TABLE 12.

Genes involved in peptidoglycan and rhamnose biosynthesis in bifidobacteria

Functional category and EC no.KEGG annotationLocus tag
B. longum subsp. longumB. longum subsp. infantis ATCC 15697B. adolescentis ATCC 15703B. dentium Bd1B. animalis subsp. lactis
DJO10ANCC2705AD011Bl-04DSM 10140Bb-12
Peptidoglycan biosynthesis
    2.5.1.7murA; UDP-N-acetylglucosamine 1-carboxyvinyltransferaseBLD_1600BL1267Blon_2321BAD_0183BDP_0275BLA_0233Balac_0245Balat_0245Unannotatedc
    1.1.1.158murB; UDP-N-acetylmuramate dehydrogenaseBLD_1681BL1561Blon_2254BAD_0294BDP_0408BLA_0345Balac_0364Balat_0364BIF_00678
    6.3.2.8murC; UDP-N-acetylmuramate-alanine ligaseBLD_0182BL1324Blon_0857BAD_1100BDP_1541BLA_0775Balac_1197Balat_1197BIF_00646
    6.3.2.9murD; UDP-N-acetylmuramoylalanine-d-glutamate ligaseBLD_0179BL1321Blon_0854BAD_1103BDP_1544BLA_0778Balac_1200Balat_1200BIF_00509
    6.3.2.13murE; UDP-N-acetylmuramoylalanyl-d-glutamate-2,6-diaminopimelate ligaseBLD_0221BL1356Blon_0893BAD_1085BDP_1521BLA_0746Balac_1168Balat_1168BIF_01077
    6.3.2.4ddlA; d-alanine-d-alanine ligaseBLD_1112BL0345Blon_0320BAD_0186BDP_0283BLA_0236Balac_0248Balat_0248BIF_01368
    6.3.2.10murF; UDP-N-acetylmuramoylalanyl-d-glutamyl-2,6-diaminopimelate-d-alanyl-d-alanine ligaseBLD_0177BL1319Blon_0852BAD_1105BDP_1546BLA_0780Balac_1202Balat_1202BIF_00378
    2.7.8.13mraY; phospho-N-acetylmuramoyl-pentapeptide transferaseBLD_0178BL1320Blon_0853BAD_1104BDP_1545BLA_0779Balac_1201Balat_1201BIF_00379
    2.4.1.227murG; UDP-N-acetylglucosamine-N-acetylmuramoyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferaseBLD_0181BL1323Blon_0856BAD_1101BDP_1542BLA_0776Balac_1198Balat_1198Unannotatedd
    2.4.1.129Peptidoglycan glycosyltransferaseBLD_0175BL1317Blon_0079, Blon_0850BAD_0040, BAD_1107BDP_1548BLA_0077, BLA_0782Balac_1204Balat_1204BIF_00315
    3.6.1.27bacA; undecaprenyl-diphosphataseBLD_0446BL0721Blon_1151BAD_0825BDP_1149BLA_1353Balac_0838Balat_0838BIF_01744
    3.4.16.4dacB; d-alanyl-d-alanine carboxypeptidaseBLD_1845BL1679Blon_0546BAD_0407BDP_0546BLA_0471Balac_0496Balat_0496BIF_01386
Rhamnose biosynthesis
    2.7.7.9ugpA; UTP-glucose-1-phosphate uridylyltransferaseBLD_0464BL0739Blon_1169BAD_0816BDP_1163BLA_1330Balac_0808Balat_0808BIF_00196
    2.7.7.24rfbA; glucose-1-phosphate thymidylyltransferaseBLD_1570BL0227BAD_1509BDP_1860BLA_0581Balac_1376Balat_1376BIF_01544
    4.2.1.46rfbB; dTDP-glucose 4,6-dehydrataseBLD_1568BL0229Blon_2390BAD_1507BDP_1862BLA_0583Balac_1378Balat_1378BIF_01541
    5.1.3.13rfbC (fuseda); dTDP-4-dehydrorhamnose 3,5-epimeraseBLD_1569BL0228BAD_1508UnannotatedbBLA_0582Balac_1377Balat_1377BIF_01543
    1.1.1.133rfbD (fuseda); dTDP-4-dehydrorhamnose reductase
  • a rfbC and rfbD are fused in bifidobacteria.

  • b This gene (nucleotides [nt] 2,123,091 to 2,124,545) in B. dentium Bd1 was not annotated in the original annotation data (CP001750).

  • c This gene (nt 928,929 to 930,257) in B. animalis subsp. lactis Bb-12 was not annotated in the original annotation data (CP001853).

  • d This gene (nt 77,792-78,976, complement) in B. animalis subsp. lactis Bb-12 was not annotated in the original annotation data (CP001853).