Table 5.

Summary of yeast general transcriptional repressors

FactorMass (kDa)Gene(s)EssentialCharacteristicsReference(s)
Mot1210 MOT1,BUR3 YesATP-dependent dissociation of TBP from DNA; functions in both activation and repression of transcription; functionally related to Spt3, TFIIA, and NOT complex 14, 90, 105,299, 375
Ccr4a 95 CCR4 NoComponent of Ccr4-NOT complex; implicated in both transcriptional activation and repression 289
Dbf266 DBF2 NoComponent of Ccr4-NOT complex; implicated in both transcriptional activation and repression 289
Caf150 POP2,CAF1 NoComponent of Ccr4-NOT complex; implicated in both transcriptional activation and repression 289
Not1240 NOT1, CDC39 YesComponent of Ccr4-NOT complex; represses transcription from TATA-less promoters; functionally related to Mot1 and Spt3 90-92, 289
Not222 NOT2,CDC36 NoComponent of Ccr4-NOT complex 90-92, 289
Not394 NOT3 NoComponent of Ccr4-NOT complex 90-92, 289
Not465 NOT4,MOT2, CCL1, SIG1 NoComponent of Ccr4-NOT complex 90-92, 289
Not5 NOT5 NoComponent of Ccr4-NOT complex 89
Bur174 BUR1,SGV1 YesCdc28-related protein kinase 376
Bur246 BUR2 No 376
Bur4 BUR4 376
Histone H3 (Bur5)15 HHT1, BUR5,SIN2 NoNucleosome subunit 376
Bur616 BUR6, NCB1 YesHomologous to mammalian DRAP1/NC2α; associated with Ydr1/Ncb1; blocks TBP association with TFIIA, TFIIB? 148, 163, 247, 375
Ydr1/Ncb217 YDR1, NCB2 YesHomologous to mammalian Dr1/NC2β; associated with Bur6; blocks TBP association with TFIIA, TFIIB? 148, 163, 247
Spt411 SPT4 NoPhysically associated with Spt5; human homolog; functions in chromatin remodeling? 188, 300, 472
Spt5116 SPT5 YesPhysically associated with Spt4; functions in chromatin remodeling? 471, 472
Spt6168 SPT6, SSN20,CRE2 YesFunctionally related to SPT4 andSPT5; might function in either nucleosome assembly or chromatin reorganization 31, 85, 338, 470, 471
Sin4111 SIN4, TSF3 NoComponent of SRB/mediator; functions in both activation and repression of transcription; affects chromatin organization 72, 230, 231, 282
Rpd349 RPD3, SDI2, SDS6 NoHDA; interacts with Sin3; homologous to Hda1, Hos1p, Hos2, and Hos3 234, 240, 405, 510, 511
Sin3175 SIN3, UME4, RPD1,SDI1, CPE1, SDS16 NoComponent of Rpd3 HDA complex 234, 453, 504, 511, 521, 522
Ssn6107 SSN6, CYC8 NoComponent of Ssn6-Tup1 complex; does not bind DNA; recruited to promoter by DNA-repressor complexes; contains 10 TPR repeats 243, 425, 490, 534
Tup178 TUP1, CYC9, FLK1,ROX4, SFL2, UMR7 NoComponent of Ssn6-Tup1 complex; does not bind DNA; recruited to promoter by DNA-repressor complexes; directly interacts with histones H3 and H4; contains 7 WD repeats 214, 243, 279, 534
  • a Ccr4 was initially identified as a transcriptional coactivator required for glucose derepression (289).