TABLE 6.

Francisella live vaccine candidates

Species or subspeciesLocus tag for attenuating mutationAnnotationVaccination routea (dose, CFU)Challenge strainBoostChallenge route (dose, CFU)Host mouse strain(s)ProtectionReference
F. novicida None (wild type)i.d. (100)FSC033NoAerosol (10)BALB/cNo 182
FTN_1645 atpC i.n. (149)U112Noi.n. (25)C57BL/6Yes 106
FTN_0178 purA i.p. (3.3 × 106)U112Noi.p. (170)BALB/cNo 158
FTN_1700 purF i.p. (6.95 × 103)U112Noi.p. (170)BALB/cYes 158
Schu S4Noi.p. (100)BALB/cNo 158
FTN_1465 pmrA i.n. (106)Schu S4Noi.n. (100)BALB/cNo 134
U112Noi.n. (106)BALB/cYes 134
FTN_1608 dsbB i.n. (6 × 105)U112Noi.n. (6 × 107)BALB/cYes 199
FTN_0714FTT0742i.n. (6 × 107)U112Noi.n. (6 × 107)BALB/cYes 199
FTN_1310 pdpB i.n. (6 × 107)U112Noi.n. (6 × 107)BALB/cYes 199
FTN_0337 fumA i.n. (6 × 105)U112Noi.n. (6 × 107)BALB/cYes 199
FTN_0020 carB i.n. (6 × 103)U112Noi.n. (8 × 105)BALB/cYes 199
FTN_1322 iglC i.n. (106)U112Noi.n. (103)BALB/cYes 142
i.n. (104)BALB/cYes 142
i.n. (105)BALB/cNo 142
FTN_1322 iglB i.n. (106)U112Noi.n. (103)BALB/cYes 39
LVSNoi.n. (3 × 104)BALB/cYes 39
Oral (103)Schu S4Yesi.n. (52)BALB/cNo 39
FTN_1290 mglA Aerosol (105)U112NoAerosol (35-8 × 104)BALB/c, C57BL/6No 214
FTN_0050, FTN_1556, FTN_1061, FTN_0954 acpABCH i.n. (103)U112Noi.n. (106)BALB/cYes 135
i.n. (106)U112Noi.n. (106)BALB/cYes 135
FTN_0090, FTN_1061, FTN_0954 acpCH or acpACHi.n. (103)U112Noi.n. (103)BALB/cNo 135
FTN_0546 flmK s.c. (400-500)U112Nos.c. (660)BALB/c, C57BL/6Yes 101
Aerosol (100)U112Noi.n. (50)BALB/cYes 101
F. tularensis subsp. holarcticaNone (wild type)i.n. (200)Schu S4Noi.n. (100)BALB/cYes 146
i.n. (2,000)BALB/cNo 146
Yesi.d. (100, 2,000)BALB/cYes 146
FTL_0094 clpB i.p. (13)LVSNoi.p. (1 × 104)BALB/cYes 129
i.p. (1,300)LVSNoi.p. (1 × 104)BALB/cYes 129
FTL0395-FTL0396 purMCD i.n. (106)Schu S4Noi.n. (100, 2,000)BALB/cNo 146
i.d. (100, 2,000)BALB/cNo 146
Yesi.n. (100)BALB/cYes 146
i.n. (2,000)BALB/cNo 146
i.d. (100, 2,000)BALB/cYes 146
FTL0552 pmrA i.n. (105)Schu S4Noi.n. (102)C57BL/6, BALB/cYes 165
FTL0421 lpnA i.d. (105)LVSNoi.d. (107)C3H/HeNYes 62
FTL_1071 guaA (2.2 × 107)LVSNo2.8 × 105BALB/cYes 170
FTL_1478 guaB (3.6 × 107)LVSNo2.8 × 105BALB/c 170
FTL_1793 sodB i.n. (5,000)Schu S4Noi.n. (14 × 103)C57BL/6Yes 9
i.n. (500)LVSYesi.n. (1.3 × 106)C57BL/6Yes 9
Schu S4Yesi.n. (104)C57BL/6No 9
FTL_0592 wbtA i.d. (1.5 × 108)FSC 108Yesi.d. (17)BALB/cYes 180
Schu S4Yesi.d. (10)BALB/cYes 180
F. tularensis subsp. tularensisFTT0107c dsbB i.n. (6.8 × 103)Schu S4Noi.n. (13-13,000)C57BL/6No 156
FTT0893-FTT0894 purMCD i.n. (104)Schu S4Noi.n. (100, 2,000)BALB/cNo 146
i.d. (100, 2,000)BALB/cYes 146
Yesi.n. (100)BALB/cYes 146
i.n. (2,000)BALB/cNo 146
i.d. (100, 2,000)BALB/cYes 146
FTT0918FTT0918i.d. (105)FSC033Noi.d. (500)BALB/cYes 204
Aerosol (10)BALB/cYes 204
FTT1357c, FTT1712c iglC, iglC1i.d. (106-107)FSC033Noi.d. (500), aerosol (10)BALB/cNo 204
FTT1459c-FTT1461c wbtDEF s.c. (105)Schu S4Nos.c. (100)BALB/cNo 200
FTT1103FTT1103i.n. (1.6 × 108)Schu S4Noi.n. (1,000)C57BL/6, BALB/cYes 157
  • a i.d., intradermal; i.n., intranasal; i.p., intraperitoneal; s.c., subcutaneous.