TABLE 5.

Summary of cellulase adsorption parametersa

Organism, substrate and tempBinding capacityBinding affinityReference
mg/gμmol/gμmol/μmolbLiters/gLiters/μmol
Components
    Cellulomonas fimi (30°C)
        CenA BMCC1443.15.0 × 10−4411.89212
        Cex BMCC1843.65.8 × 10−4331.71
    Thermobifida fusca (50°C)
        E3, Avicel260.46.5 × 10−53.10.2070
        E3, BMCC74111.41.8 × 10−31.50.10
        E4, Avicel310.345.5 × 10−50.850.077
        E4, BMCC8759.71.6 × 10−30.490.044
        E5, Avicel310.671.1 × 10−44.80.22
        E5, BMCC55612.01.9 × 10−32.80.13
    Trichoderma reesei
        CBHI, Avicel (4°C)480.741.2 × 10−40.690.044439
        CBHI, Avicel (25°C)571.11.8 × 10−45.40.28637
        CBHI, Avicel (25°C)150.294.7 × 10−5682
        CBHI, Avicel (50°C)250.487.8 × 10−51.70.0970
        CBHI/BMCC (50°C)2394.67.5 × 10−45.40.2870
        CBHII, Avicel (4°C)280.528.4 × 10−51.00.053439
        CBHII, Avicel (25°C)11.30.243.9 × 10−5682
    Trichoderma viride (30°C)
        ExoI6.60.111.8 × 10−55.00.344
        ExoIII631.01.6 × 10−46.90.43
        EndoI, Avicel1302.54.0 × 10−40.880.04
        EndoIII, Avicel260.457.3 × 10−5120.68
        EndoV, Avicel1101.82.9 × 10−40.890.05
        EndoVI, Avicel4.10.081.3 × 10−53.40.18
Multicomponent mixtures and complexes
    Trichoderma reesei
        Avicel (50°C)921.923.1 × 10−41.040.05641
        Avicel (40°C)561.212.0 × 10−43.210.015508
        Cellulose in pretreated woodc (40°C)811.682.7 × 10−41.820.087506
    C. thermocellum
        Avicel (60°C)d17.50.00831.4 × 10−524651750
        Pretreated wood(60°C)d,e3170.152.5 × 10−5344722
  • a Values in bold are as reported; others are calculated.

  • b Micromole of cellulase/micromole of β-glucosidic bond calculated using 6,173 μmol of β-glucosidic bonds/g of cellulose.

  • c Dilute-acid-pretreated wood prepared at 220°C; an average molecular weight of 48,000 is assumed as in reference 641.

  • d Calculated quantities based on a specific activity of 2.4 μmol/mg/min and a molecular mass of 2.1 × 106 Da.

  • e Dilute-acid-pretreated wood prepared at 220°C.