TABLE 4.

F. tularensis subsp. tularensis genes involved in pathogenesis

Locus tagNameFunctionMethod(s)aCells/animals in which mutant strain is attenuatedbReference(s)
FTT0026cfslESiderophore uptakeTp, ArBALB/c100, 159
FTT0056cFTT0056cMajor facilitator superfamily transport proteinTpHep G2155
FTT0069cFTT0069UnannotatedTpBALB/c100
FTT0107cdsbBTransposon mutantTp, ArC57BL/6157
FTT0107cdsbBDisulfide bond formation proteinTpHep G2, J774A.1155
FTT0118prfCPeptide chain release factor 3TpBALB/c100
FTT0129FTT0129Major facilitator superfamily transport proteinTpHep G2155
FTT0132glpAGlycerol-3-phosphate dehydrogenaseTpBALB/c100
FTT0141rplA50S ribosomal protein L1TpBALB/c100
FTT0156FTT0156Acid phosphataseTpBALB/c100
FTT0203cpurHBifunctional purine biosynthesis proteinTpHep G2155
FTT0204purAAdenylosuccinate synthetaseTpHep G2155
FTT0245uspUniversal stress proteinTpBALB/c100
FTT0250ppdKPhosphoenolpyruvate synthase/pyruvate phosphate dikinaseTpBALB/c100
FTT0290moxRMoxR-like ATPaseTpBALB/c100
FTT0425asdAspartate semialdehyde dehydrogenaseTpBALB/c100
FTT0435ctvCarbon-nitrogen hydrolaseArBMDM, MH-S, BALB/c123
FTT0444tetDrug:H+ antiporter-1 (DNA1) family proteinTpBALB/c100
FTT0486mutLDNA mismatch repair enzyme with ATPase activityTpBALB/c100
FTT0588aroA3-Phosphoshikimate I-carboxyvinyl transferaseTpHep G2155
FTT0609FTT0609UnannotatedTpBALB/c100
FTT0623tigTrigger factor protein (peptidyl-prolyl cis/trans isomerase), chaperoneTpBALB/c100
FTT0626lonDNA binding, ATP-dependent protease LATpBALB/c100
FTT0654elbBDJ-1/PfpI family proteinTpBALB/c100
FTT0687chslUATP-dependent protease HslVU, ATPase subunitTpBALB/c100
FTT0862chtpXZn-dependent protease with chaperone functionTpBALB/c100
FTT0876caroCChorismate synthaseTpBALB/c100
FTT0891FTT0891Conserved hypothetical membrane proteinTpBALB/c100
FTT0893-FTT0894purMCDPurine biosynthesisArMDM, J774A.1, A549, BALB/c146
FTT0918FTT0918Hypothetical proteinArBALB/c204
FTT1087crepUvrD/REP superfamily I DNA and RNA helicasesTpBALB/c100
FTT1103FTT1103Conserved hypothetical lipoproteinTp/ArHep G2, BALB/c, C57BL/6155, 157
FTT1120ctgtQueuine tRNA-ribosyltransferaseTpBALB/c100
FTT1130ccphACyanophycin synthetaseTpBALB/c100
FTT1158cpilOType IV pilus glycosylation proteinTpBALB/c100
FTT1179bipAGTP binding translational elongation, factor Tu and G family proteinTpBALB/c100
FTT11802ctrpEAnthranilate synthase component ITpBALB/c100
FTT1181cggtGamma-glutamyltranspeptidaseAr, TpBALB/c, HepG2100, 155
FTT1234FTT1234Choloylglycine hydrolase family proteinTpHep G2155
FTT1236FTT1236Hypothetical proteinTpHep G2, J774A.1155
FTT1244cyfiOConserved hypothetical lipoproteinTpHep G2155
FTT1278crnhBRNase HIITpBALB/c100
FTT1310chflBATP-dependent metalloproteaseTpHep G2155
FTT1312cuvrADNA excision repair enzyme, subunit ATpBALB/c100
FTT1356, FTT1711idlDIntracellular growth locus DArBALB/c100
FTT1357c, FTT1712ciglC2, iglC1Intracellular growth locus CArBALB/c204
FTT1358, FTT1713iglBIntracellular growth locus BArBALB/c100
FTT1455wbtISugar transamine/perosamine synthetaseArBALB/c100
FTT1455wbtILPS O-antigen synthesisTpBALB/c100
FTT1459c-FTN1461cwbtDEFLPS O-antigen biosynthetic clusterAr/InJ774A.1, BALB/c200
FTT1490FTT1490Na+/H+ antiporterTpHep G2155
FTT1542comp26Protein of unknown functionTpBALB/c100
FTT1561kdtA3-Deoxy-d-manno-octulosonic-acid transferaseArBALB/c100
FTT1631glpXFructose 1,6-bisphosphatase IIArBALB/c100
FTT1640cFTT1640Activator of osmoprotectant transporter ProP, fragmentTpBALB/c100
FTT1663carACarbamoylphosphate synthase small chainTpHep G2155
FTT1664carBCarbamoylphosphate synthase large chainTpHep G2155
FTT1665pyrBAspartate carbamoyltransferaseTpHep G2155
FTT1720cpurLPhosphoribosylformylglycinamide synthaseTpBALB/c, Hep G2100, 155
FTT1721purFAmidophosphoribosyltransferaseTpBALB/c, Hep G2100, 155
FTT1724ctolCOuter membrane efflux proteinTpBALB/c100
FTT1744cipdCIndolepyruvate decarboxylaseTpBALB/c100
FTT1762cFTT1762cAcetyltransferase proteinTpHep G2155
FTT1773ctrpBTryptophan synthase beta chainTpBALB/c100
FTT1782cyjjKABC transporter ATP binding proteinTpBALB/c100
  • a Ar, allelic replacement; In, insertion; Tp, transposon insertion.

  • b BMDM, bone marrow-derived macrophages; MDM, moncyte-derived macrophages; HepG2, human hepatocellularcarcinoma cells; MH-S, murine alveolar macrophages.