Table 5.

Summary of yeast general transcriptional repressors

FactorMass (kDa)Gene(s)EssentialCharacteristicsReference(s)
Mot1210MOT1,BUR3YesATP-dependent dissociation of TBP from DNA; functions in both activation and repression of transcription; functionally related to Spt3, TFIIA, and NOT complex14, 90, 105, 299, 375
Ccr4a95CCR4NoComponent of Ccr4-NOT complex; implicated in both transcriptional activation and repression289
Dbf266DBF2NoComponent of Ccr4-NOT complex; implicated in both transcriptional activation and repression289
Caf150POP2,CAF1NoComponent of Ccr4-NOT complex; implicated in both transcriptional activation and repression289
Not1240NOT1, CDC39YesComponent of Ccr4-NOT complex; represses transcription from TATA-less promoters; functionally related to Mot1 and Spt390-92, 289
Not222NOT2,CDC36NoComponent of Ccr4-NOT complex90-92, 289
Not394NOT3NoComponent of Ccr4-NOT complex90-92, 289
Not465NOT4,MOT2, CCL1, SIG1NoComponent of Ccr4-NOT complex90-92, 289
Not5NOT5NoComponent of Ccr4-NOT complex89
Bur174BUR1,SGV1YesCdc28-related protein kinase376
Bur246BUR2No376
Bur4BUR4376
Histone H3 (Bur5)15HHT1, BUR5,SIN2NoNucleosome subunit376
Bur616BUR6, NCB1YesHomologous to mammalian DRAP1/NC2α; associated with Ydr1/Ncb1; blocks TBP association with TFIIA, TFIIB?148, 163, 247, 375
Ydr1/Ncb217YDR1, NCB2YesHomologous to mammalian Dr1/NC2β; associated with Bur6; blocks TBP association with TFIIA, TFIIB?148, 163, 247
Spt411SPT4NoPhysically associated with Spt5; human homolog; functions in chromatin remodeling?188, 300, 472
Spt5116SPT5YesPhysically associated with Spt4; functions in chromatin remodeling?471, 472
Spt6168SPT6, SSN20,CRE2YesFunctionally related to SPT4 andSPT5; might function in either nucleosome assembly or chromatin reorganization31, 85, 338, 470, 471
Sin4111SIN4, TSF3NoComponent of SRB/mediator; functions in both activation and repression of transcription; affects chromatin organization72, 230, 231, 282
Rpd349RPD3, SDI2, SDS6NoHDA; interacts with Sin3; homologous to Hda1, Hos1p, Hos2, and Hos3234, 240, 405, 510, 511
Sin3175SIN3, UME4, RPD1,SDI1, CPE1, SDS16NoComponent of Rpd3 HDA complex234, 453, 504, 511, 521, 522
Ssn6107SSN6, CYC8NoComponent of Ssn6-Tup1 complex; does not bind DNA; recruited to promoter by DNA-repressor complexes; contains 10 TPR repeats243, 425, 490, 534
Tup178TUP1, CYC9, FLK1,ROX4, SFL2, UMR7NoComponent of Ssn6-Tup1 complex; does not bind DNA; recruited to promoter by DNA-repressor complexes; directly interacts with histones H3 and H4; contains 7 WD repeats214, 243, 279, 534
  • a Ccr4 was initially identified as a transcriptional coactivator required for glucose derepression (289).