TABLE 49.

Known and predicted N. crassa proteins associated with circadian clocks and photobiology

Protein classNeurospora proteinFunctionNCU no.BLAST match
Best overallS. cerevisiaeS. pombeAnimalaPlantb
Clock-associated genesFRQN. crassa central clock component02265.1Sordaria fimicola; e 0.0NoneNoneNoneNone
WC-1N. crassa blue-light photoreceptor, central oscillator component, 2 PAS domains, 1 LOV domain, GATA zinc finger domain02356.1Podospora anserina; e 0.0Ymr1369; e-06SPCC1902.01; 5e-05Drosophila; e-14NP851210.1; 4e-18
WC-2Forms complexes with WC-1, central oscillator component and blue-light signaling, 1 PAS domain, GATA zinc finger domain00902.1Fusarium solani; e-138Ymr136W; 6e-08NoneNoneAC026875.23; 3e-07
CKAN. crassa casein kinase II catalytic subunit involved in FRQ03124.1Candida albicans; e-148YorR061W CKA2; e-106SPAC23C11 CKII; e-130P28020; e-105AF370308; e-112
CKB1N. crassa casein kinase II regulatory subunit involved in FRQ phosphorylation05485.1S. pombe; 4e-61Yg109W CKB1; e-53SPBC2G5.02C CKII; 4e-61P28021; 3e-46AF239816; 4e-41
CKB2N. crassa casein kinase II regulatory subunit involved in FRQ phosphorylation02754.1S. pombe; 2e-77Y1r039C RIC1; 8e-4SPAC1851.04e; 2e-77XP317865.1; 2e-60NP181996.1; 1e-58
HHP1N. crassa casein kinase I involved in FRQ phosphorylation00685.1S. pombe; e-150NP015120.1; e-125NP595760.1; e-150BAB0347 3.1; e-135BAB92346.1; e-130
CAMKN. crassa protein kinase involved in FRQ phosphorylation09123.1Aspergillus nidulans; 1e-165Yoi016C CMK2; e-99SPACUNK12.02c; 4e-67NP065130.1; 2e-69NP192383.1; 8e-49
SHAGGY/GSK-3 homologueDrosophila protein kinase Shaggy involved in phosphorylation and nuclear movement of the central oscillator component TIM04185.1Colletotrichum gloeo- sporioides; e-157Ymi139W Rim11; e-104SPAC1687.15; e-148NP571465; e-130AF43225.1; e-119
FWD-1E3 ubiquitin ligase involed in targeting FRQ for proteasomal turnover04540.1Homo sapiens; e-47NoneSPAC30.05; e-40Homo sapiens; e-47None
Other light- sensing genesVVDN. crassa blue-light sensing, PAS/LOV domain03967.1Aspergillus nidulans; 2e-41NoneNoneNoneBAA84780.1; 3e-19
PHY-1Putative phytochrome red/far-red-light sensing04834.1Pseudomonas putida; 8e-61Ylu206W; 3e-09SPAC27E2.09; 2e-27NoneP55004; 4e-33
PHY-2Putative phytochrome red/far-red-light sensing05790.1Agrobacterium tumefaciens; 2e-33Yi1147C SLN1; 8e-9SPAC1834.08; 2e-14NoneP55004; 2e-21
CRY homologPutative blue-light sensing, central oscillator component in mammals, light entrainment of the clock in Drosophila00582.1Trichodesmium erythraeum; 4e-69Yoi386w PHR1; 4e-26NoneNoneNP58461.2; 2e-49
VELVET homologueRed- and blue-light sensing in Aspergillus01731.1Aspergillus nidulans; 5e-40NoneNoneNoneNone
NOP-1Neurospora homologue of bacteriorhodopsin10055.1Leptosphaeria maculans opsin; 2e-75Yro2p; e-08SPCC31H12.02c; 9e-07NoneNone
ORP-1Neurospora opsin-related protein, lacks conserved lysine residue01735.1Coriolus versicolor Hsp30; 5e-31Yro2p; 8e-23SPCC31H12.02c; 8e-12NoneNone
Other PAS proteinsHypothetical protein4 PAS domains, sensory box histidine kinase/response regulator03164.1Shewanella oneidensis; 6e-31Yi1147C SLN1; 2e-10SPAC27E2.09; 1e-20NoneNone
Predicted protein3 PAS domains06390.1Caenorhabditis elegans; e1.4NoneNoneNoneNone
PAS/PAC domain proteinsHypothetical proteinPAS/PAC domain 1e-88, sensory transduction histidine kinase02057.1Magnetococcus sp. strain MC-1; 4e-75Yi1147C SLN1; 4e-18SPAC183 4.08; 6e-57NoneNone
Hypothetical proteinPAS/PAC domain 3e-59, two-component hybrid sensor and regulator00939.1Anabaena sp. strain PCC 7120; 4e-23Yi1147C SLN1; 6e-11SPAC27E2.09; 2e-25NoneNone
Hypothetical proteinPAS/PAC domain 3e-63, putative two-component histidine kinase Fos-107221.1Aspergillus fumigatus; e-161Yi1147C SLN1; 7e-17SPAC27E2.09; 3e-36NoneNone
Hypothetical proteinPAS/PAC domain 3e-88, related to two-component histidine kinase chk-101833.1Glomerella cingulata; 0.0Ylu206W; e-10SPAC1834.08; 8e-79NoneNone
Hypothetical proteinPAS/PAC domain 5e-42, probable serine/threonine protein kinase, related to plant NPH-1 protein07268.1Caulobacter crescentus; 2e-07NoneNoneNoneT013535; e-07

  • ↵a Caenorhabditis elegans, Rattus norvegicus, Xenopus laevis, Drosophila melanogaster, Anopheles gambiae, Mus musculus, or Homo sapiens.

  • ↵b Arabidopsis thaliana, Zea mays, Ipomoea nil, or Oryza sativa.