Table 3.

Adhesins and binding proteins

Adhesin-ligand interactionGeneLocationCommentsReference(s)
Protein-protein
 CR2 (C3d binding proteins)194, 395, 563
  60, 50 kDaHyphal surface (60 kDa), yeast cell membrane? (50 kDa)Isolated whole cell hyphal extracts; binding species differ in glycosylation52, 60, 235, 289, 563
  55–60 kDa (minor 67–68, 20 kDa)HyphaeHyphal extract, Western blot with antiserum to CR2235
  66, 40, 20 kDaYeast cells, hyphaeWestern blot of cell wall extract with antiserum to CR2; 40 kDa ubiquitinated300, 486
  94, 68, 60, 50, 31 kDaHyphaeWestern blot of cell wall extract with antiserum to CR2145
 iC3b binding proteinsYeast cells, hyphaeEpithelial, endothelial cell adhesin25, 26, 115, 146, 162, 176, 194
  130 kDa (also 100, 50 kDa)HyphaeImmunoprecipitation of surface proteins by MAb OKM-1117
  165 kDaINT1?Yeast cellsWestern blot of yeast cell cytosol, membrane, but not cell wall extracts with MAb OKM-1153, 210
  66, 55, 42 kDaHyphaeC3 affinity chromatography of soluble hyphal cell extract; 42 kDa binds C3, C3b, iC3b; 55 kDa binds C3 >C3b, iC3b; 66 kDa no binding4
 Fibrinogen binding proteins34, 358, 548
  mp58 (58 kDa)FBP1Some yeast cells, hyphaeFibrinogen binding; ubiquitinated, collagen-like domains5, 60, 484, 486
  68, 60–62 kDaHyphaeMultifunctional binding; fibrinogen, plastic, laminin, C3d33-35, 550
 Laminin binding proteins
  p37 (37 kDa), 67 kDaYeast cell, hyphal cell wall67 kDa only yeast cell wall; p37 of yeast but not hyphal cells binds laminin; reacts with human high-affinity receptor Ab; p37 ubiquitinated, collagen-like domains296, 484, 486
  68, 62, 60 kDaHyphae > yeast cellsMultifunctional binding: fibrinogen, plastic, laminin, C3d33-35
  Multiple speciesLigand affinity blot of yeast cell wall extract164
 Fibronectin binding proteinDifferences reported for fibronectin binding site222, 248, 253, 257, 380, 407, 466, 507
  62, 72 kDa (60, 105 kDa unreduced)Yeast cells, hyphaeAffinity chromatography gelatin or fibronectin; 60-kDa species reacts with MAb OKM-1; 60-kDa species highly glycosylated; more species identified Ab to human fibronectin receptor256, 259
  Multiple speciesYeast cellsYeast cell wall extract; anti-human fibronectin receptor antibody three proteins 40–50 kDa, polydisperse >143; ligand affinity blot additional proteins164
 Collagen binding proteins249, 255, 259
  62, 72 kDa (60, 105 kDa unreduced)Yeast cells, hyphaeAffinity chromatography gelatin or fibronectin; 60-kDa species reacts with MAb OKM-1259
 Entactin binding proteins
  65, 44, 25 kDaYeast cells, hyphaeLigand affinity blot; RGD dependent and independent293
 Vitronectin binding protein
  30 kDaYeast cellsLigand affinity blot with cell wall extract222, 286, 505
 Ala1pALA1?Confers collagen IV, laminin and fibronectin binding on S. cerevisiae, member agglutinin-like gene family154a
Protein-sugar
 Fucose binding proteinYeast cells, hyphae?Buccal, vaginal epithelial cell adhesin; strain variation37, 53, 92, 93, 345, 544
  >15.7 kDa53, 544
 GlcNAc binding proteinYeast cells, hyphae?Buccal, vaginal epithelial cell adhesin; strain variation37, 53, 92, 93, 345, 544
  190 kDaYeast cellsCell wall extracts of galactose- but not glucose-grown cells; not known if is the same protein in reference345129
Protein-glycosphingolipid
 Fimbrial protein 66 kDa?Yeast cells, hyphae?Epithelial cell adhesin; adhesintope shared with P. aeruginosapili279, 583-586
Protein-presumed protein
 Secreted aspartyl proteinase,
 42–45 kDa
SAP1-9Yeast cells, hyphaeBinding endothelial cells, corneocytes147, 433; Table 1
Protein-plastic
 Plastic binding proteins Binding to many plastics, environmental influences181, 243, 344, 458
  60, 68, 200, >200 kDaHyphae60, 68 kDa may be multifunctional binding fibrinogen, laminin, C3d33, 34, 549
  30 kDaAAF1Yeast cells, hyphaeBind polystyrene and epithelial cells20, 21
Carbohydrate-protein
 Acid labile mannan
  β-1,2 mannotetraoseYeast cells, hyphaeMannose receptor, splenic, lymph node macrophage73, 285
Carbohydrate-unknown
 Acid-stable mannan
  Factor 6Yeast cells, hyphaeBEC adhesion357
  UnknownYeast cells, hyphaeSplenic, lymph node macrophage234