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 Previous Article

Microbiol. Mol. Biol. Rev., Mar 1997, 105-120, Vol 61, No. 1
Copyright © 1997, American Society for Microbiology

RNA editing in kinetoplastid protozoa

K Stuart, TE Allen, S Heidmann and SD Seiwert
Seattle Biomedical Research Institute, Washington 98109-1651, USA. kstuart@u.washington.edu

Mitochondrial transcripts in kinetoplastids undergo remarkable posttranscriptional editing by uridylate insertion and deletion. The often dramatic remodeling of pre-mRNA sequences is directed by small guide RNAs (gRNAs) to produce mature mRNAs. In vitro analyses of editing have been used to determine the mechanism of editing and show that editing occurs by a series of enzyme-catalyzed steps. They also show that chimeric gRNA/mRNA molecules are not editing intermediates as proposed but are aberrant end products of editing. The complexes and molecules that catalyze editing are now being identified and characterized. The origin of editing, its developmental regulation which helps control the switching between terminal respiratory systems during the life cycle of trypanosomes, and other areas for future study are discussed.


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  • Cano, M. I. N., Dungan, J. M., Agabian, N., Blackburn, E. H. (1999). Telomerase in kinetoplastid parasitic protozoa. Proc. Natl. Acad. Sci. USA 96: 3616-3621 [Abstract] [Full Text]  
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  • Kobayashi, M., Shinohara, M., Sakoh, C., Kataoka, M., Shimizu, S. (1998). Lactone-ring-cleaving enzyme: Genetic analysis, novel RNA editing, and evolutionary implications. Proc. Natl. Acad. Sci. USA 95: 12787-12792 [Abstract] [Full Text]  
  • Allen, T. E., Heidmann, S., Reed, R., Myler, P. J., Göringer, H. U., Stuart, K. D. (1998). Association of Guide RNA Binding Protein gBP21 with Active RNA Editing Complexes in Trypanosoma brucei. Mol. Cell. Biol. 18: 6014-6022 [Abstract] [Full Text]  
  • Xu, Y.-x., Liu, L., Lopez-Estrano, C., Michaeli, S. (2001). Expression Studies on Clustered Trypanosomatid Box C/D Small Nucleolar RNAs. J. Biol. Chem. 276: 14289-14298 [Abstract] [Full Text]  
  • Horvath, A., Kingan, T. G., Maslov, D. A. (2000). Detection of the Mitochondrially Encoded Cytochrome c Oxidase Subunit I in the Trypanosomatid Protozoan Leishmania tarentolae. EVIDENCE FOR TRANSLATION OF UNEDITED mRNA IN THE KINETOPLAST. J. Biol. Chem. 275: 17160-17165 [Abstract] [Full Text]  


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