TABLE 1.

Key mutations affecting the HOG pathway and their phenotypes

Allele(s)MutationEffectPhenotype (reference)
sln1ΔDeletionPathway overactivationLethal (357)
sln1Different point mutationsDiminished pathway activation, activation of Skn7pSensitivity to high osmolarity (164)
ypd1ΔDeletionPathway overactivationLethal (476)
ssk1ΔDeletionBlock of Sln1 branchSensitivity to high osmolarity in sho1Δ (355)
ssk2ΔN or ssk22ΔNN-terminal truncationPathway overactivationLethal (355)
ssk2Δ ssk22ΔDouble deletionBlock of Sln1 branchSensitivity to high osmolarity in sho1Δ (355)
pbs2ΔDeletionPathway blockSensitivity to high osmolarity (61)
PBS2(EE)S514E and T518EPathway activationLethal (40)
pbs2-389K389M, catalytically inactivePathway blockSensitivity to high osmolarity (498)
pbs2-96P96S, no interaction with Sho1pPathway blockSensitivity to high osmolarity in ssk2Δ ssk22Δ (355)
hog1ΔDeletionPathway blockSensitivity to high osmolarity (61)
hog1Various point mutationsConstitutiveOsmoresistant in a pbs2Δ background (33)
ptc1Δ ptp2ΔDouble deletionPathway overactivationLethal (254)
sho1ΔDeletionBlock of Sho1 branchSensitivity to high osmolarity in an ssk2Δ ssk22Δ mutant (355)
ste11ΔDeletionBlock of Sho1 branchSensitivity to high osmolarity in an ssk2Δ ssk22Δ mutant (355)
ste50ΔDeletionBlock of Sho1 branchSensitivity to high osmolarity in an ssk2Δ ssk22Δ mutant (475)
ste20ΔDeletionPartial block of Sho1 branchSlight sensitivity to high osmolarity in an ssk2Δ ssk22Δ mutant (439)