Table 4.

Enzyme activities, muramic acid, diaminopimelic acid, Bchl, and carotenoid content of membrane fractions purified by sucrose density gradient centrifugationa

Sp. and membrane fractionbActivity (μmol/min/mg of protein)Content (nmol/mg of protein)
NADH-dehydrogenaseNADH oxidaseDAPMuBchlCar
R. thiosulfatophilus
 I0.70.05001.35.4
 II0.260.03001.111.4
 III (pellet)0.010.070.20.10.25.9
E. ramosum
 I0.40.005000.040.64
 II0.170.02001.510.8
 III (pellet)0.0060.0050.20.10.254.6
  • a Abbreviations: Mu, muramic acid; DAP, diaminopimelic acid; Car, carotenoid.

  • b For R. thiosulfatophilus, fraction I was isolated from 1.0 M sucrose layer and fraction II was isolated from 1.5 M sucrose layer. For E. ramosum, fraction I was isolated from 1.0 M sucrose layer and fraction II was isolated from 1.2 M sucrose layer.