Table 5.

Features of the In2 region of Tn21

FeatureCoordinatesa
In2 boundaries4040–15038
5′ conserved segment4040–5394
IRi 4040–4064
intI1gene(4242–5255)
Pint−10(5285–5290)
Pint−35(5308–5313)
Pc (P1) −355140–5145
Pc (P1) −105163–5168
P2 −355259–5264
3G insertion that creates P25278–5280
P2 −105282–5287
attI1 site5362–5400
Core site, GTTRRRY gene cassette5394–5400
aadA1 gene cassette5395–6250
5′ portion of 59-be5395–5400
aadA1 gene5416–6195
alternate start, aadA1 gene5404
78% identical to merO 5491–5508
3′ portion of 59-be6197–6250
Inverse core site, RYYYAAC6197–6203
Central IR in 59-be6209–6225
Central IR in 59-be6229–6243
Core site, GTTRRRY6250–6256
3′ conserved segment6251–8275
qacEΔ1gene6359–6706
Sequence diverges fromqacE 6650–6651
sul1gene6700–7539
Potential 59-be7534–7567
orf5 gene7667–8167
IS1326boundaries8276–12360
IS1326IR8276–8301
istB gene(8343–9128)
istA gene(9115–10638)
istAB−10(10711–10716)
IS1353 boundaries10720–12332
IS1353 IR10720–10731
orfAB −3510738–10743
orfAB −1010762–10767
orfA gene10791–11429
Frame shift11396–11402
Stem-loop11407–11462
orfB gene11390–12304
IS1353 IR12320–12332
IS1326 IR12335–12360
istAB−35(12348–12353)
tni region(12355–14990)
tniBΔ1 gene(12355–13215)
tniAgene(13218–14933)
tniAB −10(14963–14968)
tniAB −35(14985–14990)
IRt 15014–15038
5-bp direct repeat of target15039–15043
  • a Parentheses indicate that the ORF or control site is on the complementary strand in the sequence compiled in GenBank accession no. AF071413.