TABLE 1.

eSTKs in bacteriaa

SpecieseSTKSubstrateFunctionPhospho-residue(s)MethodologyReference(s)
Bacillus subtilisPrkCAlsDα-Acetolactate decarboxylase; central metabolismS88In vitro kinase assay, mass spectrometry1a, 141
CpgAGTPase; peptidoglycan depositionThr(s)/Ser(s)In vitro kinase assay, site-directed mutagenesis1
EF-GElongation factor; protein translationThr(s)In vivo immunoprecipitation, in vitro kinase assay50, 159, 160
EF-TuElongation factor; protein translationT384In vitro kinase assay, site-directed mutagenesis1
GlnAGlutamine synthetase; central metabolismS207, T26, T147, T286In vitro kinase assay, mass spectrometry141
IcdCentral metabolismT138, T14, T396In vitro kinase assay, mass spectrometry141
HPrKinase, phosphotransferase systemS12In vitro kinase assay, mass spectrometry141
YezBStressThr(s)In vitro kinase assay, phospho-amino acid analysis, 2D electrophoresis1
YwjHTransaldolase; central metabolismS55, T26, T82, T125, T159, T184In vitro kinase assay, mass spectrometry141
Chlamydia trachomatisPkn1IncGPathogenesisNABacterial two-hybrid assay, in vitro kinase assay186
PknDPkn1eSTKNABacterial two-hybrid assay, immunoprecipitation, in vitro kinase assay186
Corynebacterium glutamicumPknAFtsZCell divisionT108In vitro kinase assay, 2D electrophoresis, mass spectrometry157
MurCCell wall synthesisT51, T120, T133, T16, T362, T365In vitro kinase assay, mass spectrometry, site-directed mutagenesis48
PknGSoluble eSTKT451, T787In vitro kinase assay49
OdhIGlutamate catabolismT15In vitro kinase assay, mass spectrometry13, 49, 157
PknBFtsZCell divisionNAIn vitro kinase assay157
OdhIGlutamate catabolismT15In vivo and in vitro kinase assays13, 49, 157
PknGOdhIGlutamate catabolismT14In vivo 2D electrophoresis, in vitro kinase assay, mass spectrometry, site-directed mutagenesis49, 121, 157
PknLFtsZCell divisionT63, S353, T388In vitro kinase assay, 2D electrophoresis, mass spectrometry157
Mycobacterium tuberculosisPknAEmbRArabinan synthesis, cell wallNAIn vitro kinase assay162
FadDMycolic acid biosynthesisThr(s)In vitro kinase assay, phospho-amino acid analysis109
FabHMycolic acid pathway; cell wall biosynthesisT45In vitro kinase assay, mass spectrometry, site-directed mutagenesis187
FipACell division under oxidative stressT77In vivo and in vitro kinase assays, mass spectrometry175
FtsZCell divisionT343In vitro kinase assay, mass spectrometry175, 179
GlmUCell wall synthesisThr(s)In vitro kinase assay135
GroEL1Heat shock proteinNAIn vitro kinase assays, mass spectrometry23
KasAMycolic acid biosynthesisThr(s)In vitro kinase assay, phospho-amino acid analysis109
KasBMycolic acid biosynthesisThr(s)In vitro kinase assay, phospho-amino acid analysis109
MabAMycolic acid biosynthesisT21, T114, T191In vitro kinase assay, mass spectrometry, site-directed mutagenesis188
MurDCell divisionNAIn vitro kinase assay178
PknBeSTKNAIn vitro kinase assay75
Rv1422NAT325In vivo and in vitro kinase assays, mass spectrometry, site-directed mutagenesis75
Wag31Cell divisionT73In vivo and in vitro kinase assays, mass spectrometry, site-directed mutagenesis75
PknBEmbRArabinan synthesis, cell wallNAIn vitro kinase assay162
FadDMycolic acid biosynthesisNAIn vitro kinase assay109
GarAGlycogen recycling, tricarboxylic acid cycleT22Phosphoproteomic kinase assays, site-directed mutagenesis189
GlmUCell wall synthesisThr(s)In vitro kinase assay135
GroEL1Heat shock proteinNAIn vitro kinase assays, mass spectrometry23
KasAMycolic acid biosynthesisNAIn vitro kinase assay109
KasBMycolic acid biosynthesisNAIn vitro kinase assay109
MabAMycolic acid biosynthesisT21, T114, T191In vitro kinase assay, mass spectrometry, site-directed mutagenesis188
PBPACell wall synthesisT437, T362In vitro kinase assay, mass spectrometry, site-directed mutagenesis32
PknAeSTKNAIn vitro kinase assay75
RshASigH anti-sigma factor, oxidative stressT94In vivo and in vitro kinase assays, mass spectrometry, site-directed mutagenesis136
Rv0020cFHA-containing proteinNAIn vitro kinase assay60
Rv1422NAT325In vitro kinase assay, mass spectrometry, site-directed mutagenesis75
Rv1747Putative ABC transporterNAIn vitro kinase assay60
SigHAlternate sigma factor; oxidative stressT26, T106In vivo and in vitro kinase assays, mass spectrometry, site-directed mutagenesis136
PknDFadDMycolic acid biosynthesisNAIn vitro kinase assay109
FabHMycolic acid pathway, cell wall biosynthesisT45In vitro kinase assay, mass spectrometry, site-directed mutagenesis187
GarAGlycogen recycling, tricarboxylic acid cycleNAIn vitro kinase assays189
GroEL1Heat shock proteinNAIn vitro kinase assays, mass spectrometry23
KasAMycolic acid biosynthesisNAIn vitro kinase assay109
KasBMycolic acid biosynthesisNAIn vitro kinase assay109
MabAMycolic acid biosynthesisT21, T114, T191In vitro kinase assay, mass spectrometry, site-directed mutagenesis188
Mmpl7Membrane transporter; resistance, nodulation, and cell division familyNA2D electrophoresis, mass spectrometry139
Rv0516cAnti-anti-sigma factorT2In vivo and in vitro kinase assays, mass spectrometry, site-directed mutagenesis59a
Rv1747Putative ABC transporterNAIn vitro kinase assay60
PknEFadDMycolic acid biosynthesisNAIn vitro kinase assay109
FabHMycolic acid pathway, cell wall biosynthesisT45In vitro kinase assay, mass spectrometry, site-directed mutagenesis187
GarAGlycogen recycling, tricarboxylic acid cycleNAIn vitro kinase assays189
GroEL1Heat shock proteinNAIn vitro kinase assays, mass spectrometry23
KasAMycolic acid biosynthesisNAIn vitro kinase assay109
KAsBMycolic acid biosynthesisNAIn vitro kinase assay109
MabAMycolic acid biosynthesisT21, T114, T191In vitro kinase assay, mass spectrometry, site-directed mutagenesis188
Rv1747Putative ABC transporterNAIn vitro kinase assay60
PknFFadDMycolic acid biosynthesisNAIn vitro kinase assay112
FabHMycolic acid synthesis, cell wallT45In vivo and in vitro kinase assays, mass spectrometry, site-directed mutagenesis187
GarAGlycogen recycling, tricarboxylic acid cycleNAIn vitro kinase assays189
GroEL1Heat shock proteinT25, T54In vivo and in vitro kinase assays, mass spectrometry, site-directed mutagenesis23
KasAMycolic acid biosynthesisNAIn vitro kinase assay109
KasBMycolic acid biosynthesisNAIn vitro kinase assay109
Rv0020cFHA-containing proteinNAIn vitro kinase assay60
Rv1747Putative ABC transporterNAIn vitro kinase assay112
PknGGarAGlycogen recycling, tricarboxylic acid cycleT21Kinase assays, mass spectrometry, site-directed mutagenesis, in vivo studies128
PknHDacB1Penicillin-binding protein, cell wallThr(s)In vitro kinase assay, site-directed mutagenesis201
DosSDormancyT198, T205In vitro kinase assays, mass spectrometry, site-directed mutagenesis25
EmbRArabinan synthesis, cell wallThr(s)In vitro kinase assay111
FadDMycolic acid biosynthesisNAIn vitro kinase assay109
FabHMycolic acid pathway, cell wall biosynthesisT45In vitro kinase assay, mass spectrometry, site-directed mutagenesis187
GroEL1Heat shock proteinNAIn vitro kinase assays, mass spectrometry23
KasAMycolic acid biosynthesisNAIn vitro kinase assay109
KasBMycolic acid biosynthesisNAIn vitro kinase assay109
Rv0681TetR class transcription factorT170In vitro kinase assay, site-directed mutagenesis201
PknIFadDMycolic acid biosynthesisNAIn vitro kinase assay109
PknJPyruvate kinase AGlycolysisS37In vitro kinase assay6
EmbRArabinan synthesis, cell wallNAIn vitro kinase assay71
MmaA4Mycolic acid biosynthesisNAIn vitro kinase assay71
PepEDipeptidaseNAIn vitro kinase assay71
PknKFadDMycolic acid biosynthesisNAIn vitro kinase assay109
VirSTranscriptional regulatorThr(s)In vitro kinase assay, phospho-amino acid analysis84
PknLFadDMycolic acid biosynthesisNAIn vitro kinase assay109
GroEL1Heat shock proteinNAIn vitro kinase assays, mass spectrometry23
KasAMycolic acid biosynthesisNAIn vitro kinase assay109
KasBMycolic acid biosynthesisNAIn vitro kinase assay109
MabAMycolic acid biosynthesisT21, T114, T191In vitro kinase assay, mass spectrometry, site-directed mutagenesis188
Rv2175cDNA-binding protein, putative cell wall/cell division proteinT9In vivo and in vitro kinase assays, mass spectrometry, site-directed mutagenesis, phospho-amino acid analysis, 2D electrophoresis24, 27
Mycoplasma pneumoniaePrkCHMW1-3CytadherenceNAPro-Q Diamond staining of cell lysates155
MPN474Surface proteinNAPro-Q Diamond staining of cell lysates155
P1AdhesinNAPro-Q Diamond staining of cell lysates155
Myxococcus xanthusPkn2HUαHistone-like proteinT59Suppressor screen in E. coli, in vitro kinase assay, site-directed mutagenesis182
β-LactamaseAmpicillin resistanceThr(s)Phospho-amino acid analysis183
Pkn4PFKGlycolysisT226In vitro kinase assay, site-directed mutagenesis117
Pkn8Pkn14Soluble eSTKThr(s)In vitro kinase assay119
Pkn14MrpCTranscription factor; fruiting body developmentThr(s)In vitro kinase assay119
PFKGlycolysisT226In vitro kinase assay, site-directed mutagenesis, phospho-amino acid analysis117
Pseudomonas aeruginosaPpkAFha1Protein secretion, virulenceT362In vitro kinase assay, mass spectrometry, site-directed mutagenesis66, 113
Staphylococcus aureusPknBAFT-2Human transcription factorThr73Peptide arrays, in vitro kinase assay, mass spectrometry107
MgrAGlobal transcriptional regulatorNAIn vitro kinase assay181
PurAPurine biosynthesisNAIn vitro kinase assay39
SarATranscription regulator; virulenceThr(s)In vitro kinase assay, phospho-amino acid analysis38
SA0498Ribosomal protein L7/L12; central metabolismThr(s), Ser(s)In vitro kinase assay, phospho-amino acid analysis96
SA0545Phosphate acetyltransferase; central metabolismThr(s), Ser(s)In vitro kinase assay, phospho-amino acid analysis96
SA0729Triose isomerase; central metabolismThr(s)In vitro kinase assay, phospho-amino acid analysis96
SA0731Enolase; central metabolismThr(s), Ser(s)In vitro kinase assay, phospho-amino acid analysis96
SA0944Pyruvate dehydrogenase; central metabolismThr(s)In vitro kinase assay, phospho-amino acid analysis96
SA1359Elongation factor P; central metabolismThr(s), Ser(s)In vitro kinase assay, phospho-amino acid analysis96
SA1499Trigger factor; central metabolismThr(s), Ser(s)In vitro kinase assay, phospho-amino acid analysis96
SA2340Glyoxalase; central metabolismThr(s), Ser(s)In vitro kinase assay, phospho-amino acid analysis96
SA2399Fructose biphosphate aldolase; central metabolismThr(s)In vitro kinase assay, phospho-amino acid analysis96
Streptococcus agalactiaeStp1CovRResponse regulator; toxin expressionT65In vitro kinase assay93, 145
DivIACell divisionNAIn vivo phosphopeptide enrichment approach, in vitro kinase assay168
PpaCMn-dependent inorganic pyrophosphatase; virulenceSer(s)In vitro kinase assay, phospho-amino acid analysis144
PurAPurine biosynthesisNAIn vitro kinase assay146
Streptococcus pneumoniaeStkPDivIVACell divisionThr(s)In vivo 2D electrophoresis, mass spectrometry, in vitro kinase assay124
FtsZCell divisionThr(s)In vitro kinase assay55
GlmMCell wallNA2D electrophoresis, in vitro kinase assay125
PpaCMn-dependent inorganic pyrophosphatase; virulenceThr(s)In vivo 2D electrophoresis, mass spectrometry, in vitro kinase assay124
RitRTranscriptional regulator; iron transportNAIn vitro kinase assay184
Spr0334NAThr(s)In vivo 2D electrophoresis, mass spectrometry, in vitro kinase assay124
Streptococcus pyogenesSP-STKSP-HLPHistone-like proteinThr(s)In vitro kinase assay72
Streptomyces coelicolorAfsKAfsRTranscriptional factor; secondary metabolismSer(s), Thr(s)In vitro kinase assay102
Yersinia spp.YpkAEukaryotic actinCytoskeletonNAIn vitro kinase assay74
  • a NA, not available.