Table 3.

Predicted twin-arginine signal peptidesa

Table 3.
  • a Amino-terminal signal peptides were identified as described in the text. Conserved residues of the RR-motif are indicated in bold letters. Only signals containing, in addition to the twin arginines, at least one other residue of the consensus sequence (R-R-X-#-#) (60) were included in this comparison. Positively charged residues in the C-domain that could function as a so-called Sec avoidance motif (23) are indicated in bold and italics. The hydrophobic H-domain is indicated in gray shading. In signal peptides with a predicted SPase I cleavage site, residues from positions −3 to −1 relative to the SPase I cleavage site are underlined, and this site is indicated with a gap in the amino acid sequence. Proteins containing additional cell wall retention signals in the mature part of the protein are indicated with a superscript W; proteins lacking a (putative) type I cleavage site, some of which contain additional transmembrane domains in their “mature” part, are indicated with a superscript TM.