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Review

Genomics Review of Holocellulose Deconstruction by Aspergilli

Fernando Segato, André R. L. Damásio, Rosymar C. de Lucas, Fabio M. Squina, Rolf A. Prade
Fernando Segato
aDepartment of Microbiology & Molecular Genetics, Oklahoma State University, Stillwater, Oklahoma, USA
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André R. L. Damásio
aDepartment of Microbiology & Molecular Genetics, Oklahoma State University, Stillwater, Oklahoma, USA
bLaboratório Nacional de Ciência e Tecnologia do Bioetanol, Centro Nacional de Pesquisa em Energia e Materiais, Campinas, SP, Brazil
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Rosymar C. de Lucas
aDepartment of Microbiology & Molecular Genetics, Oklahoma State University, Stillwater, Oklahoma, USA
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Fabio M. Squina
bLaboratório Nacional de Ciência e Tecnologia do Bioetanol, Centro Nacional de Pesquisa em Energia e Materiais, Campinas, SP, Brazil
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Rolf A. Prade
aDepartment of Microbiology & Molecular Genetics, Oklahoma State University, Stillwater, Oklahoma, USA
bLaboratório Nacional de Ciência e Tecnologia do Bioetanol, Centro Nacional de Pesquisa em Energia e Materiais, Campinas, SP, Brazil
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DOI: 10.1128/MMBR.00019-14
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SUMMARY

Biomass is constructed of dense recalcitrant polymeric materials: proteins, lignin, and holocellulose, a fraction constituting fibrous cellulose wrapped in hemicellulose-pectin. Bacteria and fungi are abundant in soil and forest floors, actively recycling biomass mainly by extracting sugars from holocellulose degradation. Here we review the genome-wide contents of seven Aspergillus species and unravel hundreds of gene models encoding holocellulose-degrading enzymes. Numerous apparent gene duplications followed functional evolution, grouping similar genes into smaller coherent functional families according to specialized structural features, domain organization, biochemical activity, and genus genome distribution. Aspergilli contain about 37 cellulase gene models, clustered in two mechanistic categories: 27 hydrolyze and 10 oxidize glycosidic bonds. Within the oxidative enzymes, we found two cellobiose dehydrogenases that produce oxygen radicals utilized by eight lytic polysaccharide monooxygenases that oxidize glycosidic linkages, breaking crystalline cellulose chains and making them accessible to hydrolytic enzymes. Among the hydrolases, six cellobiohydrolases with a tunnel-like structural fold embrace single crystalline cellulose chains and cooperate at nonreducing or reducing end termini, splitting off cellobiose. Five endoglucanases group into four structural families and interact randomly and internally with cellulose through an open cleft catalytic domain, and finally, seven extracellular β-glucosidases cleave cellobiose and related oligomers into glucose. Aspergilli contain, on average, 30 hemicellulase and 7 accessory gene models, distributed among 9 distinct functional categories: the backbone-attacking enzymes xylanase, mannosidase, arabinase, and xyloglucanase, the short-side-chain-removing enzymes xylan α-1,2-glucuronidase, arabinofuranosidase, and xylosidase, and the accessory enzymes acetyl xylan and feruloyl esterases.

FOOTNOTES

  • Supplemental material for this article may be found at http://dx.doi.org/10.1128/MMBR.00019-14.

  • Copyright © 2014, American Society for Microbiology. All Rights Reserved.
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Genomics Review of Holocellulose Deconstruction by Aspergilli
Fernando Segato, André R. L. Damásio, Rosymar C. de Lucas, Fabio M. Squina, Rolf A. Prade
Microbiology and Molecular Biology Reviews Nov 2014, 78 (4) 588-613; DOI: 10.1128/MMBR.00019-14

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Genomics Review of Holocellulose Deconstruction by Aspergilli
Fernando Segato, André R. L. Damásio, Rosymar C. de Lucas, Fabio M. Squina, Rolf A. Prade
Microbiology and Molecular Biology Reviews Nov 2014, 78 (4) 588-613; DOI: 10.1128/MMBR.00019-14
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  • Top
  • Article
    • SUMMARY
    • INTRODUCTION
    • HOLOCELLULOSE ENZYME BREAKDOWN SYSTEMS
    • ENZYME STRUCTURE-FUNCTION AND SUBSTRATE RELATIONSHIPS
    • CONCLUSIONS
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
    • Author Bios
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