TABLE 1.

Acid-induced proteins identified by using high-throughput screening strategies

Organism (conditions) and genePredicted protein
S. oralis (pH 5.2, 2D-PAGE) (248)60-kDa chaperonin
Hsp33 protein (redox-regulated chaperone)
Cell division protein DivIV A
Superoxide dismutase
Formate acetyitransferase 1
Pyruvate oxidase
NADP-specific glutamate dehydrogenase
Glyceraldehyde-3-phosphate dehydrogenase
l-Lactate dehydrogenase
Fructose bisphosphate aldolase
Acetoin utilization AcuB protein
Galactose-6-phosphate isomerase, LacB subunit
ATP synthase alpha chain
ATP synthase beta chain
ATP synthase gamma chain
ATP synthase delta chain
ATP synthase epsilon chain
Formate-tetrahydrofolate ligase
Oxygen-insensitive NAD(P)H
Nitroreductase/dihydropteridine reductase
ABC transporter
Hypothetical ABC transporter
ATP-binding protein
EIIA component of MTL phosphotransferase system
Nicotinate phosphoribosyltransferase
Adenylate kinase
ReoA protein
Polyribonucleotide nucleotidyltransferase
Polypeptide deformylase
Membrane protein precursor
Succinyl-diaminopimelate desuccinylase
Phosphoprotein phosphatase 2C
M. tuberculosis (pH 5.5, microarray) (88)
    Rv3083Probable monooxygenase
    Rv30084 (lipR)Probable acetyl hydrolase
    Rv3085Short-chain ADH
    Rv3086 (adhD)Zinc-containing ADH
    Rv3087Conserved hypothetical protein
    Rv3088Conserved hypothetical protein
    Rv3089 (fadD13)Acyl-CoA synthase
    Rv0467Isocitrate lyase
    Rv0468 (icl)3-Hydroxyacyl-CoA dehydrogenase
    Rv3614c (fadB2)Conserved hypothetical protein
    Rv3615cConserved hypothetical protein
    Rv3616cConserved hypothetical protein
    Rv1130Conserved hypothetical protein
    Rv1131 (gltA1)Citrate synthase 3
    Rv2428 (ahpC)Alkyl hydroperoxide reductase
    Rv2429 (ahpD)Member of AhpC family
    Rv1245CPutative dehydrogenase
    Rv2007c (fdxA)Ferredoxin
    Rv1404Transcriptional regulator (MarR family)
    MT0196Hypothetical protein
Rv1169c (PE)PE family protein
Rv0582Hypothetical protein
Rv2450c (rpfE)Conserved hypothetical protein (resuscitation-promoting factor)
L. monocytogenes (pH 3.5 and 5.5, 2D-PAGE) (179)ATP synthase (subunit B)
Thoredoxin reductase homologue
Chaperonin
Sigma H factor
Ferric uptake regulator
Alcohol dehydrogenase
YfiV, similar to transcriptional regulator
Similar to transcriptional regulator
Transcriptional regulator
Similar to GroEL
S. mutans (pH 5.2, 2D-PAGE) (249)DnaK
GroEL
Trigger factor, Ppiase
Cell division FtsZ
Cell division protein FtsA
Superoxide dismutase (Mn)
Neutral endopeptidase
Glucan-1,6-α-glucosidase
Phosphoglucomutase/ phosphomannomutase
Enolase
NADP-specific glutamate dehydrogenase
l-Lactate dehydrogenase
Enoyl-(acyl-carrier protein) reductase (NADH)
Fructose bisphosphate aldolase
Acetoin reductase
Metal-dependent hydrolase
Lactoylglutathione lyase
Phosphoenolpyruvate:protein phosphotransferase
Multiple sugar-binding transport protein ATP-binding transport protein MsmK
EF-G
EF-Tu
Small-subunit ribosomal protein S1P
EF-Ts
DNA-directed RNA polymerase alpha chain
Ribosome-recycling factor
S. mutans (pH 5.5, differential-display PCR) (40)
    AP11Hippurate hydrolase
    AP-712Acetolactate synthase, large subunit
    AP-711Pyruvate carboxylase subunit B
    GSP781Putative secreted protein (S. mutans)
    AP-785Conserved hypothetical protein (Thermotoga maritima)
    AP-185Branched-chain amino acid transferase
    AR-186Aconitase hydratase
    AR-731Isocitrate dehydrogenase
    AR-732Citrate synthase