TABLE 2.

Bordetella autotransporter proteins

Known or predicted Bordetella auto- transporterORF designation and no. of residuesaAccession no.bReference(s) or best BLAST hit of passengerBvg reg- ulationcKnown (predicted) signal peptidedNotable motifse
BpBpaBb
Pertactin BP1054, 910 BPP1150, 922BB1366, 916 P14283 47, 61+34RGD (×2)
BrkA BP3494, 1,010 BPP0867, (173)BB0961, (127) AAA51646 132 +42RGD (×2)
SphB1f BP0216, 1,039 BPP0417, 1,043BB0419, 1,039 CAC44081 82, 83+NoSerine protease, lipoprotein
Tcf BP1201, 647 BB3291, (145) CAA08832 141 +(39)RGD
Vag8 BP2315, 915 BPP2415, 915BB1864, 915 AAC31247 140 +37RGD
Phg BP1767, 418 BPP1998, 415BB2246, 415 CAB38010 None0(35)None
SphB2 BP1660, 1,024 BPP2745, (193)BB2741, 1,025 12 0(37)None
SphB3 BP1110, 1,076 BPP2053, 1,076BB2301, 1,076 12 0(29)Thiol protease
BapA (AidB)g BP2224, 903 BPP2251, (295) [I]BB1649, 903 CAC14165 AIDA-10(30)hNone
BapBBP1200, (119) [I] BPP1815, 605 BB3292, 613 CAC14166 i NoneND(41)None
BapC (BatE)gBP2738, (102)BPP2591, (100) BB2033, 998 CAC14167 PertactinND(45)RGD (×2)
BatABP1793, (53) [I] BPP2022, 538 BB2270, 536NoneND(33)None
BatB BP0529, 2,300 BPP0452, 1,769BB0452, 2,300ShdAND(38)RGD (×2), P-loop, aspartyl protease
Novel BP0775, 340 BPP0337, 340BB0340, 340NoneND?None
Novel BP1344, 866 BPP2678, (717)BB2830, 866Ag43, AIDA-1, BapAND(36)RGD (×2)
NovelBP1610, (124) BPP2975, 937 BB2941, 937PertactinND32hNone
Novel BPP1617, 491 BB3111, 497NoneND(33)None
Novel BPP0449, 1,616 BB0450, 2,152ShdAND(40)hP-loop, lipase
Novel BPP0735, 996 BB0821, 999YapEND(30)RGD
Novel BPP0822, 1,196 BB0916, 1,196SSP-h1ND(23)hRGD
Novel BPP1618, 519 BB3110, 705TcfNDBP1618 (33), BB3110 (54)None
NovelBP2627, (178) BPP1256, 718 BB2324, 718(34)Lipoprotein
  • a ORF designation refers to genome sequence annotation. Bp, B. pertussis; Bpa, B. parapertussis; Bb, B. bronchiseptica. ORFs in bold type have been analyzed. When the number of amino acid residues is in parentheses, the ORF is truncated due to frameshift or insertion of a stop codon. Disruption by an insertion element is indicated by [I].

  • b GenBank accession number, if known.

  • c Regulation by the Bvg two-component system. +, activated genes; 0, not activated; ND, not determined.

  • d Values represent numbers of amino acids in the signal sequence; values in parentheses are derived from predictions based on SignalP (http://www.cbs.dtu.dk/services/SignalP-2.0/ ).

  • e Based on PROSITE patterns (http://ca.expasy.org/prosite/ ).

  • f Number of residues does not match the genome annotation.

  • g Alternate name for this putative autotransporter.

  • h Weakly predicted.

  • i Accession number annotation does not take into account the presence of the insertion element.