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Surviving the Acid Test: Responses of Gram-Positive Bacteria to Low pH

Department of Microbiology and National Food Biotechnology Centre, University College Cork, Cork, Ireland

SUMMARY

INTRODUCTION

MECHANISMS OF RESISTANCE

Proton Pumps

F1F0-ATPase.

(i) Enterococcus hirae.

(ii) F1F0-ATPase in other genera.

Glutamate decarboxylase.

(i) Lactic acid bacteria.

(ii) Listeria monocytogenes.

(iii) Mycobacterium leprae.

(iv) Clostridium perfringens.

Electrogenic transport.

Protection or Repair of Macromolecules

Cell Membrane Changes

Production of Alkali

Production of urease.

Arginine deiminase.

Regulators

Sigma factors.

Two-component signal transduction systems.

Cell Density and Biofilms

Alteration of Metabolic Pathways

ACID RESISTANCE OF SELECTED GRAM-POSITIVE BACTERIA

Listeria monocytogenes

Rhodococcus equi

Mycobacterium spp.

Clostridium perfringens

Staphylococcus aureus

Bacillus cereus

Oral Streptococci

Lactic Acid Bacteria

INDUCTION AT LOW pH

CONCLUSION

REFERENCES

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INTRODUCTION

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Gram-positive bacteria play vital roles in food and health, although the nature of this role can vary greatly. Gram-positive bacteria include generally-regarded-as-safe organisms used in food fermentations, ubiquitous gastric commensals, and potentially beneficial probiotic bacteria. However, gram-positive pathogens can also cause diseases ranging from dental caries to potentially fatal gastrointestinal infections. The acidic environments encountered in food and in the gastrointestinal tract provide a significant survival challenge for these diverse organisms. For example, the preferred delivery vehicles for probiotic cultures are yogurts and fermented milks, both of which present an acid challenge. Subsequently the bacteria will need to survive the highly acidic gastric juice if they are to reach the small intestine in a viable state (113, 140). Hence, acid tolerance is accepted as one of the desirable properties used to select potentially probiotic strains. In striking contrast, the acid tolerance of a pathogen such as Listeria monocytogenes, again assisting survival in the stomach and also in the macrophage phagosome, could be considered a virulence factor. Thus, it is timely to consider the mechanisms used by gram-positive organisms to protect themselves from the challenge posed by low-pH environments such as food and gastric juice, and comment on the strategies by which they can be aided or impeded.

While many excellent reviews have outlined the numerous systems which have been characterized in gram-negative bacteria (7, 196, 215) and, to a lesser extent, in oral streptococci (27, 186), until recently the relative lack of information pertaining to acid resistance systems in gram-positive bacteria has militated against a review of this area. This may reflect the perception that the F₁F₀-ATPase proton pump was solely responsible for the survival of some of these organisms in acidic environments. It is now apparent that a combination of constitutive and inducible strategies which result in the removal of protons (H⁺), alkanization of the external environment, changes in the composition of the cell envelope, production of general shock proteins and chaperones, expression of transcriptional regulators, and responses to changes in cell density can all contribute to survival (Fig. 1). These mechanisms counter the negative impact of a reduction in cytoplasmic pH, which can include loss of activity of the relatively acid-sensitive glycolytic enzymes (which severely affects the ability to produce ATP) and structural damage to the cell membrane and macromolecules such as DNA and proteins.

View larger version (50K): [in this window] [in a new window]   FIG. 1. Graphical presentation of the mechanisms of resistance available to gram-positive bacteria. These have been divided into eight categories, and a number of examples are demonstrated. (i) Proton pumps such as the F1F0ATPase or that utilized by the GAD system bring about an increase in internal pH. (ii) Proton repair involving chaperones, proteases, and heat shock proteins results in the protection of proteins or their degradation if damaged. (iii) DNA damaged as a consequence of a low internal pH can be repaired through the excision of errors or the restarting of stalled replication forks. (iv) The involvement of regulators such as 2CSs and sigma factors can induce minor or global responses. (v) Cell density affects cell-to-cell communication. (vi) Cell envelope alterations can protect cells by changing architecture, composition, stability, and activity. (vii) The production of alkali by the ADI or urease system increases the internal pH of the cell. (viii) Metabolic properties can be altered.

View larger version (39K): [in this window] [in a new window]   FIG. 2. Acidic environments where the survival of bacteria has an impact on health or the economy. While contributory factors are listed, these do not include genes and acid resistance systems associated with individual bacteria.

Further down the alimentary canal, ingested microbes enter the stomach. The stomach acts as a reservoir that controls the rate of entry of material into and, more importantly with respect to this review, the number of bacteria entering the small intestine. Humans secrete approximately 2.5 liters of gastric juice each day, generating a fasting gastric pH of 1.5, which increases to between pH 3.0 and 5.0 during feeding (116). The role of gastric acid is most apparent in patients who are achlorydic, i.e., unable to produce HCl, or have undergone a partial gastrectomy. In these circumstances, the number of aerobes, anaerobes, and coliforms increases so that there is overgrowth of bacteria in the stomach. It has also been observed that neutralization of acid by food or bicarbonate decreases the infectious dose; for example, an increase in the pH of the stomach results in gastric transit of greater numbers of Listeria (44, 63, 64, 198, 205, 258) while gastrectomy and chronic gastritis are risk factors for the development of intestinal tuberculosis (62). Interestingly, preadaptation under conditions similar to those encountered by Mycobacterium avium in its natural habitat allows the organisms to resist the acidic conditions of the stomach (20). For obvious reasons, it is also vital that probiotics be able to transit the stomach despite its low pH (118). Intrinsic resistance to gastric acid is a relatively rare probiotic property, with Lactobacillus acidophilus and bifidobacteria being among the most resistant (34). Gastric survival is also a highly valued property because it overcomes the limitations associated with needing to use food carriers with high buffering potential to aid gastric transit and ensure the delivery of viable cells into the small intestine.

Invasive pathogens also need to survive or avoid additional exposure to acid following phagocytosis by macrophages. Initially the phagosomal pH drops due to the activity of the vacuolar ATPase. Then, following phagosome-lysosome fusion, myleoperoxidase is released into the phagolysosome by the host and catalyzes the formation of potent hypochlorous acid (HOCl) from chloride ions and H₂O₂. The Listeria monocytogenes hemolysin protein responds to the initial drop in pH brought about by the vacuolar ATPases to permeabilize the phagosomal membrane and permit the bacterium to escape prior to phagosome-lysosome fusion (14). Pathogenic mycobacteria alter the phagosomal pH by excluding vacuolar ATPase from the phagosome membrane or inhibiting phagosome-lysosome fusion (197). The latter strategy would also appear to be used by Rhodococcus equi (260).

In this review the mechanisms of acid resistance that are most common among gram-positive bacteria are described. In addition, the consequences of survival of gram-positive bacteria at low pH with the aid of these and other, less common, systems are discussed. We also discuss how this knowledge might be adapted to enhance the survival of beneficial bacteria and reduce the survival of pathogenic bacteria in low-pH environments.

MECHANISMS OF RESISTANCE

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(i) Enterococcus hirae. Initial studies of the importance of the F₁F₀-ATPases in the regulation of cytoplasmic pH were made with Enterococcus hirae (formerly Streptococcus faecalis). This organism does not have a respiratory chain and thus is not capable of using the F₁F₀ complex for synthesis of ATP via oxidative phosphorylation. As a result, the sole function of this complex in E. hirae is the extrusion of hydrogen ions (H⁺) and consequently the establishment of pH homeostasis (111). ATPase activity in E. hirae is controlled primarily at the level of pH-dependent subunit assembly with a decrease in cytoplasmic pH (pH₁), resulting in increased ATPase activity (6, 132, 133). Regulation of transcription or translation does not seem to play an important role. While translation is stimulated at low pH, it occurs at too low a level to account for the increase in the level of membrane-bound ATPase (6). A basal level of ATPase also exists at alkaline pH despite the lack of an obvious role in these conditions (133). It is speculated that it may be present to allow the rapid resumption of growth should the pH drop suddenly (124). In addition, the acid optimum of the complex contributes to pH homeostasis by preventing overalkalization of the cytoplasm (131). The importance of the F₁F₀-ATPase was demonstrated by the isolation of mutants that were sensitive to acid as a result of defects in the complex. Such sensitivity is generally typified by an inability to grow at pH 6, despite growth rates matching those of the parent when grown at pH 7.8 (218, 219).

(ii) F₁F₀-ATPase in other genera. It was found that different acid tolerances in species of oral lactic acid bacteria corresponded to their relative permeabilities to protons. As well as the passive inflow, the permeability of a cell to protons is dictated by an active outflow through the proton-translocating membrane ATPase. Thus, the pHs at which the permeabilities of the relatively acid-tolerant Streptococcus mutans (pH 5) and S. salivarius (pH 6) and the relatively sensitive S. sanguis (pH 7) were lowest are reflected by the pH optima of the ATPase enzymes in these species of pH 6.0, 7.0, and 7.5, respectively. These figures correspond to the relative acid tolerances of these bacteria, indicating the importance of the role played by the ATPase in resistance to low-pH stress (17, 217). The S. sanguis and S. mutans ATPase operons have been compared, but it has not been possible to identify differences that might explain their contrasting activity at low pH (186). It is interesting that the pH profiles of these F₁ subunits are similar, differing dramatically only following binding with F₀ (217). The link between ATPase and acid tolerance has also been demonstrated for ruminal bacteria, where membranes of acid-sensitive bacteria such as Ruminococcus albus and Fibrobacter succinogenes contain much lower levels of ATPase than do those of the relatively tolerant Megasphaera elsdenii and Streptococcus bovis. While the variations in tolerance cannot be explained by the pH optima of the enzymes for these bacteria, since they are all similar, the activity profiles show that the ATPases from the tolerant bacteria are less sensitive to low pH (160), which is also the explanation for the acid resistance of the Leuconostoc oenos mutant LoV8413 (80). In accordance with the above observations, the acid sensitivities of the Lactococcus lactis subsp. lactis C2 mutant (5, 257) and the Lactobacillus helveticus mutant CPN4 (254) are explained by reduced ATPase activity at low pH, and thus the variations in acid tolerance in all of the above examples can be attributed to their relative ATPase activities at low pH.

To date, all streptococci appear to possess an F₀ gene order consisting of atpEBF, in contrast to atpBEF in most other bacteria (Fig. 3). However, the significance of this remains unclear since rearrangement of the genes within the Escherichia coli operon does not appear to influence the assembly and function of the enzyme (246). Interestingly, the S. mutans, S. sanguis, Lactobacillus acidophilus, and Lactococcus lactis operons all lack an atpI homologue upstream of the structural gene. The role of AtpI in the biology of bacterial ATPase is unknown, and although mutations have a slightly detrimental effect, ATPase assembly and function are not impaired. In S. sanguis (137) and S. mutans (213), a relatively large intergenic region with no known role occupies this position. The external pH has no effect on the transcriptional start sites for the ATPase operons in S. mutans (213) or S. sanguis (137), implying that the intergenic region is unlikely to be regulated.

View larger version (48K): [in this window] [in a new window]   FIG. 3. (A) Alignment of ADI gene clusters. arcA, arginine deiminase; arcB, ornithine transcarbamylase; arcC, carbamate kinase; arcR, Crp/Fnr-type regulator; arcD, ornithine/arginine antiporter; arcT, noncharacterized transaminase; argR, arginine of the ArgR-AhrC family. The identities of a number of the genes indicated above remain putative (L. sakei [262, 263], E. faecalis [11], S. gordonii [78], S. pyogenes [74], S. suis [250], and O. oeni [228]). (B) Alignment of F1F0-ATPase operons (C) Alignment of of genes involved in regulation of sigB. (Panel B is based on data in reference 186; Panel C is based on data in reference 247.)

In addition to lactic acid bacteria, the F₁F₀-ATPase complex plays a major role in the acid resistance mechanisms of other gram-positive bacteria. The involvement of the Listeria monocytogenes F₁F₀-ATPase in resistance to acid stress was confirmed when it was found that cells treated with N,N'-dicyclohexylcarbodiimide (DCCD), an inhibitor of ATPase activity, were much more sensitive to exposure to pH 3.0 than were untreated cells. Treatment with DCCD resulted in a reduction in the level of survivors to 1% (stationary phase) or 0.001% (log phase) of that of untreated controls (67). F₁F₀-ATPase also plays a role in the induction of an acid tolerance response (ATR), i.e., the phenomenon whereby the tolerance of an organism to lethal pH increases as a consequence of a prior exposure to a sublethal pH (50). It was found that acid-adapted logarithmic-phase cells treated with DCCD are greatly sensitized to low pH stress. Two-dimensional gel electrophoresis has shown that the b subunit of the L. monocytogenes ATPase is induced by acid stress, and it may also be significant that the atpC ( subunit) and atpD (ß subunit) genes are preceded by boxes associated with PrfA, the positive regulator of virulence genes (97). In M. smegmatis, the delta pH at external pH of 5.0 was dissipated by protonophores and DCCD. These results demonstrate that proton extrusion by the F₁F₀-ATPase plays a key role in maintaining the internal pH near neutral (191).

In addition to the F₁F₀-ATPase, cation transport ATPases such as K⁺-ATPases can contribute to pH homeostasis through the exchange of K⁺ for H⁺. It was found that at pH_o (pH outside the cell) 5.0 the pH_i (pH inside the cell) of glucose-energized S. mutans is 5.5 in the absence of K⁺ but increases to 6.14 in the presence of 25 mmol of K⁺ per liter (66). Similar observations were reported for L. lactis (128) and E. hirae (9, 130).

Glutamate decarboxylase. Almost 60 years ago, it was proposed that amino acid decarboxylases function to control the pH of the bacterial environment by consuming hydrogen ions as part of the decarboxylation reaction (93). Examples of this are the lysine, arginine, and glutamate decarboxylases (GAD), which operate by combining an internalized amino acid (lysine, arginine, or glutamate) with a proton and exchanging the resultant product (cadaverine, agmatine, or -aminobutyrate) for another amino acid substrate. Thus, an extracellular amino acid is converted to an extracellular product, but the consumption of an intracellular proton results in an increase in intracellular pH. Of the three systems mentioned, only the GAD system has been associated with pH control by gram-positive cells.

Among gram-positive bacteria, genetic characterization of GAD systems has been undertaken for L. lactis (202) and L. monocytogenes (51). However, biochemical studies have been used to analyze the corresponding systems in Clostridium (56, 90), some species of Mycobacterium (184), Bacteroides, Fusobacterium, Eubacterium (10), and Lactobacillus brevis (230). According to the most simple model (212), after uptake of glutamate by the specific transporter, cytoplasmic decarboxylation results in the consumption of an intracellular proton. The reaction product, -aminobutyrate (GABA), is exported from the cell via an antiporter. The net result is an increase in the pH of the cytoplasm due to the removal of hydrogen ions and a slight increase in the extracellular pH due to the exchange of extracellular glutamate for the more alkaline GABA. It has also been proposed (202), based on results presented by Higuchi et al. (115) showing that ATP could be generated by glutamate metabolism in lactobacilli, that the GAD system functions to provide metabolic energy by coupling electrogenic antiport and amino acid decarboxylation. It is thought that three successive decarboxylation-antiport cycles create a PMF sufficient for the synthesis of one molecule of ATP via the F₀F₁ ATPase (Fig. 4). The significance of the GAD system is linked to the presence of glutamate in foods. The use of glutamate for food flavor enhancement is a long-established process and can be traced back to the Orient, where seaweeds rich in glutamate were used for this purpose. In modern foods, the sodium salt monosodium L-glutamate monohydrate, usually termed MSG, is by far the most common source, although the potassium, ammonium, and calcium salts are also used (120). Glutamate is also present in a number of food ingredients, while the amino acid itself is used to adjust the acidity of foods. Protein-rich foods such as meat and milk, as well as many vegetables, also have a high content of bound glutamate, while their fermented derivatives have increased levels of free glutamate. If one also considers the antihypertensive effects of foods containing GABA, it is easy to appreciate the importance of glutamate decarboxylation.

View larger version (27K): [in this window] [in a new window]   FIG. 4. Generation of ATP by transport and proton-consuming decarboxylation. The movement of glutamate (Glu2-) ions into the cell, glutamate decarboxylation, and extrusion of GABA ions (GABA-), the movement of malate (Mal2-) ions into the cell, malate decarboxylation, and extrusion of lactate (Lac-) ions (L. lactis), and the movement of citrate (HCit2-) ions into the cell, oxaloacetate decarboxylation, and extrusion of Lac ions (Lac-) (L. lactis) all create an electrogenic potential. These decarboxylation reactions and the consumption of a proton increase the alkalinity of the cytoplasm. Three cycles of decarboxylation and antiport create a PMF sufficient for the synthesis of ATP via the F1F0-ATPase. Ace-, acetate; Oxace2-, oxaloacetate; Pyr, pyruvate.

It was found that the lactococcal gadC and gadB genes were maximally expressed at low pH and at the onset of stationary phase in the presence of NaCl and glutamate and that insertional inactivation of gadB resulted in increased acid sensitvity. The induction by chloride is postulated as a mechanism for survival in the stomach, where the organism encounters a highly acidic, chloride-rich environment (202). It was also proposed that gadCB may be important for lactococcal survival during cheese production. A study of the production of GABA by cheese starters during cheese ripening (164) showed that of 31 colonies isolated from a variety of cheese starters, GABA production was observed in 17. The purified decarboxylase was found to be active in the pH range from 4.0 to 5.5 (optimum at pH 4.7) and to retain 90% activity between 30 and 70°C (166). Analysis of L. lactis subsp. cremoris strains showed a gadB homolog to be present but not to be active as a consequence of a frameshift mutation (165). This may, at least to some extent, explain why L. lactis subsp. lactis and cremoris can be differentiated on the basis of their abilities to respond to acid stress. It has been found that L. lactis subsp. lactis can induce an ATR while L. lactis subsp. cremoris cannot (129). In addition, the sublethal (minimum pH for growth) and lethal levels of acid stress were pH 4.5 and 2.5, respectively, for L. lactis subsp. lactis but higher, at pH 5.0 and 3.0, respectively, for L. lactis subsp. cremoris (129).

A number of strains of lactobacilli also catalyze the conversion of glutamate to GABA (115, 230). Of these, the Lactobacillus brevis GAD was purified and found to have optimal activity at 30°C and pH 4.2 and in the presence of 0.2 M pyridine-HCl, but not to be activated by NaCl (230). In addition, the ability of the GAD system to contribute to ATP synthesis was first demonstrated in Lactobacillus sp. strain E1 (115).

(ii) Listeria monocytogenes. In L. monocytogenes LO28, two decarboxylase-encoding homologs, gadA and gadB, and an antiporter homolog, gadC, have been characterized (51). Indeed, a third decarboxylase and second antiporter homolog have been identified in the genome-sequenced strain EGDe (97). The system plays a role in the acid tolerance of Listeria during both the logarithmic and stationary phases of growth and is also required for the induction of an optimal acid tolerance response. A number of GAD mutants of strain LO28 have been generated. The acid resistance of these strains is in the following order: wild type > gadA > gadB = gadC > gadAB. Elimination of the GAD system results in sensitization to the low pH of the stomach, resulting in more rapid killing in this environment. This sensitivity manifests itself as a dramatic 6-log-unit reduction of the gadAB mutant on exposure to porcine gastric fluid, whereas the parent survives relatively unscathed. It is notable that strains which display reduced GAD activity are more sensitive to gastric fluid. Indeed, the significant strain variation in GAD activity correlates significantly with the observed levels of acid tolerance, suggesting that GAD represents the key mechanism for maintenance of pH homeostasis in L. monocytogenes in this environment (51).

The gadAB mutant was also used to determine the importance of the GAD system in the survival of L. monocytogenes in low-pH foods such as apple, orange, and tomato juices, yoghurts, salad creams, and mayonnaise (53). The greater acid sensitivity of the mutant was apparent even in foods with levels of free glutamate as low as 0.22 mM. It was also found that survival of the wild-type strain, in acidified reconstituted skim milk, diluted to reduce free glutamate levels, improves in response to supplementation with monosodium glutamate. With the exception of the dilute-milk model, no correlation was observed between glutamate concentrations and the levels of survival of the wild-type strain, nor the relative difference between the wild-type and gadAB strains, suggesting that glutamate is present in excess in all instances (53). It would thus seem that at the mildly acidic pHs of the foods tested, the GAD system can function optimally in the presence of the amount of free glutamate naturally present in these foodstuffs.

(iii) Mycobacterium leprae. Mycobacterium leprae GAD is also proposed to play a role in acid resistance, in this case in the intracellular environment in which the bacteria multiply, or to serve in the creation of energy (184). In addition, it was suggested that the presence of GAD activity in M. leprae is a reflection of the association of this organism with nerve tissue. Since glutamic acid is the most abundant amino acid in the nerve tissue and since GABA is an inhibitory neurotransmitter, glutamate decarboxylation may contribute to the impairment of sensation in lepromatous lesions (184).

(iv) Clostridium perfringens. A GAD enzyme purified from Clostridum perfringens was shown to have a pH optimum of 4.7 (56). A rapid and continuous increase of GAD activity during the late logarithmic phase and early stationary phase was shown to correspond to a reduction in the pH of the culture medium to below 6 (55).

Electrogenic transport. A number of other transporter-dependent metabolic pathways have been implicated in acid stress responses. Malolactic fermentation (MLF) is the conversion of the dicarboxylic malic acid to the monocarboxylic lactic acid. MLF plays a major role in the activity of the wine bacteria Oenococcus oeni (199) and has also been identified in Lactobacillus sakei (31), Lactobacillus plantarum (174), and Lactococcus lactis (192). The L-lactate produced is excreted via either a lactate-malate antiporter (L. lactis [182]) or an electrogenic uniport (O. oeni and L. planatum), both of which allow the synthesis of ATP at low pH (Fig. 4). This ATP production is probably the basis for the implication of MLF in O. oeni acid tolerance (229). Furthermore, due to the pK difference of the carboxylic groups of malate and lactate, the external replacement of malate by lactate results in alkalinization of the medium.

In L. lactis subsp. diacetylactis, bacterial growth at low pH is aided by induction of the citrate-lactate antiporter, CitP (95). It is likely that the generation of energy via electrogenic transport and the alkalinization of the medium are responsible for this stimulation of growth (156). The relief of lactate toxicity is thought to be of primary importance in this regard (151).

Chaperonins intervene in numerous stresses for various tasks such as protein folding, renaturation, protection of denatured proteins, and evacuation of damaged proteins. Gene expression and protein levels of the DnaK chaperone are up-regulated in response to acid shock and sustained acidification in S. mutans (121). In E. coli, DnaK increases the stability of UvrA (261), suggesting that at least one of the reasons why the chaperonins play a role in the response to acid shock may be to ensure proper functioning of DNA repair mechanisms.

DnaK is a member of the class I heat shock genes in which regulation involves an inverted repeat (CIRCE element). Transcription of the other member of the CIRCE regulon, GroEL, also increases in response to acid shock. In S. mutans, both groESL and dnaK expression are negatively regulated by HrcA, also encoded within the dnaK operon. An hrcA mutant (SM11) was more sensitive to acid, not being able to lower the environmental pH as effectively as the parental phenotype, although it could mount an ATR. Even then, adapted SM11, cells demonstrated a lower F₁F₀-ATPase activity than did adapted wild-type cells (141). These phenotypes are likely to be due to the altered regulation of chaperone production.

A link between acid stress and chaperones has been demonstrated in a number of other gram-positive bacteria. Acid shock induces 33 proteins, including DnaK, GroEL, and UV-inducible proteins, in L. lactis subsp. lactis strain IL 403 (112). DnaK and GroEL are also induced following acid adaptation of Lactobacillus delbrueckii (145), while GroEL is induced in response to acid stress in Clostridium perfringens (238), S. mutans (249), and Listeria monocytogenes (178, 179).

In addition to the protective role of chaperonins, the HtrA protein is proposed to be involved in proteolysis of abnormal proteins synthesized under stressful conditions. An S. mutans HtrA mutant demonstrated a reduced ability to grow at low pH (76). The Clp ATPases also perform a similar role by targeting misfolded protein for degradation by the ClpP peptidase, which itself is not a member of the Clp ATPase family, in addition to protein reactivation and remodeling activities. The Clp proteins are members of the class III heat shock proteins that are regulated by CtsR. Strains lacking ClpP exhibited reduced growth yield at pH 5.0 (141), again demonstrating the need for removal of abnormal proteins.

The small heat-shock protein, Lo18, of O. oeni is induced after acid, heat, and ethanol stress (105). Upstream of hsp18, the gene encoding Lo18, are found two open reading frames, orfB and orfC, encoding a putative regulator of the LysR family and a protein with homologies to multidrug resistance systems. Heterologous expression of these genes in E. coli significantly enhanced viability under acidic conditions (162).

The importance of the role of the cell membrane is demonstrated by the changes in membrane fatty acid profiles in response to a pH drop. S. mutans strains grown at pH 5 had increased levels of monounsaturated fatty acids and longer-chain fatty acids than did those grown at pH 7. In contrast S. sobrinus 6715, which does not adapt well to pH stress, was less capable of changing its membrane composition (187). Acid-adapted I. monocytogenes was also more tolerant to nisin and other ionophores than was the nonadapted counterpart (67). It is suggested that the increase in the production of the straight-chain fatty acids C_14:0 and C_16:0 as well as the decreased C_18:0 levels associated with acid adaptation may be responsible for this enhanced resistance to bacteriocins as well as, to some extent, the cross-protective effects of acid adaptation in general (235).

The dlt operon is involved in the synthesis of D-alanyl-lipoteichoic acid synthesis. Inactivation of dltC, encoding the D-alanyl carrier protein (Dcp), resulted in the generation of an acid-sensitive S. mutans strain. This mutant could not grow below pH 6.5, and unadapted cells were unable to survive a 3-h exposure in medium buffered at pH 3.5, while a pH of 3.0 was required to kill the wild type in the same period. Following acid adaptation, the level of mutant survival was 3 to 4 orders of magnitude lower than that of wild-type cells. Proton permeability assays revealed that the mutant was more permeable to protons than was the wild type (21). Another S. mutans mutant, with a deficiency in the DagK diacylglycerol kinase, demonstrated increased acid sensitivity. This enzyme is involved in phospholipid metabolism, and therefore membrane architecture and composition is again implicated (255).

A number of other genes have been implicated in contributing to membrane stability and activity at low pH. The acid-sensitive phenotype of a transposon mutant, S. mutans AS17, was found to be due to a partial polar effect on the ffh gene (104). Ffh, the 54-kDa subunit homologue of the eukaryotic signal recognition particle, has also been identified in B. subtilis and E. coli and is involved in protein translocation and membrane biogenesis (149). In S. mutans, ffh is located within the secretion and acid tolerance (sat) operon, ylxM, ffh, satC, satD, and satE, which has an acid-inducible promoter upstream of ylxM (134). This explains the induction of the ffh gene in the parent in response to acid shock (104), although it is the only gene of this operon that is involved in acid tolerance (134). The AS17 mutant is unable to induce an ATR (104), while a subsequently created nonpolar mutant was found to be unable to initiate growth at pH 5.0 (134). The finding that F₁F₀-ATPase levels in the mutant were twofold lower than in the parent strain may at least partially explain its acid sensitivity (104). Another protein, SGP (Streptococcus GTP-binding protein), is associated with the membrane when S. mutans is grown under a variety of stress-inducing conditions including low pH (8), while decreased growth is observed at low pH when sgp antisense RNA is expressed (204). SGP, like its E. coli homolog Era, exhibits properties associated with G-proteins, and while it is essential for normal growth, its function has yet to be determined. Era associates with the inner membrane of E. coli, and it has been suggested that it functions by acting as an interface between energy metabolism and macromolecular synthesis at the ribosome (123).

Ammonia is also known to be produced by some mycobacteria and is thought to play a role in the increase in vacuolar pH brought about by phagocytosed mycobacteria (89). It has been proposed that this trait is responsible for the initial capacity of nonpathogenic mycobacteria to survive in macrophages (136).

Production of urease. Urease is a nickel-containing oligomeric enzyme that catalyzes the hydrolysis of urea to two molecules of ammonia and one molecule of carbon dioxide (161). Levels of 3 to 10 mM urea are found in the saliva of healthy individuals (100), although this can range up to 30 mM in patients with renal disease (177). Urease is produced by a discrete subset of oral bacteria, including S. salivarius, Actinomyces naeslundii, and oral haemophili. Of the oral bacteria, S. salivarius produces the greatest quantities of urease (211). The enzyme has maximal activity at pH 7.0 and retains 80% activity between pH 5 and 8.5 but rapidly loses activity at more extreme acidic pHs, being essentially inactive at pH 4.3 (211). Regulation by the growth pH is the preeminent factor in determining the levels of urease produced. In addition, the glucose concentrations in the medium and the growth rate of the cells have an impact (36). At low pH values (pH 5.5), wild-type urease expression increased approximately 600-fold over that observed in cultures grown at pH 7.0 (38). This was supported by the observation that the level of urease-specific mRNA varied depending on the pH (36). The phosphoenolpyruvate-dependent phosphotransferase system also plays a significant role, as shown by the observation that carbon flow through the system could alleviate the repression of urease (38).

The significance of the urease system was best illustrated when a strain of S. mutans, which does not possess a urease system, was genetically engineered to express the urease genes of S. salivarius. Rats infected which this strain had a dramatically lower incidence and severity of all types of caries than did the controls, showing that alkali generation inhibits caries (42).

The S. salivarius urease operon consists of ureIABCEFGD, with ureC, ureB, and ureA encoding the , ß, and subunits that comprise the apoenzyme. The subunits encoded by ureDEFG function as accessory proteins and assist in the incorporation of nickel ions into the enzyme (37). Two promoters, PureI and PureA, were identified, with the former being regulated by acidic pH and carbohydrate levels (39).

The current model proposes the existence of a repressor that is phosphorylated in the absence of sugar and during growth at neutral pH values. When sugar becomes abundant, the phosphotransferase system would preferentially phosphorylate sugar, leaving the urease repressor unphosphorylated, thereby facilitating PureI-driven synthesis. Repression of PureI was also observed when it was cloned into the nonureolytic S. gordonii DL1, suggesting the involvement of a global regulatory circuit (35).

Arginine deiminase. The ADI system has been identified in a variety of bacteria, including mycoplasmas, halobacteria, Pseudomonas sp., Bacillus spp., and a number of lactic acid and dental bacteria catabolizing arginine to ornithine, ammonia, and CO₂ (59). The impact on humans of bacteria utilizing this system varies greatly. Its presence in oral bacteria is regarded as beneficial. Streptococcus gordonii, S. parasanguis, S. rattus, and S. sanguis (S. sanguinius), some lactobacilli, and a few other oral bacteria, including spirochetes, all utilize either the free form of arginine, which is secreted at concentrations averaging about 50 µM, or arginine in salivary peptides and proteins (236). Despite not seeming to play a role in the ATR, at least not in that of S. sanguis, the system does play a role in the acid resistance of a number of LAB (60). Its contribution to the acid resistance of lactic acid bacteria and, more specifically, to that of some of the less inherently acid resistant (i.e. non-mutans) oral lactic acid bacteria is of great importance. The presence of an ADI system in such bacteria is significant because it permits the survival of desirable, less acidogenic bacteria in plaque in the presence of their more aciduric and acidogenic neighbors (26). While ADI-positive bacteria can utilize arginine in the host diet (193), it has also been shown that in salivary levels of arginine, lysine, or peptides containing these amino acids that may be at least partially responsible for the low caries rates in caries-resistant individuals (236). Among the comparatively few ADI-positive organisms that colonize the mouth, S. gordonii is one of the most abundant on tooth biofilms. It is an early colonizer of teeth and forms a major portion of healthy human supragingival dental plaque. Its establishment may even inhibit subsequent colonization by the highly acidogenic, aciduric species responsible for dental caries (78). The system has three main enzymes: ADI, ornithine transcarbamylase and carbamate kinase, encoded by arcA, arcB, and arcC respectively. The three main enzymes involved in the system appear to be inherently acid tolerant, displaying activity at pH 3.1 and even lower in some species (30). The level to which this system can be induced may reflect its importance. The degree of induction is 48-fold in S. rattus, 1,467-fold in S. sanguis 10904, and over 300-fold in S. gordonii (61). One should note, however, that pH itself does not seem to be a major factor in ADI expression in oral streptococci.

The ADI system is also present in a number of lactic acid bacteria involved in different food fermentations, although its desirability varies. Lactobacillus sakei, used in meat fermentations, is unusual in that it is a facultative heterofermenter that possesses an ADI pathway. A mutant that lacked arginine deiminase activity was tested to determine the importance of the ADI system in L. sakei for meat fermentations. While no difference between the mutant and wild type was apparent, the authors point out that the levels of free arginine in such circumstances can vary greatly depending on the proteolytic activity (263). Of 70 strains of sourdough LAB screened for ADI, ornithine transcarbamylase, and carbamate kinase, only obligate heterofermenters possessed all three. One of these, Lactobacillus sanfranciscensis, was examined in greater detail. In addition to enhancing tolerance to acid stress, the system assisted cell growth, assisted cell survival during storage at 7°C, and favored the production of ornithine, an important precursor of crust aroma compounds (70). Arginine is also one of the major amino acids found in grape juice and wine. While the ADI system protects the LAB involved in MLF of wine (148), there is some concern about the creation of ethyl carbamate (urethane) precursors. Ethyl carbamate is an animal carcinogen that is formed in wine during the nonenzymatic reaction of alcohol with citrulline (228).

The ADI system acts as a virulence factor in Streptococcus pyogenes. S. pyogenes is a serious human pathogen and causes infections leading to acute disorders such as pharyngitis, erysipelas, otitis media, and impetigo and potentially to glomerulonephritis, acute rheumatic fever, and reactive arthritis. A protein originally designated streptococcal acid glycoprotein and originally characterized as being an inhibitor of stimulated human peripheral blood mononuclear cell proliferation (73, 74) was ultimately identified as an ADI. Its role in acid resistance is demonstrated by a massive 9-log-unit difference in survival between a wild-type strain and an isogenic streptococcal acid glycoprotein-negative mutant after 6 h at pH 4 in the presence of 10 mM arginine (72). This difference in acid sensitivity is likely to be responsible for the observation that this mutant demonstrates a reduced ability to enter and initially survive in epithelial cells. Streptococcus suis, which causes a number of diseases in young pigs and is also a cause of meningitis in humans, also possesses an equivalent system, although its role in acid resistance and virulence has yet to be established (250).

In addition to arcA, arcB, and arcC, a number of additional genes play a role in the ADI systems of a variety of organisms (Fig. 3). ArcD, an arginine-ornithine transporter, has been identified in Enterococcus faecalis, Lactobacillus sakei, and Streptococcus gordonii, while ArcT, found in L. sakei and S. gordonii, appears to be a dipeptidase. arcR encodes an activator of the ADI system and is a member of the Crp-Fnr family of regulators. Identification of acid-resistant mutants of Lactococcus lactis found that one such mutant resulted from disruption of a gene that demonstrated sequence similarity to ahrC, which encodes a regulator of arginine synthesis and catabolism in Bacillus subtilis (12). It is suggested that this mutation may have resulted in constitutive expression of arginine-metabolic enzymes in the ADI pathway (190). Similar regulators have been found in E. faecalis, where arginine induces expression of arcABCRD by means of two homologous ArgR/AhrC-type regulators (ArgR1/ArgR2). Transcription of argR1 and argR2 is influenced by glucose and arginine, and it is likely that induction of the system by arginine and repression by glucose is mediated by R1 and R2 (see next paragraph). There are R1- and R2-binding sites within the promoter regions of arcA, argR1, and argR2. These promoters also have ArcR- and CcpA-binding sites (11). Additional regulation in L. lactis subsp. cremoris MG1363 may be provided by the two-component signal transduction system, LlkinA-LlrA. While mutation of either of these components results in an ADI-negative and acid-sensitive phenotype, the precise role of this system has yet to be determined (169).

The ADI systems in LAB associated with cheese fermentations (57), L. sakei (262), oral LAB (78, 153), and E. faecalis (209) are subject to catabolite repression by glucose. In fact, the original observation that one of the major functions of the ADI system was to assist acid tolerance followed the observation that S. faecium and S. sanguis mutants which had defective catabolite repression systems and which could simultaneously degrade both glucose and arginine demonstrated greatly increased acid resistance in the presence of glucose (153). The derepression of these mutants can also be compared to the ability of the ADI system to protect cells that have been in stationary phase for a time when natural derepression occurs (28). In contrast, L. sanfranciscensis (70), O. oeni, and other wine LAB (147) catabolize glucose and arginine concurrently.

Sigma factors. ^B is an alternative "stress" sigma factor that has been identified in L. monocytogenes, B. subtilis, and Staphylococcus aureus and whose function has been compared to those of RpoS/^S in gram-negative bacteria as a consequence of its regulation of a large stress-induced regulon in B. subtilis and S. aureus.

^B was initially identified in B. subtilis as controlling transcription of genes induced at the end of the exponential phase (107, 251). It exerts its influence on a regulon of over 100 csb (for "controlled by sigma B") genes in response to environmental and energy stresses. The title of "stress sigma factor" is apt because B. subtilis ^B mutants display a 50- to 100-fold reduced ability to survive heat (54°C), ethanol (9%), salt (10%), or acid (pH 4.3) shocks as well as freezing, desiccation, and exhaustion of glucose or phosphate (241). In fact, heat shock proteins regulated by ^B constitute the class II heat shock regulon.

RsbW (for "regulator of sigma B") functions as an anti- factor (19). RsbW thus prevents ^B activity from occurring at inappropriate times, and its loss is deleterious to logarithmically growing cells (22). RsbV acts as an "anti-anti- factor" by preventing the formation of an RsbW-^B complex (81). RsbV is itself modulated by the phosphatases RsbP (237) and RsbU (240). The sensing of energy stress is mediated through the PAS domain of RsbP (237) and an /ß hydrolase, RsbQ (23), while environmental stress is sensed through the activation of RsbU by a regulatory cascade that includes the RsbS antagonist, the RsbT kinase, the RsbX phosphatase, the RsbR regulator, and four RsbR paralogues, YkoB, YojH, YqhA, and YtvA (1, 2, 127, 239).

The equivalent S. aureus genes were identified by analysis of a Tn 551 mutant that exhibited a dramatically reduced resistance to methicillin. The genes corresponding to rsbU, rsbV, rsbW, and sigB were found between orf136 and CTorf239, which did not show homology to any known proteins (253). No open reading frames corresponding to the B. subtilis rsbR, rsbS, rsbT, or rsbP genes have been identified (Fig. 3). Strain NCTC 8325, which demonstrates low methicillin resistance, has a truncated rsbU gene that seems to be caused by a frameshift. In the highly methicillin-resistant strain COL, a stretch of an additional 11 bp restores the reading frame. ^B mutations, in strains with an intact rsbU gene, resulted in these strains being deficient in their ability to respond to acid (139) and impaired their ability to induce a stationary-phase ATR (32). It was confirmed that RsbU was indeed the major activator of the ^B response to acid stress when it was shown that expression from the ^B-dependent sarA P3 promoter was greatly reduced at pH 5.5 in the absence of RsbU (176).

The rsbU, rsbV, rsbW, sigB, and rsbX genes have also been identified in L. monocytogenes (15, 247). Again, a ^B-dependent promoter has been identified upstream of rsbV, and this is induced by a drop in pH to 5.3. ^B mutants were found to be extremely acid sensitive, with survival of mutant stationary-phase cells exposed to pH 2.5 being 3.6 log units lower than that of the isogenic parent after 2 h (247). Increased sigB transcription was seen following exposure of exponentially growing cells to acid (15). The percent survival of a stationary-phase culture of this mutant was also found to be 10,000-fold lower than that of the wild type after 3 h at pH 2.5. However, adaptation of this mutant at pH 4.5 improved the survival of the mutant by 1,000-fold, suggesting the existence of both ^B-dependent and -independent pathways (86). The ^B mutant also demonstrates reduced virulence potential, possibly because ^B regulates one of the promoters immediately upstream of PrfA (163).

The Brevibacterium flavum sigB gene, a homolog of which has also been identified in B. lactofermentum (172), is significantly induced under several stress conditions, one of which is acid stress (108). While wild-type B. flavum demonstrates a reduced growth rate at pH 4.4, the growth of a sigB mutant was arrested at this pH (109).

The mycobacterial extracytoplasmic function sigma factors regulate gene expression in response to the extracellular environment. One of these, SigE, influences survival following stress in a number of mycobacteria, as demonstrated by the enhanced rate of killing of an M. smegmatis sigE mutant at pH 4 (252).

Two-component signal transduction systems. Bacteria can also sense and respond to environmental changes through the use of two-component signal transduction systems (2CSs). 2CSs typically consist of a membrane-associated histidine kinase sensor and a cytoplasmic response regulator. 2CSs function in bacterial adaptation, survival, and virulence by sensing environmental parameters and giving the bacteria a mechanism through which they can respond.

A transposon mutant of L. monocytogenes has been identified which is more sensitive to low pH than the wild type during the logarithmic phase of growth and yet more acid resistant during stationary phase. The mutation responsible for this phenotype is located in a two-component system, lisRK (49). This system is also involved in the resistance of Listeria to agents that act at the cell envelope, such as ethanol, nisin, and cephalosporins (49, 52). On the basis of the roles of LisRK homologs in other bacteria (103, 157), as well as its regulation of a penicillin binding protein, it is likely that the growth phase variation in acid sensitivity of this mutant is linked to the regulation of cell envelope composition. The LisRK system is the only one of a number of 2CSs from Listeria that plays a role in acid stress resistance (125). In addition, of a number of PhoP-PhoS homologs identified in E. faecalis only one, EtaRS, a homolog of LisRK, had an impact on the acid resistance of the cell. Mutation of etaR resulted in a 100-fold decrease in the survival of logarithmic cells at pH 3.4 (227).

The role of two 2CSs from S. mutans, ComDE and HK11/RR11, which act in acid resistance and biofilm formation, is described in the next section.

In addition to the acid shocks produced by lactic acid or during sucrose consumption, starvation induces acid resistance of S. mutans in suspension and in biofilms, making it likely that during the "famine" between meals, the bacteria in oral biofilms may be more resistant to acid (259). The ability of S. mutans biofilms to cause dental caries is aided by a number of extracellular sucrose-metabolizing enzymes including three glucosyltransferases, encoded by gtfB, gtfC, and gftfD, and a fructosyltransferase, encoded by the ftf gene. Promoter reporter studies showed that in cells growing as part of a biofilm, the gtfBC genes were induced by sucrose, glucose, and a drop in pH (142).

The induction of acid tolerance at neutral pH has also been the subject of analysis to explain the growth phase acid sensitivity of Lactococcus lactis. "Growth phase acid sensitivity" is the term used to describe the observation that stationary-phase cells inoculated into fresh broth become gradually more acid sensitive, reaching their most sensitive state during the early logarithmic phase before tolerance increases to a maximum at the onset of the stationary phase. The growth phase acid tolerance of L. lactis does not result from changes in internal pH or F₁F₀-ATPase activities, and the increased sensitivity of early-log cells is not related to either glucose levels or lactate production. It was found that cells grown in partially spent TYG (tryptone, yeast extract, glucose) medium had increased relative acid tolerance, but that this enhanced tolerance is eliminated by the addition of tryptone or yeast extract. More specifically, a 1-kDa protease-sensitive putative active compound has been purified and is thought to be responsible for this phenotype (4).

ACID RESISTANCE OF SELECTED GRAM-POSITIVE BACTERIA

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L. monocytogenes can induce a protein synthesis-dependent log-phase ATR when exposed to an induction pH (5.0 to 5.5), dramatically increasing the resistance of log-phase cells (69, 135, 170) to more extreme acidic conditions. One of the primary consequences of acid adaptation appears to be an increased intracellular pH (pH_i), since it was found that at low external pH, acid-adapted and acid-resistant mutant cells maintained an elevated pH_i relative to the wild type. This elevation in pH_i is dependent on protein synthesis (171). The presence or absence of glucose has a large impact on pH. In the presence of glucose, the pH_i drops below pH 7.0 only when the extracellular pH (pH_o) drops below 4. However, in the absence of glucose, a pH_o of 5.5 causes a drop in pH_i. In both cases, a drop in pH_i coincides with a net influx of protons, indicating that membrane transport processes are the main contributors to the process of pH_i homeostasis in L. monocytogenes subjected to acid stress (206).

Two-dimensional analysis of the proteins induced during adaptation has identified 17, 30, and 21 proteins present at increased levels and 17, 8, and 9 present at reduced levels following adaptation with hydrochloric acid or lactic acid or in the nonadapted ATM56 mutant, respectively, in complex broth. In addition, six proteins were identified following HCl adaptation which were not visible on other gels (171). The proteins induced in response to acid induction and acid stress caused by the addition of HCl to minimal medium have also been examined (178, 179). It was found that 47 and 37 proteins were induced by pH 3.5 and 5.5, respectively, and that 23 of these were common (Table 1). These included ATPase-subunit B and GroEL, both of which have been discussed above.

The acid resistance of Listeria influences its survival in foods. In addition to the role of the GAD system described above, the phenomenon of acid adaptation aids the survival of this food pathogen in acidic foods such as cottage cheese, yoghurt, whole-fat cheddar cheese, orange juice, and salad dressing and thus must be taken into consideration when using low pH as a preservative (92). In addition to the pH, the natural flora of the food can have a significant impact. It was found that the presence of the natural microbial flora that may be established in a meat-processing plant in nonacidic (water) washings had a detrimental effect on the ability of L. monocytogenes to subsequently induce an ATR, thus sensitizing it to subsequent exposure to acid. This may be due to L. monocytogenes having to adapt and survive under the conditions created in the water washings by the gram-negative spoilage flora. Since bacterial pathogens seem to be acid sensitized under the stressful conditions prevailing in nonacidic meat washings, it may be possible to use water, steam, or other nonacid treatments with or without low-pH meat decontamination technologies to manipulate the natural flora in a way that may be advantageous to meat safety (200).

In addition to acidic foods, Listeria encounters low-pH stress in vivo. In agreement with results showing the importance of the role of the GAD acid resistance system in the survival of L. monocytogenes in gastric juice, the increased gastric transit of Listeria following an increase in the pH of the stomach demonstrates the role of stomach acid as a primary barrier against listeriosis (44, 63, 205, 258). A neutropenic-mouse model was used to demonstrate that oral administration of sodium bicarbonate shortly before intragastric inoculation significantly increased the severity of the resulting systemic infection by L. monocytogenes strain EGD (64). When the murine gastric pH was lowered through the use of acidified water, it was observed that acid-adapted cells enjoyed increased resistance relative to their unadapted counterparts (198). These results suggest that control of gastric pH through dietary practices or use of inhibitors of gastric acid secretion may be potential aggravating risk factors for food-borne listeriosis.

The induction of an ATR does not improve the survival of L. monocytogenes cells inoculated into the intraperitoneal cavity of mice. However, it has been speculated that this may be a consequence of cells naturally developing acid tolerance following uptake by macrophages, thus eliminating any advantage that would be conferred by prior adaptation (91). Acid-adapted cells do, however, enjoy an advantage over nonadapted cells in cell culture assays, as is evidenced by a ninefold increase in the invasion efficiency of the enterocyte-like cell line Caco-2. This advantage also extends to growth in lipopolysaccharide-treated J774.A1 macrophages. In these cells, four- to fivefold-greater numbers of acid-adapted Listeria were present 4 h postinfection, relative to the number in untreated cells. At 8 h postinfection, the majority of acid-adapted cells were free in the cytoplasm whereas a large number of the nonadapted cells remained in intact phagosomes (46). Both adapted and nonadapted bacteria were phagocytosed and multiplied efficiently in nonactivated THP-1 macrophages. Following activation of the macrophages, the rate of invasion of acid-adapted and nonadapted cells remained equal. However, the active macrophages became nonpermissive to growth of the nonadapted cells while acid-adapted cells demonstrated a greatly enhanced intracellular growth rate (47).

The enhanced survival of acid-adapted L. monocytogenes in activated human macrophages is associated with an altered pattern of expression of genes involved in acid resistance and cell invasion. Transcription of plcA, which is involved in vacuolar escape and cell-to-cell spread, is decreased by 12% after acid adaptation. In contrast, increased transcription of sodC (310%) and the combined gad genes (3000%) was observed after acid adaptation (47). It is interesting that while Hly and PlcA are both expressed in the phagosome and contribute to vacuolar escape, the pH ranges for their enzymatic activities are different (96, 99). Hly expression requires a more acidic pH (4.5 to 6.5) than does PlcA (pH 5.5 to 7.5) and is triggered by the immediate drop in the pH of the macrophage phagosome following phagocytosis (14), with optimal activity in vivo observed at pH 5.94. The key to the survival of the bacteria appears to be their escape before phagosome-lysosome fusion (71). It was found that inhibitors of vacuolar ATPases such as the lipophilic weak base ammonium chloride bafilomycin A₁ or the carboxylic ionophore monensin completely prevent perforation of the vacuole, suggesting that a drop in pH is a prerequisite for phagosomal escape (14, 48). On the basis of these observations, it is speculated that after cell invasion, the acidic environment of the phagosome could prevent plcA expression while enabling Hly to be accumulated in an enzymatically active state. Pore formation by Hly would then raise the phagosomal pH to neutral values, thereby increasing PlcA expression (47). Such tight regulation is essential, since many virulence factors, including listerial hemolysin and phosphatidylinositol-specific phospholipase C (208), are highly antigenic for the host immune response. Thus, both premature and delayed expression are counterproductive to bacterial survival. This was demonstrated when a leucine-to-threonine conversion was generated at position 461 of hemolysin and resulted in a 10-fold increase in activity at neutral pH. As a consequence of premature permeabilization of the host membrane, resulting in host cell death, there was a 100-fold virulence defect in the mouse listeriosis model (98).

To further appreciate the consequences of the emergence of acid-resistant strains, spontaneous acid-tolerant mutants were isolated which were almost as resistant, without acid induction, as adapted wild-type cells and which could be further induced to display even greater levels of survival (46, 170). Compared with wild-type cells, these mutants displayed enhanced acid tolerance during all stages of growth, enjoyed improved survival in acidic foods (92), displayed enhanced invasiveness and growth in enterocyte-like and macrophage-like cell lines, respectively (46), and caused higher rates of mortality in mice (170). Further evidence for the role of the ATR in virulence has also been obtained by the identification of a mutant, ATR-1, that is unable to induce acid tolerance and displays diminished virulence in mice (154). In this case, it w