TABLE 3

Strain variability in studies in which collections of three or more isolates were analyzed in parallela

Authors, yr (reference)Mediumd (sugar)SubstrateNo. of strainsAssayMean ± SDSD as % of meanRangeFold difference of extremes
Hawser and Douglas, 1994 (168)YNB medium (glucose)Catheter13Dry wt (mg)0.82 ± 0.20240.52–1.102.1
[3H]leucine incorporation4,476 ± 1,058242,550–5,8862.3
MTT A5400.52 ± 0.11210.38–0.663.2
YNB medium (galactose)Catheter13Dry wt (mg)1.76 ± 0.32180.51–1.992.3
[3H]leucine incorporation8,503 ± 2,684322,672–10,2253.8
MTT A5400.98 ± 0.27280.49–1.243.2
Kuhn et al., 2002 (26)YNB mediumCatheter10Relative dry wt0.82 ± 0.23290.55–1.302.4
Relative XTT1.02 ± 0.30300.65–1.241.9
Li et al., 2003 (228)YNB mediumTCP16 (oral)XTT A4900.077 ± 0.052620.053–0.32962
CV A5700.209 ± 0.4241590.050–2.17943.4
31 (environmental)XTT A4900.079 ± 0.025320.55–0.1582.9
CV A5700.217 ± 0.2461130.087–1.03211.9
37 (vaginal)XTT A4900.063 ± 0.020320.049–0.1573.2
CV A5700.143 ± 0.081570.043–0.52612.2
Jain et al., 2007 (258)RPMI 1640eTCP63Relative XTT0.55 ± 0.059
UrineTCP63Relative XTT1.00 ± 0.2020
Villar-Vidal et al., 2011 (259)bRPMI 1640eCatheter16 (oral)XTT A4921.01 ± 0.40400.30–1.334.4
Urine12 (blood)XTT A4920.90 ± 0.37410.30–1.505.0
Pujol et al., 2015 (45)cRPMI 1640eCatheter3 (a/α)Thickness (mm)76 ± 2374–781.1
Catheter6 (a/a)Thickness (mm)56 ± 3551–581.2
Catheter6 (α/α)Thickness (mm)58 ± 2356–611.1
Catheter10 (a/α + α/α)Dry wt (mg)0.75 ± 0.13170.48–0.982.0
  • a TCP, tissue culture plastic; MTT A540, MTT reduction as measured by the absorbance at 540 nm; relative dry weight, dry weight relative to the wild type or the mean; relative XTT, XTT measure relative to the wild type or the mean; CV A570, crystal violet staining as measured by the absorbance at 570 nm; XTT A490 or A492, XTT reduction as measured at 490 nm or 492 nm; —, data not provided for calculation.

  • b The only study in which sampling occurred at 24 h, not 48 h.

  • c The only study performed at 28°C, not 37°C, because of white cell induction at temperatures of ≥35°C.

  • d Sugar contents for the different studies were as follows: for the study of Hawser and Douglas (168), 50 mM glucose or 500 mM galactose; for the study of Kuhn et al. (26), 50 mM glucose; for the study of Jain et al. (257), 11 mM glucose; for the study of Li et al. (227), 50 mM glucose; for the study of Villar-Vidal et al. (258), 11 mM glucose; and for the study of Pujol et al. (45), 11 mM glucose.

  • e Buffered with MOPS.