TABLE 1.

Overview of applications of IVET to isolate microbial genes upregulated in complex niches

Category and geneFunctionMicroorganismHost or environmentReference(s)
Auxotrophy-based selection
    purADe novo purine nucleotide biosynthesisSalmonella entericaMouse98, 156, 264
Pseudomonas aeruginosaMouse93
    purEKDe novo purine nucleotide biosynthesisPseudomonas aeruginosaBurned tissue92
Biofilm67
Mouse290
    thyADe novo thymidine nucleotide biosynthesisSalmonella entericaMouse156
    pyrBDe novo pyrimidine nucleotide biosynthesisPseudomonas putidaPhytophthora parasitica140
    ura5Uracil biosynthesisHistoplasma capsulatumMouse226
    asdDiaminopimelate biosynthesisPseudomonas aeruginosaMouse95
Pseudomonas putidaMaize63
Shigella flexneriMacrophage244
    dapBDiaminopimelate biosynthesisPseudomonas fluorescensSugar beet78, 302, 303
Pseudomonas fluorescensBulk soil245
Pseudomonas stutzeriRice224
    panBPantothenate biosynthesisPseudomonas fluorescensSugar beet218
    trpEGTryptophan biosynthesisRalstonia solanacearumTomato26
    galUGalactose metabolismKlebsiella pneumoniaeMouse133
    inhAMycolic acid biosynthesisMycobacterium tuberculosisMacrophage56
    ribBAHRiboflavin biosynthesisActinobacillus pleuropneumoniaePig76
Antibiotic resistance selection
    catChloramphenicol resistanceXanthomonas campestrisTurnip190
Burkholderia pseudomalleiMacrophage239
Shigella flexneriMacrophage14
Escherichia coliMouse126, 191
Helicobacter pyloriMouse8
Salmonella entericaMouse98, 157
Yersinia enterocoliticaMouse83, 300
Streptococcus gordoniiRabbit127, 191
Yersinia ruckeriFish65
    ermErythromycin resistanceLactobacillus reuteriMouse285
Streptococcus suisPig254
    tetTetracycline resistancePorphyromonas gingivalisMouse141, 298
    kanKanamycin resistancePasteurella multocidaMouse108
RIVET
    tnpRSite-specific recombinaseVibrio choleraeRabbit/mouse32, 137, 174, 191
Staphylococcus aureusMouse152
    creSite-specific recombinaseSalmonella entericaEpithelial cells5
Lactobacillus plantarumMouse24
    FLPSite-specific recombinaseCandida albicansMouse262
System-specific selection
    bacAMembrane protein necessary for bacteroid differentiationSinorhizobium melilotiAlfalfa188
    hlyListeriolysinListeria monocytogenesMouse55, 77
    metXWMethionine biosynthesisPseudomonas syringae pv. syringaeBean159
    hrcCComponent of TTSSPseudomonas syringae pv. tomatoArabidopsis thaliana16
    gfpGreen fluorescent proteinErwinia chrysanthemiSpinach299