TABLE 1.

Functionally characterized autotransporters

ProteinAccession no.Bacterial speciesMol wtSignificant functional motifsKnown functionReference
Cluster 1
    Ssp P09489 S. marcescens 112,345Peptidase S8A subtilisin-like serine protease, RGD cell attachment sequenceProtease 537
    Ssp-h1 BAA33455 S. marcescens 107,598Peptidase S8A subtilisin-like serine protease, RGD cell attachment sequenceNone 360
    Ssp-h2 BAA11383 S. marcescens 107,766Peptidase S8A subtilisin-like serine protease, RGD cell attachment sequenceNone 360
    PspA BAA36466 P. fluorescens 102,684Peptidase S8A subtilisin-like serine protease, RGD cell attachment sequence, P-loop ATP/GTP-binding site motifNone 236
    PspB BAA36467 P. fluorescens 106,789Peptidase S8A subtilisin-like serine protease, P-loop ATP/GTP-binding site motifNone 236
    Ssa1 AAA80490 P. haemolytica 103,585Peptidase S8A subtilisin-like serine proteaseNone 169
    SphB1 CAC44081 B. pertussis 109,801Peptidase S8A subtilisin-like serine proteaseProteolytic processing of secreted proteins 82
    AspA/NalP AAN71715 N. meningitidis 113,640Peptidase S8A subtilisin-like serine protease, P-loop ATP/GTP-binding site motif, prokaryotic membrane lipoprotein lipid attachment siteProteolytic processing of secreted proteins 508
Cluster 2
    VacA Q48247 H. pylori 139,761NoneVacuolating cytotoxin 393
Cluster 3 and 4
    AIDA-I Q03155 E. coli 132,272NoneAdherence 24
    IcsA AAA26547 S. flexneri 116,244NoneIntracellular motility 283
    MisL AAD16954 S. enterica 101,216NoneNone 35
    TibA AAD41751 E. coli 101,112NoneAdherence/invasion 292
    Ag43 P39180 E. coli 106,842RGD cell attachment sequence, aspartyl proteases active site, leucine zipper pattern, P-loop ATP/GTP-binding site motifBiofilm formation 204
    ShdA AAD25110 S. enterica 207,033Hemglutinin repeat, P-loop ATP/GTP-binding site motifAdherence, prolonged fecal shedding of bacteria 249
    AutA CAB89117 N. meningitidis 68,386RGD cell attachment sequenceNone 3
Cluster 5
    Tsh I54632 E. coli 148,227Peptidase S6 IgA endopeptidase, P-loop ATP/GTP-binding site motifHemaglobinase- and mucinase activity, hema binding 409
    SepA CAC05786 S. flexneri 145,938Peptidase S6 IgA endopeptidase, peptidase S1 chymotrypsinTissue invasion? 22
    EspC AAC44731 E. coli 140,861Peptidase S6 IgA endopeptidaseEnterotoxic activity 461
    EspP CAA66144 E. coli 141,758Peptidase S6 IgA endopeptidase, P-loop ATP/GTP-binding site motifCytotoxic activity 49
    Pet AAC26634 E. coli 139,769Peptidase S6 IgA endopeptidase, P-loop ATP/GTP-binding site motifEnterotoxic and cytopathic toxin effects, cleavage of spectrin 124
    Pic AAD23953 E. coli 146,450Peptidase S6 IgA endopeptidaseMucinase activity 196
    SigA AAF67320 S. flexneri 139,676Peptidase S6 IgA endopeptidaseCytopathic activity 5
    Sat AAG30168 E. coli 140,043Peptidase S6 IgA endopeptidase, P-loop ATP/GTP-binding site motifVacuolating cytotoxin 178
    Vat AAO21903 E. coli 148,291Peptidase S6 IgA endopeptidase, P-loop ATP/GTP-binding site motifVacuolating cytotoxin 384
    EpeA AAL18821 E. coli 147,340Peptidase S6 IgA endopeptidase, P-loop ATP/GTP-binding site motifMucinolytic activity 284
    EatA AAO17297 E. coli 147,696Peptidase S6 IgA endopeptidase, peptidase S1 chymotrypsin clan,None 385
    EspI CAC39286 E. coli 146,131Peptidase S6 IgA endopeptidase, P-loop ATP/GTP-binding site motifDegradation of plasma proteins 440
    EaaA AAF63237 E. coli 141,678Peptidase S6 IgA endopeptidaseNone 431
    EaaC AAF63038 E. coli 141,673Peptidase S6 IgA endopeptidaseNone 431
Cluster 6
    Pertactin P14283 B. pertussis 93,453RGD cell attachment sequenceAdherence 61
    BrkA AAA51646 B. pertussis 103,377RGD cell attachment sequenceSerum resistance 132
    Tef AAQ82668 B. pertussis 65,865RGD cell attachment sequenceTracheal colonization 141
    Vag8 AAC31247 B. pertussis 94,910RGD cell attachment sequenceNone 140
Cluster 7
    PmpD O84818 C. trachomatis 160,748NoneAdherence 464
    Pmp20 Q9Z812 C. pneumoniae 179,595Peptidase S1 chymotrypsinAdherence 234
    Pmp21 Q9Z6U5 C. pneumoniae 170,866NoneAdherence 234
Cluster 8
    IgA1 protease NP_283693 N. meningitidis 196,351Peptidase S6 IgA endopeptidaseDegradation of IgA1 LAMP-1, and synaptobrevin 300
    App CAC14670 N. meningitidis 159,072Peptidase S6 IgA endopeptidase, peptidase S1 chymotrypsin, RGD cell attachment sequenceAdherence 182
    IgA1 protease P45386 H. influenzae 202,957Peptidase S6 IgA endopeptidase, P-loop ATP/GTP-binding site motifDegradation of IgA1 406
    Hap P45387 H. influenzae 155,440Peptidase S6 IgA endopeptidase, peptidase S1 chymotrypsinAdherence, microcolony formation 466
Cluster 9
    rOmpA P15921 R. rickettsii 224,333NoneAdherence 10
    rOmpB Q53047 R. rickettsii 168,184NoneAdherence 163
Cluster 10
    ApeE AAC38796 S. enterica 69,862Lipolytic GDSL active-site enzymeHydrolysis of naphthyl esters 58
    EstA AAB61674 P. aeruginosa 69,609Lipolytic GDSL active-site enzymeLipolytic activity 533
    Lip-1 P40601 X. luminescens 70,717Lipolytic GDSL active-site enzyme, leucine zipper patternHydrolysis of naphthyl acetate, Tween 80, and p-nitrophenylpalmitate 523
    McaP AAP97134 M. catarrhalis 71,484Lipolytic GDSL active-site enzymeHydrolysis of phophatidylcholine and lysophosphatidylcholine, and adherence 489
Cluster 11
    BabA AAC38081 H. pylori 80,345NoneAdherence to Lewisb blood group antigen 215
    SabA AAD06240 H. pylori 72,169NoneAdherence to sialic Lewis antigen 308
    AlpA CAB05386 H. pylori 56,124Leucine zipper patternAdherence to Lewis x antigen 358
Unassigned
    Aae AAP21063 A. actinomycetemcomitans 100,609NoneAdherence 423
    NanB AAG35309 P. haemolytica 119,791RGD cell attachment sequence sialidaseSialidase activity 329