MMBR Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pfeifer, B. A.
Right arrow Articles by Khosla, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pfeifer, B. A.
Right arrow Articles by Khosla, C.

 Previous Article  |  Next Article 

Microbiology and Molecular Biology Reviews, March 2001, p. 106-118, Vol. 65, No. 1
1092-2172/01/$04.00+0   DOI: 10.1128/MMBR.65.1.106-118.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Biosynthesis of Polyketides in Heterologous Hosts

Blaine A. Pfeifer1 and Chaitan Khosla1,2,3,*

Departments of Chemical Engineering,1 Chemistry,2 and Biochemistry,3 Stanford University, Stanford, California 94305-5025

Polyketide natural products show great promise as medicinal agents. Typically the products of microbial secondary biosynthesis, polyketides are synthesized by an evolutionarily related but architecturally diverse family of multifunctional enzymes called polyketide synthases. A principal limitation for fundamental biochemical studies of these modular megasynthases, as well as for their applications in biotechnology, is the challenge associated with manipulating the natural microorganism that produces a polyketide of interest. To ameliorate this limitation, over the past decade several genetically amenable microbes have been developed as heterologous hosts for polyketide biosynthesis. Here we review the state of the art as well as the difficulties associated with heterologous polyketide production. In particular, we focus on two model hosts, Streptomyces coelicolor and Escherichia coli. Future directions for this relatively new but growing technological opportunity are also discussed.

* Corresponding author. Mailing address: Department of Chemical Engineering, Stanford University, Stanford, CA 94305-5025. Phone and Fax: (650) 723-6538. E-mail: ck{at}chemeng.stanford.edu.

Microbiology and Molecular Biology Reviews, March 2001, p. 106-118, Vol. 65, No. 1
1092-2172/01/$04.00+0   DOI: 10.1128/MMBR.65.1.106-118.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Appl. Environ. Microbiol. Infect. Immun. Eukaryot. Cell
Mol. Cell. Biol. J. Virol. J. Bacteriol.
ALL ASM JOURNALS

Copyright © 2001 by the American Society for Microbiology. All rights reserved.