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Microbiology and Molecular Biology Reviews, June 2006, p. 362-439, Vol. 70, No. 2
1092-2172/06/$08.00+0     doi:10.1128/MMBR.00036-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The Escherichia coli Proteome: Past, Present, and Future Prospects{dagger}

Mee-Jung Han1 and Sang Yup Lee1,2*

Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research Center,1 Department of BioSystems and Bioinformatics Research Center, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea2

Proteomics has emerged as an indispensable methodology for large-scale protein analysis in functional genomics. The Escherichia coli proteome has been extensively studied and is well defined in terms of biochemical, biological, and biotechnological data. Even before the entire E. coli proteome was fully elucidated, the largest available data set had been integrated to decipher regulatory circuits and metabolic pathways, providing valuable insights into global cellular physiology and the development of metabolic and cellular engineering strategies. With the recent advent of advanced proteomic technologies, the E. coli proteome has been used for the validation of new technologies and methodologies such as sample prefractionation, protein enrichment, two-dimensional gel electrophoresis, protein detection, mass spectrometry (MS), combinatorial assays with n-dimensional chromatographies and MS, and image analysis software. These important technologies will not only provide a great amount of additional information on the E. coli proteome but also synergistically contribute to other proteomic studies. Here, we review the past development and current status of E. coli proteome research in terms of its biological, biotechnological, and methodological significance and suggest future prospects.

* Corresponding author. Mailing address: Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical & Biomolecular Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea. Phone: 82-42-8693930. Fax: 82-42-8693910. E-mail: leesy{at}kaist.ac.kr.

{dagger} Supplemental material for this article may be found at http://mmbr.asm.org/.

Microbiology and Molecular Biology Reviews, June 2006, p. 362-439, Vol. 70, No. 2
1092-2172/06/$08.00+0     doi:10.1128/MMBR.00036-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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