TABLE 6.

Degenerate PCR primers based on conserved motifs in retroviruses

Protein(s)Motif(s)aGenusReference(s)
RTLPQG, YXDDAll106, 124, 189, 214, 251, 301, 421, 440, 447
RTSeveralLentivirus122
RT (nested primers)SeveralAll284
RT (heminested primers)LPQG, YXDDAll246, 385, 493
PRDTGBetaretrovirus189
PR, RTGRD, LPQGBetaretrovirus324
PR, RTDTG, YXDDAll207, 380, 491
PR, RTDTGA, VLPQG, YMDDAll381
IN (nested primers)SeveralSpumaretrovirus436
NC, INSeveralBetaretrovirus190
PolSeveralDeltaretrovirus128, 394
Pol (nested primers)SeveralLentivirus169
Gag, PolSeveralSpumavirus (of primates)47, 73
Gag, PolSeveralNon-HERV80
Gag, PolSeveralBetaretrovirus (of primates)282
  • a Consensus peptide motifs used.