu

{dagger} Kim Van Vliet, Mohamed R. Mohamed, and Leiliang Zhang contributed equally to this work.

Microbiology and Molecular Biology Reviews, December 2009, p. 730-749, Vol. 73, No. 4
1092-2172/09/$08.00+0     doi:10.1128/MMBR.00026-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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