Table 2.

Compendium of cdc42 mutations

MutationCdc42 testedbPhenotypeReference(s)a
G12VSc, Sp, Dm, HsDominant activated; GTPase defective; GTP bound 210, 336, 390, 642
T17NSp, Dm, HsDominant negative; apo or GDP bound 336,447
T25KHsWild-type response to Cdc24-stimulated GDP dissociation 323
N26DHsWild-type response to Cdc24-stimulated GDP dissociation 323
F28LHsRapid nucleotide exchange; transforms NIH 3T3 cells 327
Y32KHsLoss of Cdc24-stimulated GDP dissociation; reduced binding to Cdc42-GAP, IQGAP1, IQGAP2, and mPAK-3 CRIB domains 309, 310, 323,379
T35ASc, Sp, HsLoss of binding to Cdc24 and CRIB-containing effectors; suppresses G12V and D118A 116,447
F37AHsSlight loss of JNK1 activation; disrupts Rac activation; does not affect actin polymerization or filopodium formation 291
F37EHsLoss of Cdc24-stimulated GDP dissociation 323
D38EHsReduced binding to IQGAP1, IQGAP2, and mPAK-3 CRIB domain 310, 379
Y40CHsReduced binding to CRIB-containing effectors; does not affect actin polymerization and filopodium formation 291
Y40KHsWild-type response to Cdc24-stimulated GDP dissociation 323
V44AScMorphogenesis checkpoint; G2/M delay; reduced binding to Cdc24, Cla4, Gic1, Gic2 116, 475a
T58ASc, Sp ts loss-of-function 391
Q61LSc, Sp, HsDominant activated; GTPase defective; GTP bound 642
D63LHsWild-type response to Cdc24-stimulated GDP dissociation 323
D65KHsWild-type response to Cdc24-stimulated GDP dissociation 323
R66DHsWild-type response to Cdc24-stimulated GDP dissociation 323
R68AHsWild-type response to Cdc24-stimulated GDP dissociation 323
P69AHsWild-type response to Cdc24-stimulated GDP dissociation 323
S71PSc, Sp tsloss-of-function 391
F78LHsWild-type response to Cdc24-stimulated GDP dissociation 323
S86PScLoss of binding to Cdc24; cs dominant negative; suppresses G12V 116
S88DHsSpecifies interaction with p190-GAP 322
S89PSc csdominant negative; suppresses G12V 116
W97RSc, Sp ts (Sc), cs (Sp) loss-of-function 391
Q116KHsLoss of Cdc24-stimulated GDP dissociation 323
I117SScLoss of binding to Cdc24 116
D118ASc, Sp, HsDominant negative (Sc); activated (Sp); sequesters Cdc24 116, 390, 641, 642
T138AScLoss of binding to Cdc24 116
G142SScComponent of cdc42-1t sallele 391
C157RScPartialcs dominant negative 116
L165SScLoss of binding to Cdc24 116
K183-187QScPartial delocalization of protein; suppresses G12V 116
K186RSc tsloss-of-function; higher intrinsic GTPase activity 391
C188SSc, Sp, HsPrenylation defective; delocalized protein; intragenic suppressor of G12V, Q61L, D118A mutations 642,643
  • a This is not a complete set of references for the analysis of these mutations. See Fig. 1 for a map of these mutations on the Cdc42p primary amino acid sequence and the text for additional references.

  • b Sc, S. cerevisiae; Sp, S. pombe; Dm, Drosophila; Hs, H. sapiens.