TABLE 2.

Substrate specificity of CDP-archaeol synthase from Methanothermobacter thermautotrophicusa

SubstrateHydrocarbonNo. of double bonds/side chainLinkageGP backboneCDP-archaeol synthase activity (%)
2,3-GG-GP etherGeranylgeranyl4EtherG-1-P100
1,2-GG-GP etherGeranylgeranyl4EtherG-3-P94
2,3-Phyta-GP etherPhytanyl0EtherG-1-P3
2,3-Phyto-GP etherPhytyl1EtherG-1-P0
2,3-Ole-GP etherOleyl1EtherG-1-P0
2,3-GG-GP esterGeranylgeranoyl4EsterG-1-P122
1,2-GG-GP esterGeranylgeranoyl4EsterG-3-P50
rac-GG-GP esterGeranylgeranoyl4Ester rac -GP81
2,3-Ole-GP esterOleoyl1EsterG-1-P13
rac-Ole-GP esterOleoyl1Ester rac -GP3
  • a See reference 70. Each substrate (2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate) analog synthesized was incubated with [5-3H]CTP and the membrane fraction of Methanothermobacter thermautotrophicus at 55°C for 2 h. After the reaction, chloroform-extractable 3H was counted.