TABLE 3.

Substrate specificity of archaetidylserine synthase from Methanothermobacter thermautotrophicusa

SubstratebHydrocarbonNo. of double bonds/side chainLinkageGP backboneRelative CDP-archaeol synthase activity (%)
CDP-2,3-GG-Gro etherGeranylgeranyl4EtherG-1-P100
CDP-1,2-GG-Gro etherGeranylgeranyl4EtherG-3-P149
CDP-2,3-Phyta-Gro etherPhytanyl0EtherG-1-P86
CDP-1,2-Ole-Gro etherOleyl1EtherG-3-P64
CDP-2,3-Ole-Gro esterOleoyl1EsterG-1-P280
CDP-1,2-acyl-Gro esterFatty acylEsterG-3-P199
  • a See reference 69. Each substrate (CDP-2,3-di-O-geranylgeranyl-sn-glycerol or CDP-archaeol) analog synthesized was incubated with [3-3H]l-serine and cell-free homogenate of Methanothermobacter thermautotrophicus at 60°C for 10 min. After the reaction, chloroform-extractable 3H was counted.

  • b Abbreviations: GG, geranylgeranyl; Gro, glycerol; Phyta, phytanyl; Ole, ole(o)yl; acyl, mixed fatty acyl.