Table 7.

Sites of hydrolytic cleavage of Nod factors ofRhizobium meliloti by enzymes isolated from the roots ofMedicago sativaa

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Hydrolase (reference)No. of N-acetylglucosamine residuesR1R2Cleavage site(s)
Intact roots (258,260)IVSulfatylHa
IVHHa, b
IVSulfatyl O-Acetyla
VSulfatylHc, d
VHHc, d, e
Chitinase class I (241,258)IVSulfatylHNone
IVHHb
IVSulfatyl O-AcetylNone
VSulfatylHd
VHHd, e
Novel chitinase/lysozyme (179)IVSulfatylHb
IVHHb
IVSulfatyl O-Acetylb
VSulfatylHd
Nod factor hydrolase (258,260)IVSulfatylHa
IVHHa
IVSulfatyl O-Acetyla
VSulfatylHc
VHHc
VSulfatyl O-Acetylc
  • a Distinct cleavage site preferences exist toward tetrameric (IV) and pentameric (V) Nod factors of R. meliloti as well as their desulfated and deacetylated derivatives, respectively.