TABLE 4.

F. tularensis subsp. tularensis genes involved in pathogenesis

Locus tagNameFunctionMethod(s)aCells/animals in which mutant strain is attenuatedbReference(s)
FTT0026c fslE Siderophore uptakeTp, ArBALB/c 100, 159
FTT0056cFTT0056cMajor facilitator superfamily transport proteinTpHep G2 155
FTT0069cFTT0069UnannotatedTpBALB/c 100
FTT0107c dsbB Transposon mutantTp, ArC57BL/6 157
FTT0107c dsbB Disulfide bond formation proteinTpHep G2, J774A.1 155
FTT0118 prfC Peptide chain release factor 3TpBALB/c 100
FTT0129FTT0129Major facilitator superfamily transport proteinTpHep G2 155
FTT0132 glpA Glycerol-3-phosphate dehydrogenaseTpBALB/c 100
FTT0141 rplA 50S ribosomal protein L1TpBALB/c 100
FTT0156FTT0156Acid phosphataseTpBALB/c 100
FTT0203c purH Bifunctional purine biosynthesis proteinTpHep G2 155
FTT0204 purA Adenylosuccinate synthetaseTpHep G2 155
FTT0245 usp Universal stress proteinTpBALB/c 100
FTT0250 ppdK Phosphoenolpyruvate synthase/pyruvate phosphate dikinaseTpBALB/c 100
FTT0290 moxR MoxR-like ATPaseTpBALB/c 100
FTT0425 asd Aspartate semialdehyde dehydrogenaseTpBALB/c 100
FTT0435 ctv Carbon-nitrogen hydrolaseArBMDM, MH-S, BALB/c 123
FTT0444 tet Drug:H+ antiporter-1 (DNA1) family proteinTpBALB/c 100
FTT0486 mutL DNA mismatch repair enzyme with ATPase activityTpBALB/c 100
FTT0588 aroA 3-Phosphoshikimate I-carboxyvinyl transferaseTpHep G2 155
FTT0609FTT0609UnannotatedTpBALB/c 100
FTT0623 tig Trigger factor protein (peptidyl-prolyl cis/trans isomerase), chaperoneTpBALB/c 100
FTT0626 lon DNA binding, ATP-dependent protease LATpBALB/c 100
FTT0654 elbB DJ-1/PfpI family proteinTpBALB/c 100
FTT0687c hslU ATP-dependent protease HslVU, ATPase subunitTpBALB/c 100
FTT0862c htpX Zn-dependent protease with chaperone functionTpBALB/c 100
FTT0876c aroC Chorismate synthaseTpBALB/c 100
FTT0891FTT0891Conserved hypothetical membrane proteinTpBALB/c 100
FTT0893-FTT0894 purMCD Purine biosynthesisArMDM, J774A.1, A549, BALB/c 146
FTT0918FTT0918Hypothetical proteinArBALB/c 204
FTT1087c rep UvrD/REP superfamily I DNA and RNA helicasesTpBALB/c 100
FTT1103FTT1103Conserved hypothetical lipoproteinTp/ArHep G2, BALB/c, C57BL/6 155, 157
FTT1120c tgt Queuine tRNA-ribosyltransferaseTpBALB/c 100
FTT1130c cphA Cyanophycin synthetaseTpBALB/c 100
FTT1158c pilO Type IV pilus glycosylation proteinTpBALB/c 100
FTT1179 bipA GTP binding translational elongation, factor Tu and G family proteinTpBALB/c 100
FTT11802c trpE Anthranilate synthase component ITpBALB/c 100
FTT1181c ggt Gamma-glutamyltranspeptidaseAr, TpBALB/c, HepG2 100, 155
FTT1234FTT1234Choloylglycine hydrolase family proteinTpHep G2 155
FTT1236FTT1236Hypothetical proteinTpHep G2, J774A.1 155
FTT1244c yfiO Conserved hypothetical lipoproteinTpHep G2 155
FTT1278c rnhB RNase HIITpBALB/c 100
FTT1310c hflB ATP-dependent metalloproteaseTpHep G2 155
FTT1312c uvrA DNA excision repair enzyme, subunit ATpBALB/c 100
FTT1356, FTT1711 idlD Intracellular growth locus DArBALB/c 100
FTT1357c, FTT1712c iglC2, iglC1Intracellular growth locus CArBALB/c 204
FTT1358, FTT1713 iglB Intracellular growth locus BArBALB/c 100
FTT1455 wbtI Sugar transamine/perosamine synthetaseArBALB/c 100
FTT1455 wbtI LPS O-antigen synthesisTpBALB/c 100
FTT1459c-FTN1461c wbtDEF LPS O-antigen biosynthetic clusterAr/InJ774A.1, BALB/c 200
FTT1490FTT1490Na+/H+ antiporterTpHep G2 155
FTT1542c omp26 Protein of unknown functionTpBALB/c 100
FTT1561 kdtA 3-Deoxy-d-manno-octulosonic-acid transferaseArBALB/c 100
FTT1631 glpX Fructose 1,6-bisphosphatase IIArBALB/c 100
FTT1640cFTT1640Activator of osmoprotectant transporter ProP, fragmentTpBALB/c 100
FTT1663 carA Carbamoylphosphate synthase small chainTpHep G2 155
FTT1664 carB Carbamoylphosphate synthase large chainTpHep G2 155
FTT1665 pyrB Aspartate carbamoyltransferaseTpHep G2 155
FTT1720c purL Phosphoribosylformylglycinamide synthaseTpBALB/c, Hep G2 100, 155
FTT1721 purF AmidophosphoribosyltransferaseTpBALB/c, Hep G2 100, 155
FTT1724c tolC Outer membrane efflux proteinTpBALB/c 100
FTT1744c ipdC Indolepyruvate decarboxylaseTpBALB/c 100
FTT1762cFTT1762cAcetyltransferase proteinTpHep G2 155
FTT1773c trpB Tryptophan synthase beta chainTpBALB/c 100
FTT1782c yjjK ABC transporter ATP binding proteinTpBALB/c 100
  • a Ar, allelic replacement; In, insertion; Tp, transposon insertion.

  • b BMDM, bone marrow-derived macrophages; MDM, moncyte-derived macrophages; HepG2, human hepatocellularcarcinoma cells; MH-S, murine alveolar macrophages.