TABLE 3.

Production of MSH and MSH intermediates by M. smegmatis mutants

EnzymeStrainConcn (μmol/g dry weight) of MSH or intermediate
GlcNAc-InsGlcN-InsCys-GlcN-InsMSH
Wild typeamc2155<0.10.2-1.0∼0.00810 ± 3
MshAb mshA::Tn5<0.01<0.01<0.01<0.01
MshBcMyco5042.6 ± 0.2<0.01<0.021.0 ± 0.2
MshCdTn1NDe2.6<0.002<0.004
MshDf mshD::Tn5ND0.35 ± 0.050.6-20.12 ± 0.01g
  • a From references 1, 59, and 94.

  • b From reference 91.

  • c From reference 117 and G. L. Newton, unpublished data.

  • d From reference 118.

  • e ND, not determined.

  • f From references 59 and 94.

  • g Two to 3 μmol/g of formyl-Cys-GlcN-Ins and 0.12 ± 0.01 μmol/g of succinyl-Cys-GlcN-Ins were also produced.