Table 3.

Adhesins and binding proteins

Adhesin-ligand interactionGeneLocationCommentsReference(s)
Protein-protein
 CR2 (C3d binding proteins) 194, 395,563
  60, 50 kDaHyphal surface (60 kDa), yeast cell membrane? (50 kDa)Isolated whole cell hyphal extracts; binding species differ in glycosylation 52, 60, 235, 289,563
  55–60 kDa (minor 67–68, 20 kDa)HyphaeHyphal extract, Western blot with antiserum to CR2 235
  66, 40, 20 kDaYeast cells, hyphaeWestern blot of cell wall extract with antiserum to CR2; 40 kDa ubiquitinated 300, 486
  94, 68, 60, 50, 31 kDaHyphaeWestern blot of cell wall extract with antiserum to CR2 145
 iC3b binding proteinsYeast cells, hyphaeEpithelial, endothelial cell adhesin 25, 26, 115, 146, 162, 176, 194
  130 kDa (also 100, 50 kDa)HyphaeImmunoprecipitation of surface proteins by MAb OKM-1 117
  165 kDa INT1? Yeast cellsWestern blot of yeast cell cytosol, membrane, but not cell wall extracts with MAb OKM-1 153, 210
  66, 55, 42 kDaHyphaeC3 affinity chromatography of soluble hyphal cell extract; 42 kDa binds C3, C3b, iC3b; 55 kDa binds C3 >C3b, iC3b; 66 kDa no binding 4
 Fibrinogen binding proteins 34, 358, 548
  mp58 (58 kDa) FBP1 Some yeast cells, hyphaeFibrinogen binding; ubiquitinated, collagen-like domains 5, 60, 484,486
  68, 60–62 kDaHyphaeMultifunctional binding; fibrinogen, plastic, laminin, C3d 33-35,550
 Laminin binding proteins
  p37 (37 kDa), 67 kDaYeast cell, hyphal cell wall67 kDa only yeast cell wall; p37 of yeast but not hyphal cells binds laminin; reacts with human high-affinity receptor Ab; p37 ubiquitinated, collagen-like domains 296, 484, 486
  68, 62, 60 kDaHyphae > yeast cellsMultifunctional binding: fibrinogen, plastic, laminin, C3d 33-35
  Multiple speciesLigand affinity blot of yeast cell wall extract 164
 Fibronectin binding proteinDifferences reported for fibronectin binding site 222, 248, 253, 257, 380, 407, 466, 507
  62, 72 kDa (60, 105 kDa unreduced)Yeast cells, hyphaeAffinity chromatography gelatin or fibronectin; 60-kDa species reacts with MAb OKM-1; 60-kDa species highly glycosylated; more species identified Ab to human fibronectin receptor 256,259
  Multiple speciesYeast cellsYeast cell wall extract; anti-human fibronectin receptor antibody three proteins 40–50 kDa, polydisperse >143; ligand affinity blot additional proteins 164
 Collagen binding proteins 249, 255, 259
  62, 72 kDa (60, 105 kDa unreduced)Yeast cells, hyphaeAffinity chromatography gelatin or fibronectin; 60-kDa species reacts with MAb OKM-1 259
 Entactin binding proteins
  65, 44, 25 kDaYeast cells, hyphaeLigand affinity blot; RGD dependent and independent 293
 Vitronectin binding protein
  30 kDaYeast cellsLigand affinity blot with cell wall extract 222, 286, 505
 Ala1p ALA1 ?Confers collagen IV, laminin and fibronectin binding on S. cerevisiae, member agglutinin-like gene family 154a
Protein-sugar
 Fucose binding proteinYeast cells, hyphae?Buccal, vaginal epithelial cell adhesin; strain variation 37, 53, 92, 93, 345, 544
  >15.7 kDa 53,544
 GlcNAc binding proteinYeast cells, hyphae?Buccal, vaginal epithelial cell adhesin; strain variation 37, 53, 92, 93, 345, 544
  190 kDaYeast cellsCell wall extracts of galactose- but not glucose-grown cells; not known if is the same protein in reference345 129
Protein-glycosphingolipid
 Fimbrial protein 66 kDa?Yeast cells, hyphae?Epithelial cell adhesin; adhesintope shared with P. aeruginosapili 279, 583-586
Protein-presumed protein
 Secreted aspartyl proteinase,
 42–45 kDa
SAP1-9 Yeast cells, hyphaeBinding endothelial cells, corneocytes 147, 433; Table 1
Protein-plastic
 Plastic binding proteins Binding to many plastics, environmental influences 181, 243, 344, 458
  60, 68, 200, >200 kDaHyphae60, 68 kDa may be multifunctional binding fibrinogen, laminin, C3d 33, 34, 549
  30 kDa AAF1 Yeast cells, hyphaeBind polystyrene and epithelial cells 20, 21
Carbohydrate-protein
 Acid labile mannan
  β-1,2 mannotetraoseYeast cells, hyphaeMannose receptor, splenic, lymph node macrophage 73, 285
Carbohydrate-unknown
 Acid-stable mannan
  Factor 6Yeast cells, hyphaeBEC adhesion 357
  UnknownYeast cells, hyphaeSplenic, lymph node macrophage 234