TABLE 29.

Glycolysis, the pentose phosphate cycle, alcoholic fermentation, and gluconeogenesis

EnzymeEC no.NCU no.BLAST match
Best overallS. cerevisiaeS. pombeAnimalaPlantb
Hexose phosphorylation
    Glycogen phosphorylase (GP)2.4.1.107027.1A. fumigatus GP; 0.00Gph1p; 0.00NoneLiver GP; 0.00Os α-1,4-glucan phosphor- ylase; 0.00
    Hexokinase (HK)2.7.1.102542.1A. oryzae HxkA; 1.00e-167Hxk2p; 2.00e-117HK 1; 3.00e-112Dm HK-t2; 1.00e-63Os HK II; 8.00e-54
    Hexokinase-like06996.1A. nidulans XprF; 1.00e-100Glk1p; 1.00e-17HK 1; 6.00e-20Hs MGC:29452; 5.00e-20Os putative HK 1; 4.00e-22
    Hexokinase-like04728.1K. lactis RAG5 hexokinase; 4.00e-34Hxk2p; 2.00e-31HK 1; 7.00e-32Dm HK-t2; 5.00e-26At HK; 2.00e-25
    Glucokinase (GK)2.7.1.200575.1A. niger GK; 1.00e-155Glk1p; 3.00e-77HK 2; 1.00e-76Dm HK-t2; 1.00e-51Os putative HK 1; 3.00e-60
EM glycolysis
    Phosphoglucomutase (PGM)5.4.2.210058.1A. oryzae PgmA; 0.00Pgm2p/Gal5 p; 0.00NP_59615 3.1; 0.00Hs PGM1; 1.00e-158At PGM; 1.00e-155
    Glucose-6-phosphate isomerase (GPI)c5.3.1.907281.1A. oryzae PgiA; 0.00Pgi1p; 0.00NP_59663 5.1; 0.00Dm AAA6367 2.1; 1.00e-168At BAB1763 7.1; 1.00e-111
    6-Phosphofructokinase (PFK)2.7.1.1100629.1A. niger PFK; 0.00Pfk2p; 0.00NP_59594 6.1; 0.00Hs 6-PFK; 0.00At BAB5549 9.1; 1.00e-10
    Fructose-bisphosphate aldolase (FBA)c4.1.2.1307807.1P. brasiliensis AAL34519.2; 1.00e-135Fbalp; 1.00e-128FBA NP_59569 2.1; 1.00e-118Ag EAA0269 2.1; 5.00E-88 (not in mammals)AT T10O22.2 4; 2.00e-08
    Triose-phosphate isomerase (TPI)c5.3.1.107550.1P. brasiliensis TPI; 2.00e-83Tpi1p; 5.00e-79TPI; 7.00e-72Hs TPI 1; 2.00e-77Os TPI; 3.00e-76
    Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)c1.2.1.1201528.1S. macrospora Gpd; 1.00e-178Tdh1p; 1.00e-119GAPDH; 1.00e-127Mm NP_03211 0.1; 2.00e-135At NP_17807 1.1; 1.00e-136
    Phosphoglycerate kinase (PGK)c2.7.2.307914.1T. viride PGK; 0.00Pgk1p; 1.00e-154NP_59673 0.1; 8.00e-163Hs PGK1; 1.00e-160At NP_17601 5.1; 1.00e-105
    Enolase (ENO)c4.2.1.1110042.1A. oryzae ENO; 0.00Eno1p; 1.00e-173NP_59590 3.1; 1.00e-155Dm NP_72272 1.1; 2.00e-149At ENO F2P9.10; 1.00e-140
    Enolasec4.2.1.1101870.1S. japonicum ENO; 8.00e-129Eno1p; 1.00e-120NP_59590 3.1; 1.00e-120Mm ENO 1; 1.00e-123At ENO F2P9.10; 1.00e-122
    Pyruvate kinase (PYK)2.7.1.4006075.1T. reesei PYK; 0.00Pyk1p; 4.00e-173PYK NP_59434 6.1; 9.00e-179Ag EAA1055 5.2; 2.00e-140At PYK NP_19436 9.1; 4.00e-107
HM glycolysis/pentose phosphate pathway
    Glucose-6-phosphate 1-dehydrogenase (GPDH)1.1.1.4909111.1A. niger GPDH; 0.00Zwf1p; 1.00E-160Zwf1; 1.00E-147GPDH X-linked; 1.00e-138At GPDH; 1.00e-132
    6-Phosphogluconol aconase (PGL)3.1.1.3100087.1S. pombe Sol1; 8.00e-65Sol1p; 4.00e-57Top hitMm PGL; 4.00e-42At PGL-like; 4.00e-39
    6-Phosphogluconic dehydrogenase (PGD)1.1.1.4403100.1A. niger PGD; 0.00Gnd1p; 0.00PGD; 0.00Hs PGD protein; 0.00At PGD; 1.00e-115
    6-Phosphogluconic dehydrogenase (PGD)1.1.1.4400837.1A. niger PGD; 1.00e-120Gnd2p; 2.00e-66SPBC660 16; 5.00e-67Mm PGD; 1.00e-62At PGD-related; 9.00e-72
    Ribulose-phosphate 3-epimerase (RPE)5.1.3.100519.1S. cerevisiae Rpe1p; 8.00e-51Top hitSPAC31G 5.05c; 5.00e-48Hs MGC:226 37; 9.00e-44RPE; 9.00e-48
    Transketolase (TK)2.2.1.101328.1A. niger TK; 0.00Tk11p; 0.00SPBC2G5.05; 0.00Hs TK; 2.00e-42At TK precursor related; 0.00
    Transaldolase (TA)2.2.1.202136.1S. kluyveri Tal1p; 1.00e-106Tal1p; 3.00e-95Tal1; 1.00e-106Ag XP_32071 5.1; 2.00e-88At ToTAL2; 7.00e-32
    Transaldolase?2.2.1.206142.1H. sapiens transaldolase; 1.00e-15Tal1p; 5.00e-10Tal1; 2.00e-09Top hitNone
Fermentation
    Pyruvate decarboxylase (PDC)4.1.1.102193.1S. pombe SPAC186.09; 0.00Pdc6p; 6.00e-57Top hitDm CG11208-PA; 2.00E-11At Pdc1; 1.00e-119
    Pyruvate decarboxylase (PDC)4.1.1.102397.1P. anserina CAD60727.1; 0.00Pdc1p; 1.00e-141C13A11.06; 1.00e-163Ce 2-hydroxyphytanoyl-CoA lyase; 1.00e-11At PDC-related protein; 2.00e-63
    Alcohol dehydrogenase (ADH)1.1.1.101754.1A. flavus Adh1; 1.00e-133Adh3p; 1.00e-105Adh1; 9.00e-97Ce probable ADH; 1.00e-73Os CAD3990 7.1; 3.00e-26
    Alcohol dehydrogenase (ADH)1.1.1.102476.1N. crassa NCU01754.1; 1.00e-126Adh3p; 6.00e-97Adh1; 1.00e-87Ce ADH; 2.00e-70At cinnamyl-ADH; 8.00e-30
Gluconeogenesis
    Fructose-bisphosphatase (FBP)3.1.3.1104797.1A. nidulans FBP; 5.00e-163Fbp1p; 1.00e-112P09202; 1.00e-102Dm CG31692-PA; 1.00e-83At 1g43670; 6.00e-93
    Phosphoenolpyruvate carboxykinase (PEPCK)4.1.1.4909873.1A. nidulans PEPCK; 0.00Pck1p; 0.00NoneNoneAt 4g37870; 0.00
  • a Caenorhabditis elegans (Ce), Drosophila melanogaster (Dm), Anopheles gambiae (Ag), Mus musculus (Mm), or Homo sapiens (Hs).

  • b Arabidopsis thaliana (At) or Oryza sativa (Os).

  • c Also a component of gluconeogenic pathway.