This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Barabote, R. D.
Right arrow Articles by Saier, M. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Barabote, R. D.
Right arrow Articles by Saier, M. H., Jr.

 Previous Article  |  Next Article 

Microbiology and Molecular Biology Reviews, December 2005, p. 608-634, Vol. 69, No. 4
1092-2172/05/$08.00+0     doi:10.1128/MMBR.69.4.608-634.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Comparative Genomic Analyses of the Bacterial Phosphotransferase System

Ravi D. Barabote and Milton H. Saier Jr.*

Division of Biological Sciences, University of California at San Diego, La Jolla, California 92093-0116

We report analyses of 202 fully sequenced genomes for homologues of known protein constituents of the bacterial phosphoenolpyruvate-dependent phosphotransferase system (PTS). These included 174 bacterial, 19 archaeal, and 9 eukaryotic genomes. Homologues of PTS proteins were not identified in archaea or eukaryotes, showing that the horizontal transfer of genes encoding PTS proteins has not occurred between the three domains of life. Of the 174 bacterial genomes (136 bacterial species) analyzed, 30 diverse species have no PTS homologues, and 29 species have cytoplasmic PTS phosphoryl transfer protein homologues but lack recognizable PTS permeases. These soluble homologues presumably function in regulation. The remaining 77 species possess all PTS proteins required for the transport and phosphorylation of at least one sugar via the PTS. Up to 3.2% of the genes in a bacterium encode PTS proteins. These homologues were analyzed for family association, range of protein types, domain organization, and organismal distribution. Different strains of a single bacterial species often possess strikingly different complements of PTS proteins. Types of PTS protein domain fusions were analyzed, showing that certain types of domain fusions are common, while others are rare or prohibited. Select PTS proteins were analyzed from different phylogenetic standpoints, showing that PTS protein phylogeny often differs from organismal phylogeny. The results document the frequent gain and loss of PTS protein-encoding genes and suggest that the lateral transfer of these genes within the bacterial domain has played an important role in bacterial evolution. Our studies provide insight into the development of complex multicomponent enzyme systems and lead to predictions regarding the types of protein-protein interactions that promote efficient PTS-mediated phosphoryl transfer.

* Corresponding author. Mailing address: Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093-0116. Phone: (858) 534-4084. Fax: (858) 534-7108. E-mail: msaier{at}ucsd.edu.

Microbiology and Molecular Biology Reviews, December 2005, p. 608-634, Vol. 69, No. 4
1092-2172/05/$08.00+0     doi:10.1128/MMBR.69.4.608-634.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Tessema, G. T., Moretro, T., Kohler, A., Axelsson, L., Naterstad, K. (2009). Complex Phenotypic and Genotypic Responses of Listeria monocytogenes Strains Exposed to the Class IIa Bacteriocin Sakacin P. Appl. Environ. Microbiol. 75: 6973-6980 [Abstract] [Full Text]  
  • Rouquet, G., Porcheron, G., Barra, C., Reperant, M., Chanteloup, N. K., Schouler, C., Gilot, P. (2009). A Metabolic Operon in Extraintestinal Pathogenic Escherichia coli Promotes Fitness under Stressful Conditions and Invasion of Eukaryotic Cells. J. Bacteriol. 191: 4427-4440 [Abstract] [Full Text]  
  • Pan, X., Ge, J., Li, M., Wu, B., Wang, C., Wang, J., Feng, Y., Yin, Z., Zheng, F., Cheng, G., Sun, W., Ji, H., Hu, D., Shi, P., Feng, X., Hao, X., Dong, R., Hu, F., Tang, J. (2009). The Orphan Response Regulator CovR: a Globally Negative Modulator of Virulence in Streptococcus suis Serotype 2. J. Bacteriol. 191: 2601-2612 [Abstract] [Full Text]  
  • Ward, N. L., Challacombe, J. F., Janssen, P. H., Henrissat, B., Coutinho, P. M., Wu, M., Xie, G., Haft, D. H., Sait, M., Badger, J., Barabote, R. D., Bradley, B., Brettin, T. S., Brinkac, L. M., Bruce, D., Creasy, T., Daugherty, S. C., Davidsen, T. M., DeBoy, R. T., Detter, J. C., Dodson, R. J., Durkin, A. S., Ganapathy, A., Gwinn-Giglio, M., Han, C. S., Khouri, H., Kiss, H., Kothari, S. P., Madupu, R., Nelson, K. E., Nelson, W. C., Paulsen, I., Penn, K., Ren, Q., Rosovitz, M. J., Selengut, J. D., Shrivastava, S., Sullivan, S. A., Tapia, R., Thompson, L. S., Watkins, K. L., Yang, Q., Yu, C., Zafar, N., Zhou, L., Kuske, C. R. (2009). Three Genomes from the Phylum Acidobacteria Provide Insight into the Lifestyles of These Microorganisms in Soils. Appl. Environ. Microbiol. 75: 2046-2056 [Abstract] [Full Text]  
  • Taghavi, S., Garafola, C., Monchy, S., Newman, L., Hoffman, A., Weyens, N., Barac, T., Vangronsveld, J., van der Lelie, D. (2009). Genome Survey and Characterization of Endophytic Bacteria Exhibiting a Beneficial Effect on Growth and Development of Poplar Trees. Appl. Environ. Microbiol. 75: 748-757 [Abstract] [Full Text]  
  • Pinedo, C. A., Gage, D. J. (2009). HPrK Regulates Succinate-Mediated Catabolite Repression in the Gram-Negative Symbiont Sinorhizobium meliloti. J. Bacteriol. 191: 298-309 [Abstract] [Full Text]  
  • Shi, Z., Blaschek, H. P. (2008). Transcriptional Analysis of Clostridium beijerinckii NCIMB 8052 and the Hyper-Butanol-Producing Mutant BA101 during the Shift from Acidogenesis to Solventogenesis. Appl. Environ. Microbiol. 74: 7709-7714 [Abstract] [Full Text]  
  • Stoll, R., Mertins, S., Joseph, B., Muller-Altrock, S., Goebel, W. (2008). Modulation of PrfA activity in Listeria monocytogenes upon growth in different culture media. Microbiology 154: 3856-3876 [Abstract] [Full Text]  
  • Riess, T., Dietrich, F., Schmidt, K. V., Kaiser, P. O., Schwarz, H., Schafer, A., Kempf, V. A. J. (2008). Analysis of a Novel Insect Cell Culture Medium-Based Growth Medium for Bartonella Species. Appl. Environ. Microbiol. 74: 5224-5227 [Abstract] [Full Text]  
  • Joseph, B., Mertins, S., Stoll, R., Schar, J., Umesha, K. R., Luo, Q., Muller-Altrock, S., Goebel, W. (2008). Glycerol Metabolism and PrfA Activity in Listeria monocytogenes. J. Bacteriol. 190: 5412-5430 [Abstract] [Full Text]  
  • Sutcliffe, I. C., Black, G. W., Harrington, D. J. (2008). Bioinformatic insights into the biosynthesis of the Group B carbohydrate in Streptococcus agalactiae. Microbiology 154: 1354-1363 [Abstract] [Full Text]  
  • Pfluger, K., de Lorenzo, V. (2008). Evidence of In Vivo Cross Talk between the Nitrogen-Related and Fructose-Related Branches of the Carbohydrate Phosphotransferase System of Pseudomonas putida. J. Bacteriol. 190: 3374-3380 [Abstract] [Full Text]  
  • Pinedo, C. A., Bringhurst, R. M., Gage, D. J. (2008). Sinorhizobium meliloti Mutants Lacking Phosphotransferase System Enzyme HPr or EIIA Are Altered in Diverse Processes, Including Carbon Metabolism, Cobalt Requirements, and Succinoglycan Production. J. Bacteriol. 190: 2947-2956 [Abstract] [Full Text]  
  • Monderer-Rothkoff, G., Amster-Choder, O. (2007). Genetic Dissection of the Divergent Activities of the Multifunctional Membrane Sensor BglF. J. Bacteriol. 189: 8601-8615 [Abstract] [Full Text]  
  • Xue, J., Miller, K. W. (2007). Regulation of the mpt Operon in Listeria innocua by the ManR Protein. Appl. Environ. Microbiol. 73: 5648-5652 [Abstract] [Full Text]  
  • Sebaihia, M., Peck, M. W., Minton, N. P., Thomson, N. R., Holden, M. T.G., Mitchell, W. J., Carter, A. T., Bentley, S. D., Mason, D. R., Crossman, L., Paul, C. J., Ivens, A., Wells-Bennik, M. H.J., Davis, I. J., Cerdeno-Tarraga, A. M., Churcher, C., Quail, M. A., Chillingworth, T., Feltwell, T., Fraser, A., Goodhead, I., Hance, Z., Jagels, K., Larke, N., Maddison, M., Moule, S., Mungall, K., Norbertczak, H., Rabbinowitsch, E., Sanders, M., Simmonds, M., White, B., Whithead, S., Parkhill, J. (2007). Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes. Genome Res 17: 1082-1092 [Abstract] [Full Text]  
  • Pfluger, K., de Lorenzo, V. (2007). Growth-dependent Phosphorylation of the PtsN (EIINtr) Protein of Pseudomonas putida. J. Biol. Chem. 282: 18206-18211 [Abstract] [Full Text]  
  • Velazquez, F., Pfluger, K., Cases, I., De Eugenio, L. I., de Lorenzo, V. (2007). The Phosphotransferase System Formed by PtsP, PtsO, and PtsN Proteins Controls Production of Polyhydroxyalkanoates in Pseudomonas putida. J. Bacteriol. 189: 4529-4533 [Abstract] [Full Text]  
  • Mertins, S., Joseph, B., Goetz, M., Ecke, R., Seidel, G., Sprehe, M., Hillen, W., Goebel, W., Muller-Altrock, S. (2007). Interference of Components of the Phosphoenolpyruvate Phosphotransferase System with the Central Virulence Gene Regulator PrfA of Listeria monocytogenes. J. Bacteriol. 189: 473-490 [Abstract] [Full Text]  
  • Maze, A., O'Connell-Motherway, M., Fitzgerald, G. F., Deutscher, J., van Sinderen, D. (2007). Identification and Characterization of a Fructose Phosphotransferase System in Bifidobacterium breve UCC2003. Appl. Environ. Microbiol. 73: 545-553 [Abstract] [Full Text]  
  • Deutscher, J., Francke, C., Postma, P. W. (2006). How Phosphotransferase System-Related Protein Phosphorylation Regulates Carbohydrate Metabolism in Bacteria. Microbiol. Mol. Biol. Rev. 70: 939-1031 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»