TABLE 6.

Degenerate PCR primers based on conserved motifs in retroviruses

Protein(s)Motif(s)aGenusReference(s)
RTLPQG, YXDDAll 106, 124, 189, 214, 251, 301, 421, 440, 447
RTSeveral Lentivirus 122
RT (nested primers)SeveralAll 284
RT (heminested primers)LPQG, YXDDAll 246, 385, 493
PRDTG Betaretrovirus 189
PR, RTGRD, LPQG Betaretrovirus 324
PR, RTDTG, YXDDAll 207, 380, 491
PR, RTDTGA, VLPQG, YMDDAll 381
IN (nested primers)Several Spumaretrovirus 436
NC, INSeveral Betaretrovirus 190
PolSeveral Deltaretrovirus 128, 394
Pol (nested primers)Several Lentivirus 169
Gag, PolSeveral Spumavirus (of primates) 47, 73
Gag, PolSeveralNon-HERV 80
Gag, PolSeveral Betaretrovirus (of primates) 282
  • a Consensus peptide motifs used.