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Figure 5


FIG. 5. Whole-cell biocatalyst for the synthesis of steroids obtained by autodisplay of Adx. After transport of apo-Adx to the cell surface, dimers were formed spontaneously and the electron transfer activity of Adx was restored by chemical incorporation of the [2Fe-2S] cluster. After the addition of purified AdR and CYP11A1 or CYP11B1, an efficient whole-cell biocatalyst for the synthesis of pregnenolone or corticosterone, respectively, was obtained. The cell envelope of E. coli provided a membrane environment sufficient for both P450 enzymes to be active. OM, outer membrane.





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